1887

Abstract

Genotypic characterization, based on the analysis of restriction fragment length polymorphism of the gene fragment PCR product ( PCR-RFLP), was performed on members of the former genus. PCR primers deduced from published gene sequences of allowed the amplification of an approximately 730 bp DNA fragment from each of the 19 species tested. Amplified fragments were compared using RFLP analysis with four endonucleases (I, fI, I and 1I), allowing the detection of characteristic patterns of RFLP products for most of the species. Between one and three specific RFLP groups were identified among most of the species tested (, , , , , , , , , , , , , , , , , subsp. , subsp. , subsp. and subsp. ). However, in two cases, and subsp. , 15 and 18 specific RFLP groups were detected, respectively. The variability of genetic patterns within these bacteria could be explained in terms of their geographic origin and/or wide host-range. The results indicated that PCR-RFLP analysis of the gene fragment is a useful tool for identification of species and subspecies belonging to the former genus, as well as for differentiation of strains within subsp. and

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2002-02-01
2024-03-28
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References

  1. Alcorn S. M., Orum T. V., Steigerwalt A. G., Foster J. L. M., Fogleman J. C., Brenner D. J. 1991; Taxonomy and pathogenicity of Erwinia cacticida sp. nov. Int J Syst Bacteriol 41:197–212 [CrossRef]
    [Google Scholar]
  2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A. Struhl K. editors 1992 Short Protocols in Molecular Biology , 2nd edn. New York: Greene Publishing Associates and Wiley;
    [Google Scholar]
  3. Beji A., Mergaert J., Gavini F., Izard D., Kersters K., Leclerc H., De Ley J. 1988; Subjective synonymy of Erwinia herbicola , Erwinia milletiae , and Enterobacter agglomerans and redefinition of the taxon by genotypic and phenotypic data. Int J Syst Bacteriol 38:77–88 [CrossRef]
    [Google Scholar]
  4. Bereswill S., Pahl A., Bellemann P., Zeller W., Geider K. 1992; Sensitive and species-specific detection of Erwinia amylovora by PCR-analysis. Appl Environ Microbiol 58:3522–3526
    [Google Scholar]
  5. Bereswill S., Bugert P., Geider K., Bruchmüller I. 1995; Identification of the fire blight pathogen, Erwinia amylovora , by PCR with chromosomal DNA. Appl Environ Microbiol 61:2636–2642
    [Google Scholar]
  6. Blackwood K. S., He C., Gunton J., Turenne C. Y., Wolfe J., Kabani A. M. 2000; Evaluation of recA sequences for identification of Mycobacterium species. J Clin Microbiol 38:2846–2852
    [Google Scholar]
  7. Brenner D. J., Fanning G. R., Steigerwalt A. G. 1974; Deoxyribonucleic acid relatedness among Erwiniae and other Enterobacteriaceae : the gall, wilt, and dry-necrosis organisms (Genus Erwinia Winslow et al. , sensu stricto. Int J Syst Bacteriol 24:197–204 [CrossRef]
    [Google Scholar]
  8. Brenner D. J., Fanning G. R., Leete Knutson J. K., Steigerwalt A. G., Krichevsky M. I. 1984; Attempts to classify herbicola group- Enterobacter agglomerans strains by deoxyribonucleic acid hybridization and phenotypic tests. Int J Syst Bacteriol 34:45–55 [CrossRef]
    [Google Scholar]
  9. Darrasse A., Priou S., Kotoujansky A., Bertheau Y. 1994; PCR and restriction fragment length polymorphism of a pel gene as a tool to identify Erwinia carotovora in relation to potato diseases. Appl Environ Microbiol 60:1437–1443
    [Google Scholar]
  10. De Boer S. H., McNaughton M. E. 1987; Monoclonal antibodies to the lipopolysaccharide of Erwinia carotovora subsp . atroseptica serogroup I. Phytopathology 77:828–832 [CrossRef]
    [Google Scholar]
  11. De Boer S. H., Ward L. J. 1995; PCR detection of Erwinia carotovora subsp. atroseptica associated with potato tissue. Phytopathology 85:854–858 [CrossRef]
    [Google Scholar]
  12. Dye D. W. 1968; A taxonomic study of the genus Erwinia . I. The ‘Amylovora’ group. N Z J Sci 11:590–607
    [Google Scholar]
  13. Dye D. W. 1969a; A taxonomic study of the genus Erwinia . II. The ‘Carotovora’ group. N Z J Sci 12:81–97
    [Google Scholar]
  14. Dye D. W. 1969b; A taxonomic study of the genus Erwinia . III. The ‘Herbicola’ group. N Z J Sci 12:223–236
    [Google Scholar]
