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Volume 141, Issue 7

A linear B-cell epitope on the class 3 outer-membrane protein of recognized after vaccination with the Norwegian group B outer-membrane vesicle vaccine Free

Abstract

Summary: The class 3 outer-membrane protein (OMP) of is a potential target for bactericidal and opsonic antibodies in humans. Synthetic peptides spanning the class 3 OMP from the vaccine strain 44/76 (B: 15:P1.7,16:L3,7) were synthesized on pins and screened with serum obtained from Norwegian adolescents immunized with a meningococcal serogroup B outer-membrane vesicle (OMV) vaccine. A strong IgG response to a single peptide (FHQNGQVTEVTT) located within loop 1 (VR1) was stimulated after three doses of OMV vaccine in three vaccinees selected on the basis of their antibody response to class 3 OMP. No clear linear B-cell epitopes were recognized by four different murine serotype 15-specific mAbs. A 23mer peptide (D63b2) containing loop 1 of the class 3 OMP was synthesized, and the IgG responses were measured in pre- and post-vaccination serum from 27 vaccinees. Specific IgG rose significantly in 37% of vaccinees 6 weeks after the second dose and in 74% of the vaccinees 6 weeks after the third dose of the OMV vaccine. Most immune sera reacted distinctly on immunoblots with denatured class 3 OMP, and the immunoblotting reactivity correlated strongly with concentration of the IgG antibodies specific for peptide D63b2. When added to a post-vaccination serum from one vaccinee, peptide D63b2 competed efficiently with the class 3 OMP for specific antibody binding on immunoblots and in pin ELISA. The results show that the significant part of the humoral response to the meningococcal class 3 OMP elicited by vaccination with the Norwegian OMV vaccine was directed against a single continuous epitope.

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Keyword(s): B-cell epitope , murine mAbs , Neisseria meningitidis PorB protein and synthetic peptides
© Society for General Microbiology 1995
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1995-07-01
2024-04-16
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A linear B-cell epitope on the class 3 outer-membrane protein of Neisseria meningitidis recognized after vaccination with the Norwegian group B outer-membrane vesicle vaccine
Microbiology 141, 1593 (1995); https://doi.org/10.1099/13500872-141-7-1593
/content/journal/micro/10.1099/13500872-141-7-1593
/content/journal/micro/10.1099/13500872-141-7-1593
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