Priming reverse transcription with oligo(dT) does not yield representative samples of Mycobacterium tuberculosis cDNA

References

1. Adilakshmi T., Ayling P. D., Ratledge C. 2000; Polyadenylation in mycobacteria: evidence for oligo(dT)-primed cDNA synthesis. Microbiology 146:633–638
   [Google Scholar]
2. Alland D., Kramnik I., Weisbrod T. R., Otsubo L., Cerny R., Miller L. P., Jacobs W. R. Jr, Bloom B. R. 1998; Identification of differentially expressed mRNA in prokaryotic organisms by customized amplified libraries (DECAL): the effect of isoniazid on gene expression in Mycobacterium tuberculosis . Proc Natl Acad Sci USA 95:13227–13232 [CrossRef]
   [Google Scholar]
3. Call D. R., Chandler D. P., Brockman F. 2001; Fabrication of DNA microarrays using unmodified oligonucleotide probes. Biotechniques 30:368–372
   [Google Scholar]
4. Cole S. T., Brosch R., Parkhill J. 39 other authors 1998; Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537–544 [CrossRef]
   [Google Scholar]
5. Dye C., Scheele S., Dolin P., Pathania V., Raviglione M. D. 1999; Global burden of tuberculosis – estimated incidence, prevalence, and mortality by country. JAMA (J Am Med Assoc) 282:677–686 [CrossRef]
   [Google Scholar]
6. Graham J. E., Clark-Curtiss J. E. 1999; Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS. Proc Natl Acad Sci USA 96:11554–11559 [CrossRef]
   [Google Scholar]
7. Kane M. D., Jatkoe T. A., Stumpt C. R., Lu J., Thomas J. D., Madore S. J. 2000; Assessment of the sensitivity and specificity of oligonucleotide (50mer. Nucleic Acids Res 28:4552–4557 [CrossRef]
   [Google Scholar]
8. Katoch V. M., Cox R. A. 1986; Step-wise isolation of RNA and DNA from mycobacteria. Int J Lepr 54:409–415
   [Google Scholar]
9. Mohanty B. K., Kushner S. R. 2000; Polynucleotide phosphorylase, Rnase II and Rnase E play different roles in the in vivo modulation of polyadenylation in Escherichia coli . Mol Microbiol 36:982–994 [CrossRef]
   [Google Scholar]
10. Rindi L., Lari N., Gil M. G., Garzelli C. 1998; Oligo(dT)-primed synthesis of cDNA by reverse transcriptase in mycobacteria. Biochem Biophys Res Commun 248:216–218 [CrossRef]
    [Google Scholar]
11. Rindi L., Lari N., Garzelli C. 1999; Search for genes potentially involved in Mycobacterium tuberculosis virulence by mRNA differential display. Biochem Biophys Res Commun 258:94–101 [CrossRef]
    [Google Scholar]
12. Sarkar N. 1996; Polyadenylation of mRNA in bacteria. Microbiology 142:3125–3133 [CrossRef]
    [Google Scholar]
13. Sarkar N. 1997; Polyadenylation of mRNA in prokaryotes. Annu Rev Biochem 66:173–197 [CrossRef]
    [Google Scholar]
14. Talaat A. M., Hunter P., Johnston S. A. 2000; Genome-directed primers for selective labeling of bacterial transcripts for DNA microarray analysis. Nat Biotechnol 66:679–682
    [Google Scholar]