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Volume 147, Issue 8

A -like gene family in the pathogenic fungus

The EMBL accession numbers for the sequences reported in this paper are AJ302061 for , AJ302062 for and AJ302063 for

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Abstract

In fungi, the cell wall plays a major role in host–pathogen interactions. Despite this, little is known about the molecular basis of cell wall assembly in , which has emerged as the second most common cause of systemic candidosis. A gene family, , that shares significant homologies with both the gene of , which is necessary for cell wall assembly, and the pH-regulated genes and of , which are involved in cell wall assembly and required for virulence, has been cloned. Among the members of this family, display a unique expression pattern. Both and are constitutively expressed. In contrast, transcript was not detectable under any of the assayed conditions. The actin gene, , has also been cloned to be used as a meaningful loading control in Northern blots. and were deleted by two different methodological approaches. A rapid PCR-based strategy by which gene disruption was achieved with short regions of homology (50 bp) was applied successfully to . Δ or Δ cells demonstrated similar aberrant morphologies, displaying an altered bud morphology and forming floccose aggregates. These phenotypes suggest a role for and in cell wall biosynthesis. Further evidence for this hypothesis was obtained by successful functional complementation of a null mutation in with the or gene.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): actin , cell wall , CHEF, contour-clamped homogeneous electric field gel electrophoresis , GAS1 , GPI, glycosylphosphatidylinositol , PHR1 and PHR2
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2001-08-01
2024-04-20
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A GAS-like gene family in the pathogenic fungus Candida glabrataThe EMBL accession numbers for the sequences reported in this paper are AJ302061 for CgGAS1, AJ302062 for CgGAS2 and AJ302063 for CgGAS3.
Microbiology 147, 2007 (2001); https://doi.org/10.1099/00221287-147-8-2007
/content/journal/micro/10.1099/00221287-147-8-2007
/content/journal/micro/10.1099/00221287-147-8-2007
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