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Volume 147, Issue 8

Serine/threonine protein kinases PknF and PknG of : characterization and localization Free

Abstract

Pathogenesis of is closely connected to its survival and replication within the host. Some pathogenic bacteria employ protein kinases that interfere with the cellular signalling network of host cells and promote bacterial survival. In this study, the and genes, which encode two putative protein kinases of HRv, protein kinase F (PknF) and protein kinase G (PknG), respectively, were cloned and expressed in . Purified PknF phosphorylated the peptide substrate myelin basic protein (MBP) at serine and threonine residues, while purified PknG phosphorylated only at serine residues. The activity of the two kinases was abrogated by mutation of the codon for the predicted ATP-binding-site lysine residue. Southern blot analysis revealed that homologues of the genes encoding the two kinases are present in HRa and BCG, but not in . Immunoblot analysis of various cellular fractions of HRv revealed that PknF is a transmembrane protein and that PknG is predominantly a cytosolic enzyme. The present study should aid in elucidating the role of these protein kinases in the pathogenesis of mycobacteria.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): glutathione S-transferase , GST, glutathione S-transferase , MBP, myelin basic protein , mycobacteria , protein phosphorylation , transmembrane and tuberculosis
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2001-08-01
2024-04-25
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Serine/threonine protein kinases PknF and PknG of Mycobacterium tuberculosis: characterization and localization
Microbiology 147, 2307 (2001); https://doi.org/10.1099/00221287-147-8-2307
/content/journal/micro/10.1099/00221287-147-8-2307
/content/journal/micro/10.1099/00221287-147-8-2307
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