1887

Abstract

Munumbicins A, B, C and D are newly described antibiotics with a wide spectrum of activity against many human as well as plant pathogenic fungi and bacteria, and a sp. These compounds were obtained from NRRL 3052, which is endophytic in the medicinal plant snakevine (), native to the Northern Territory of Australia. This endophyte was cultured, the broth was extracted with an organic solvent and the contents of the residue were purified by bioassay-guided HPLC. The major components were four functionalized peptides with masses of 12696, 12985, 13125 and 13265 Da. Numerous other related compounds possessing bioactivity, with differing masses, were also present in the culture broth extract in lower quantities. With few exceptions, the peptide portion of each component contained only the common amino acids threonine, aspartic acid (or asparagine), glutamic acid (or glutamine), valine and proline, in varying ratios. The munumbicins possessed widely differing biological activities depending upon the target organism. For instance, munumbicin B had an MIC of 25 μg ml against a methicillin-resistant strain of , whereas munumbicin A was not active against this organism. In general, the munumbicins demonstrated activity against Gram-positive bacteria such as and multidrug-resistant . However, the most impressive biological activity of any of the munumbicins was that of munumbicin D against the malarial parasite , having an IC of 45±007 ng ml. This report also describes the potential of the munumbicins in medicine and agriculture.

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2002-09-01
2024-04-19
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References

  1. Arai T. 1976 Actinomycetes: the Boundary Microorganisms Singapore: Toppan;
    [Google Scholar]
  2. Bacon C. W., White J. F. 2000 Microbial Endophytes New York: Marcel Dekker;
    [Google Scholar]
  3. Baldacci E., Farina G., Piacenza E., Fabbri G. 1968; Description of Streptomyces padanus sp. nov. and emendation of Streptomyces xantophaeus . Giorn Microbiol 16:9–16
    [Google Scholar]
  4. Ballio A., Bossa F., DiGiogio P., Ferranti P., Paci M., Pucci P., Scaloni A., Segre A., Strobel G. A. 1994; Structure of the pseudomycins, new lipodepsipeptides produced by Pseudomonas syringae MSU 16H. FEBS Lett 355:96–100 [CrossRef]
    [Google Scholar]
  5. Gurney K. A., Mantle P. G. 1993; Biosynthesis of 1- N -methylalbonoursin by an endophytic Streptomyces sp. J Nat Prod 56:1194–1198 [CrossRef]
    [Google Scholar]
  6. Isenberg H. D. editor 1992 Clinical Microbiology Procedures Handbook vol. 1 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  7. Li J. Y., Harper J. K., Grant D. M., Tombe B., Bashyal B., Hess W. M., Strobel G. A. 2001; Ambuic acid, a highly functionalized cyclohexenone with bioactivity from Pestalotiopsis spp. and Monochaetia sp. Phytochemistry 56:464–468
    [Google Scholar]
  8. Miller C. M., Miller R. V., Garton-Kinney D., Redgrave B., Sears J., Condron M., Teplow D., Strobel G. A. 1998; Ecomycins, unique antimycotics from Pseudomonas viridiflava . J Appl Microbiol 4:937–944
    [Google Scholar]
  9. National Institutes of Health 2001 NIAID Global Health Research Plan for HIV/AIDS, Malaria and Tuberculosis Bethesda, MD: US Department of Health and Human Services;
    [Google Scholar]
  10. Overton J. 1996 Ecologically Based Pest Mangement – New Solutions for a New Century Washington, DC: National Academy of Sciences Press;
    [Google Scholar]
  11. Pablosmendez A., Raviglione M. C., Laszlo A. 8 other authors 1997; Global surveillance for antituberculosis-drug resistance. N Engl J Med 338:1641–1649
    [Google Scholar]
  12. Raviglione M. C., Snider D. E., Kochi A. 1995; Global epidemiology of tuberculosis: morbidity and mortality of a worldwide epidemic. J Am Med Assoc 273:220–226 [CrossRef]
    [Google Scholar]
  13. Silverstein R. M., Bassler G. C., Morrill T. C. 1991 Spectrometric Identification of Organic Compounds New York: Wiley;
    [Google Scholar]
  14. Strobel G. A., Torczynski R., Bollon A. 1997; Acremonium sp. – a leucinostatin A producing endophyte of European yew ( Taxus baccata . Plant Sci 128:97–108 [CrossRef]
    [Google Scholar]
  15. Strobel G. A., Miller R. V., Miller C., Condron M., Teplow D. B., Hess W. M. 1999; Cryptocandin, a potent antimycotic from the endophytic fungus Cryptosporiopsis cf. quercina . Microbiology 145:1919–1926 [CrossRef]
    [Google Scholar]
  16. Tiphine M., Letscher V., Herbrecht R. 1999; Amphotericin B and its new formulations: pharmacologic characteristics, clinical efficacy, and tolerability. Transplant Infect Dis 1:273–283 [CrossRef]
    [Google Scholar]
  17. Trager W., Jensen J. B. 1976; Human malaria parasites in continuous culture. Science 193:673–675 [CrossRef]
    [Google Scholar]
  18. Trager W., Jensen J. B. 1978; Cultivation of malarial parasites. Nature 273:621–622 [CrossRef]
    [Google Scholar]
  19. Waksman S. A. 1967 The Actinomycetes New York: Ronald Press;
    [Google Scholar]
  20. Walsh T. A. 1992; Inhibitors of β-glucan synthesis. In Emerging Targets in Antibacterial and Antifungal Chemotherapy pp 349–373 Edited by Sutcliffe J. A., Georgopapadakou N. H. London: Chapman & Hall;
    [Google Scholar]
  21. Walsh T. A., Finberg R. W. 1999; Liposomal amphotericin B for therapy in patients with persistent fever and neutropenia. N Engl J Med 340:764–771 [CrossRef]
    [Google Scholar]
  22. Worapong J., Strobel G. A., Ford E. J., Li J. Y., Baird G., Hess W. M. 2001; Muscodor albus albus gen. et sp. nov., an endophyte from Cinnamomum zeylanicum . Mycotaxon 79:67–79
    [Google Scholar]
  23. Yang X., Strobel G., Stierle A., Hess W. M., Lee J., Clardy J. 1994; A fungal endophyte–tree relationship; Phoma sp. in Taxus wallichana . Plant Sci 102:1–9 [CrossRef]
    [Google Scholar]
  24. Young D. H., Michelotti E. J., Sivendell C. S., Krauss N. E. 1992; Antifungal properties of taxol and various analogues. Experientia 48:882–885 [CrossRef]
    [Google Scholar]
  25. Zhang Y. Z., Sun X., Zeckner D., Sachs B., Current W., Chen S. H. 2001a; 8-Amido-bearing pseudomycin B (PSB) analogue: novel antifungal agents. Bioorg Med Chem Lett 11:123–126 [CrossRef]
    [Google Scholar]
  26. Zhang Y. Z., Sun X., Zeckner D., Sachs B., Current W., Gidda J., Rodriguez M., Chen S. H. 2001b; Synthesis and antifungal activities of novel 3-amido bearing pseudomycin analogs. Bioorg Med Chem Lett 11:903–907 [CrossRef]
    [Google Scholar]
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