1887

Abstract

Kid is a small protein that is encoded by plasmid R1. It is a toxin that belongs to a killer system that ensures the stability of the plasmid in host cells. The results of previous studies have suggested that Kid is an inhibitor of DNA replication, possibly acting at the onset of initiation. Here, the authors tested the effects of Kid on λ-intitiated and -initiated replication, which may be driven by both the newly assembled replication complex and the heritable complex. It was found that Kid inhibits only replication that is driven by the newly assembled replication complex. The authors also report that Kid inhibits ColE1-like plasmid replication , in agreement with the previously reported inhibition of ColE1 during replication. It is proposed that the Kid toxin acts at the level of replication either by preventing assembly of the replication complex or by impairing the functional interactions of the replication complex at the initiation stage.

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2002-08-01
2024-04-20
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References

  1. Allen G. C. Jr, Kornberg A. 1991; Fine balance in the regulation of DnaB helicase by DnaC protein in replication in Escherichia coli . J Biol Chem 266:22096–22101
    [Google Scholar]
  2. Barańska S., Konopa G., Wȩgrzyn G. 2002; Directionality of λ plasmid DNA replication carried out by the heritable replication complex. Nucleic Acids Res 30:1176–1181 [CrossRef]
    [Google Scholar]
  3. Bolivar F., Backman K. 1979; Plasmids of Escherichia coli as cloning vectors. Methods Enzymol 68:245–267
    [Google Scholar]
  4. Boyd A. C., Sherratt D. J. 1995; The pCLIP plasmids: versatile cloning vectors based on the bacteriophage lambda origin of replication. Gene 153:57–62 [CrossRef]
    [Google Scholar]
  5. Bravo A., de Torrontegui G., Diaz R. 1987; Identification of components of a new stability system of plasmid R1, parD , that is close to the origin of replication of this plasmid. Mol Gen Genet 210:101–110 [CrossRef]
    [Google Scholar]
  6. Bravo A., Ortega S., de Torrontegui G., Diaz R. 1988; Killing of Escherichia coli cells modulated by components of the stability system parD of plasmid R1. Mol Gen Genet 215:146–151 [CrossRef]
    [Google Scholar]
  7. Cashel M., Gentry D. R., Hernandez V. J., Vinella D. others 1996; The stringent response. In Escherichia coli and Salmonella: Cellular and Molecular Biology, 2nd edn. pp 1458–1496 Edited by Neidhardt F. C. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Diaz R., Pritchard R. H. 1978; Cloning of replication origins from E. coli K12 chromosome. Nature 275:561–564 [CrossRef]
    [Google Scholar]
  9. Gotfredsen M., Gerdes K. 1998; The Escherichia coli relBE genes belong to a new toxin–antitoxin gene family. Mol Microbiol 29:1065–1076 [CrossRef]
    [Google Scholar]
  10. Herman A., Wȩgrzyn A., Wȩgrzyn G. 1994a; Differential replication of plasmids during stringent and relaxed response of Escherichia coli . Plasmid 32:89–94 [CrossRef]
    [Google Scholar]
  11. Herman A., Wȩgrzyn A., Wȩgrzyn G. 1994b; Regulation of replication of plasmid pBR322 in amino acid-starved Escherichia coli strains. Mol Gen Genet 243:374–378
    [Google Scholar]
  12. Jensen K. F. 1993; The Escherichia coli K-12 ‘wild types’ W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J Bacteriol 175:3401–3407
    [Google Scholar]
  13. Lanzer M., Bujard H. 1988; Promoters largely determine the efficiency of repressor action. Proc Natl Acad Sci USA 85:8973–8977 [CrossRef]
    [Google Scholar]
