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Volume 148, Issue 3

The positive activator of cephamycin C and clavulanic acid production in is mistranslated in a mutant

The GenBank accession number for the sequence reported in this paper is AF436078.

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Abstract

In encodes the principal leucyl tRNA for translation of UUA codons and controls pigmented antibiotic production by the presence of TTA codons in the genes encoding the pathway-specific activators of actinorhodin and undecylprodigiosin biosynthesis. In the gene encoding the pathway-specific activator of both cephamycin C and clavulanic acid production, , also contains a TTA codon and was expected to exhibit -dependence. A cloned DNA fragment containing a sequence showing 91% identity to the -encoded tRNA was able to restore antibiotic production and sporulation to mutants of and the closely related . A null mutation of the gene in resulted in the expected sporulation defective phenotype, but unexpectedly had no effect on antibiotic production. Transcript analysis showed no difference in the levels of transcripts in the wild-type and mutant strains, ruling out any effect of elevated levels of the mRNA. Furthermore, when compared to the wild-type strain, the mutant showed no differences in the levels of CcaR, suggesting that the single TTA codon in is mistranslated efficiently. The role of codon context in dependence is discussed.

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  • Accepted:
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  • Published Online:
Keyword(s): antibiotic production , differentiation , GFP, green fluorescent protein , mistranslation , Streptomyces and tRNA
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2002-03-01
2024-04-24
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The positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus is mistranslated in a bldA mutantThe GenBank accession number for the sequence reported in this paper is AF436078.
Microbiology 148, 643 (2002); https://doi.org/10.1099/00221287-148-3-643
/content/journal/micro/10.1099/00221287-148-3-643
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