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Volume 148, Issue 6

A 5′ stem–loop and ribosome binding but not translation are important for the stability of leader mRNA Free

Abstract

The leader is a determinant of extreme mRNA stability. The authors examined what properties of the leader confer stability on an mRNA. The secondary structure of the leader mRNA was analysed and , and mutations were introduced into different domains of an leader– fusion. The half-lives of the corresponding transcripts were determined and β-galactosidase activities were measured. Removal of a stem–loop structure at the 5′ end or diminishing the strength of the RBS reduced the half-lives from more than 25 min to about 5 min. Interfering with translation by abolishing the start codon or creating an early stop codon had no or little effect on mRNA stability. The authors conclude that a 5′ stem–loop and binding of ribosomes are necessary for the stability of leader mRNA. The present results, together with a number of other data, suggest that translation of a . mRNA is generally not important for its stability; the situation seems different in . It is further concluded that the calculated strength of a . RBS cannot be used to predict the stability of the corresponding transcript.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): DMS, dimethyl sulphate , mRNA degradation and stability determinants
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2002-06-01
2024-04-18
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A 5′ stem–loop and ribosome binding but not translation are important for the stability of Bacillus subtilis aprE leader mRNA
Microbiology 148, 1795 (2002); https://doi.org/10.1099/00221287-148-6-1795
/content/journal/micro/10.1099/00221287-148-6-1795
/content/journal/micro/10.1099/00221287-148-6-1795
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