  14. Mechold U., Cashel M., Steiner K., Gentry D., Malke H. 1996; Functional analysis of a relA / spoT gene homolog from Streptococcus equisimilis . J Bacteriol 178:1401–1411
    [Google Scholar]
  15. Potrykus K., Wróbel B., Wȩgrzyn A., Wȩgrzyn G. 2000; Replication of oriJ -based plasmid DNA during the stringent and relaxed responses of Escherichia coli . Plasmid 44:111–126 [CrossRef]
    [Google Scholar]
  16. Potrykus K., Barańska S., Wȩgrzyn A., Wȩgrzyn G. 2002; Composition of the λ plasmid heritable replication complex. Biochem J 364:857–862 [CrossRef]
    [Google Scholar]
  17. Ruiz-Echevarria M. J., Gimenez-Gallego G., Sabariegos-Jareno R., Diaz-Orejas R. 1995; Kid, a small protein of the parD stability system of plasmid R1, is an inhibitor of DNA replication acting at the initiation of DNA synthesis. J Mol Biol 247:568–577
    [Google Scholar]
  18. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Szalewska-Pałasz A., Wȩgrzyn A., Herman A., Wȩgrzyn G. 1994; The mechanism of the stringent control of λ plasmid DNA replication. EMBO J 13:5779–5785
    [Google Scholar]
  20. Wȩgrzyn G., Taylor K. 1992; Inheritance of the replication complex by one of two daughter copies during λ plasmid replication in Escherichia coli . J Mol Biol 226:681–688 [CrossRef]
    [Google Scholar]
  21. Wȩgrzyn A., Wȩgrzyn G. 1995; Transcriptional activation of ori λ regulates λ plasmid replication in amino acid-starved Escherichia coli cells. Biochem Biophys Res Commun 214:978–984 [CrossRef]
    [Google Scholar]
  22. Wȩgrzyn A., Wȩgrzyn G. 2001; Inheritance of the replication complex: a unique or common phenomenon in the control of DNA replication?. Arch Microbiol 175:86–93 [CrossRef]
    [Google Scholar]
  23. Wȩgrzyn G., Neubauer P., Krueger S., Hecker M., Taylor K. 1991; Stringent control of replication of plasmids derived from coliphage λ. Mol Gen Genet 225:94–98
    [Google Scholar]
  24. Wȩgrzyn G., Pawłowicz A., Taylor K. 1992; Stability of coliphage λ DNA replication initiator, the λO protein. J Mol Biol 226:675–680 [CrossRef]
    [Google Scholar]
  25. Wȩgrzyn A., Wȩgrzyn G., Taylor K. 1995a; Protection of coliphage λO initiator protein from proteolysis in the assembly of the replication complex in vivo . Virology 207:179–184 [CrossRef]
    [Google Scholar]
  26. Wȩgrzyn A., Wȩgrzyn G., Taylor K. 1995b; Plasmid and host functions required for λ plasmid replication carried out by the inherited replication complex. Mol Gen Genet 247:501–508 [CrossRef]
    [Google Scholar]
  27. Wȩgrzyn A., Wȩgrzyn G., Herman A., Taylor K. 1996; Protein inheritance: λ plasmid replication perpetuated by the heritable replication complex. Genes Cells 1:953–963 [CrossRef]
    [Google Scholar]
  28. Wróbel B., Wȩgrzyn G. 1997; Differential amplification efficiency of pMB1 and p15A (ColE1-type) replicons in Escherichia coli stringent and relaxed strains starved for particular amino acids. Microbiol Res 152:251–255 [CrossRef]
    [Google Scholar]
  29. Wróbel B., Wȩgrzyn G. 1998; Replication regulation of ColE1-like plasmids in amino acid-starved Escherichia coli . Plasmid 39:48–62 [CrossRef]
    [Google Scholar]
  30. Wróbel B., Murphy H., Cashel M., Wȩgrzyn G. 1998; Guanosine tetraphosphate (ppGpp)-mediated inhibition of the activity of the bacteriophage λ p R promoter in Escherichia coli . Mol Gen Genet 257:490–495 [CrossRef]
    [Google Scholar]
  31. Żylicz M., Liberek K., Wawrzynów A., Georgopoulos C. 1998; Formation of the preprimosome protects λO from RNA transcription-dependent proteolysis by ClpP/ClpX. Proc Natl Acad Sci USA 95:15259–15263 [CrossRef]
    [Google Scholar]
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