  15. Dye D. W. 1969c; A taxonomic study of the genus Erwinia . IV. ‘Atypical’ erwinias. N Z J Sci 12:833–839
    [Google Scholar]
  16. Eisen J. A. 1995; The RecA protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of RecAs and 16S rRNAs from the same species. J Mol Evol 41:1105–1123
    [Google Scholar]
  17. Fréchon D., Exbrayat P., Helias V. 10 other authors 1998; Evaluation of a PCR kit for the detection of Erwinia carotovora subsp. atroseptica on potato tubers. Potato Res 41:163–173 [CrossRef]
    [Google Scholar]
  18. Gavini F., Mergaert J., Beji A., Mielcarek C., Izard D., Kersters K., De Ley J. 1989; Transfer of Enterobacter agglomerans (Beijerinck 1888) Ewing and Fife 1972 to Pantoea gen.nov. as Pantoea agglomerans comb. nov. and description of Pantoea dispersa sp. nov. Int J Syst Bacteriol 39:337–345 [CrossRef]
    [Google Scholar]
  19. Gorris M. T., Alarcon B., Lopez M. M., Cambra M. 1994; Characterization of monoclonal antibodies specific for Erwinia carotovora subsp. atroseptica and comparison of serological methods for its sensitive detection on potato tubers. Appl Environ Microbiol 60:2076–2085
    [Google Scholar]
  20. Guilford P. J., Taylor R. K., Clark R. G., Hale C. N., Forster R. L. 1996; PCR-based techniques for the detection of Erwinia amylovora . Acta Hortic 411:53–56
    [Google Scholar]
  21. Hao M. V., Brenner D. J., Steigerwalt A. G., Kosako Y., Komagata K. 1990; Erwinia persicinus , a new species isolated from plants. Int J Syst Bacteriol 40:379–383 [CrossRef]
    [Google Scholar]
  22. Hauben L., Moore E. R. B., Vauterin L., Steenackers M., Verdonck L., Swings J. 1998; Phylogenetic position of phytopathogens within the Enterobacteriacae . Syst Appl Microbiol 21:384–397 [CrossRef]
    [Google Scholar]
  23. Hyman L. J., Wallace A., Lopez M. M., Cambra M., Gorris M. T., Pérombelon, M. C. M. 1995; Characterization of monoclonal antibodies against Erwinia carotovora subsp. atroseptica serogroup I: specificity and epitope analysis. J Appl Bacteriol 78:437–444 [CrossRef]
    [Google Scholar]
  24. Karlin S., Weinstock G. M., Brendel V. 1995; Bacterial classification derived from RecA protein sequence comparisons. J Bacteriol 177:6881–6893
    [Google Scholar]
  25. Kim W. S., Gardan L., Rhim S. L., Geider K. 1999; Erwinia pyrifoliae sp. nov., a novel pathogen that affects Asian pear trees ( Pyrus pyrifolia Nakai. Int J Syst Bacteriol 49:899–906 [CrossRef]
    [Google Scholar]
  26. Kwon S. W., Go S. J., Kang H. W., Ryu J. C., Jo J. K. 1997; Phylogenetic analysis of Erwinia species based on 16S rRNA gene sequences. Int J Syst Bacteriol 47:1061–1067 [CrossRef]
    [Google Scholar]
  27. Lelliott R. A., Dickey R. S. 1984; Genus VII. Erwinia Winslow, Broadhurst, Buchanan, Krumwiede, Rogers and Smith 1920. In Bergey’s Manual of Systematic Bacteriology pp 469–476 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  28. Lind E., Ursing J. 1986; Clinical strains of Enterobacter agglomerans (synonyms: Erwinia herbicola , Erwinia milletiae ) identified by DNA–DNA hybridization. Acta Pathol Microbiol Immunol Scand Sect B 94:205–213
    [Google Scholar]
  29. Lloyd A. T., Sharp P. M. 1993; Evolution of the recA gene and molecular phylogeny of bacteria. J Mol Evol 37:399–407
    [Google Scholar]
  30. Ludwig W., Schleifer K. H. 1999; Phylogeny of bacteria beyond the 16 rRNA standard. ASM News 65:752–757
    [Google Scholar]
  31. McManus P. S., Jones A. L. 1995; Detection of Erwinia amylovora by nested PCR and PCR-dot-blot and reverse-blot hybridisations. Phytopathology 85:618–623 [CrossRef]
    [Google Scholar]
  32. Mahenthiralingam E., Bischof J., Byrne S. K., Radomski C., Davies J. E., Av-Gay Y., Vandamme P. 2000; DNA-based diagnostic approaches for identification of Bulkholderia cepacia complex, Bulkholderia vietnamiensis , Bulkholderia multivorans , Bulkholderia stabilis , and Bulkholderia cepacia genomovars I and III. J Clin Microbiol 38:3165–3173
    [Google Scholar]
  33. Mergaert J., Verdonck L., Kersters K., Swings J., Boeufgras J. M., De Ley J. 1984; Numerical taxonomy of Erwinia species using API systems. J Gen Microbiol 130:1893–1910
    [Google Scholar]
  34. Mergaert J., Verdonck L., Kersters K. 1993; Transfer of Erwinia ananas (synonym, Erwinia uredovora ) and Erwinia stewartii to the genus Pantoea emend. as Pantoea ananas (Serrano 1928) comb.nov. and Pantoea stewartii (Smith 1898) comb. nov., respectively, and description of Pantoeastewartii subsp. indologenes subsp. nov. . Int J Syst Bacteriol 43:162–173 [CrossRef]
    [Google Scholar]
  35. Mergaert J., Hauben L., Cnockaert M. C., Swings J. 1999; Reclassification of non-pigmented Erwinia herbicola strains from trees as Erwinia billingiae sp. nov. Int J Syst Bacteriol 49:377–383 [CrossRef]
    [Google Scholar]
  36. Nassar A., Darrasse A., Lemattre M., Kotoujansky A., Dervin C., Vedel R., Bertheau Y. 1996; Characterization of Erwinia chrysanthemi by pectinolytic isozyme polymorphism and restriction fragment length polymorphism analysis of PCR-amplified fragments of pel genes. Appl Environ Microbiol 62:2228–2235
    [Google Scholar]
  37. Nowak A., Kur J. 1995; Genomic species typing of acinetobacters by polymerase chain reaction amplification of the recA gene. FEMS Microbiol Lett 130:327–332 [CrossRef]
    [Google Scholar]
  38. Perombelon M. C. M., Kelman A. 1980; Ecology of the soft rot erwinias. Annu Rev Phytopathol 18:361–387 [CrossRef]
    [Google Scholar]
  39. Salaman R. 1949 The History and Social Influence of the Potato Cambridge: Cambridge University Press;
    [Google Scholar]
  40. Samson R., Ngwira N., Rivera N. 1989; Biochemical and serological diversity of Erwinia chrysanthemi . In Proceedings of the 7th International Conference on Plant Pathogenic Bacteria pp 895–901 Edited by Klement Z. Budapest: Akademidi Kiadó és Nyomoda Vállalat;
    [Google Scholar]
  41. Schroth M., Thomson S. V., Hildebrand D. C. 1974; Epidemiology and control of fireblight. Annu Rev Phytopathol 12:389–412 [CrossRef]
    [Google Scholar]
  42. Smid E. J., Jansen A. H. J., Gorris L. G. M. 1995; Detection of Erwinia carotovora subsp. atroseptica and Erwinia chrysanthemi in potato tubers using polymerase chain reaction. Plant Pathol 44:1058–1069 [CrossRef]
    [Google Scholar]
  43. Śledź W., Jafra S., Waleron M., Lojkowska E. 2000; Genetic diversity of Erwinia carotovora strains isolated from infected plants grown in Poland. EPPO Bull 30:403–407 [CrossRef]
    [Google Scholar]
  44. Toth I. K., Hyman L. J., Wood J. R. 1999a; A one step PCR-based method for the detection of economically important soft rot Erwinia species on micropropagated potato plants. J Appl Microbiol 87:158–166 [CrossRef]
    [Google Scholar]
  45. Toth I. K., Bertheau Y., Hyman L. J. 9 other authors 1999b; Evaluation of phenotypic and molecular typing techniques for determining diversity in Erwinia carotovora subsp. atroseptica . J Appl Microbiol 87:770–781 [CrossRef]
    [Google Scholar]
  46. Vantomme R., Swings J., Goor M., Kersters K., De Ley J. 1982; Phytopathological, serological, biochemical and protein electrophoretic characterization of Erwinia amylovora strains isolated in Belgium. Phytopathol Z 103:349–360 [CrossRef]
    [Google Scholar]
  47. Verdonck L., Mergaert J., Rijckaert C., Swings J., Kersters K., De Ley J. 1987; The genus Erwinia : a numerical analysis of phenotypic features. Int J Syst Bacteriol 37:4–18 [CrossRef]
    [Google Scholar]
  48. Waleron M., Waleron K., Łojkowska E. 2001; Application of recA PCR-RFLP for differentiation of Erwinia carotovora associated with potato plants in tuber. In Proceedings of the 10th International Conference on Plant Pathogenic Bacteria pp 385–388 Charlottetown, Canada. Dordrecht: Kluwer;
    [Google Scholar]
  49. Watanabe K., Sato M. 1998; Detection of variation of the R-domain structure of ice nucleation genes in Erwinia herbicola -group bacteria by PCR-RFLP analysis. Curr Microbiol 37:201–209 [CrossRef]
    [Google Scholar]
  50. Willson W. J., Wiedmann M., Dillard H. R., Batt C. A. 1994; Identification of Erwinia stewartii by a ligase chain reaction assay. Appl Environ Microbiol 60:278–284
    [Google Scholar]
  51. Zhang Y., Geider K. 1997; Differentiation of Erwinia amylovora strains by pulsed-field gel electrophoresis. Appl Environ Microbiol 63:4421–4426
    [Google Scholar]
  52. Zhao X., McEntee K. 1990; DNA sequence analysis of the recA genes from Proteus vulgaris , Erwinia carotovora , Shigella flexneri and Escherichia coli B/r. Mol Gen Genet 222:369–376 [CrossRef]
    [Google Scholar]
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