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Volume 155, Issue 5

Characterization of the pathogenicity island protein PdpA and its role in the virulence of Free

Abstract

is a highly virulent, intracellular pathogen that causes the disease tularaemia. A research surrogate for is , which causes a tularaemia-like disease in mice, grows similarly in macrophages, and yet is unable to cause disease in humans. Both species contain a cluster of genes referred to as the pathogenicity island (FPI). Pathogenicity determinant protein A (PdpA), encoded by the gene, is located within the FPI and has been associated with the virulence of species. In this work we examined the properties of PdpA protein expression and localization as well as the phenotype of a deletion mutant. Monoclonal antibody detection of PdpA showed that it is a soluble protein that is upregulated in iron-limiting conditions and undetectable in an or mutant background. Deletion of resulted in a strain that was highly attenuated for virulence in chicken embryos and mice.

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Keyword(s): FPI, Francisella pathogenicity island and T6SS, type VI secretion system
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2009-05-01
2024-04-16
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Characterization of the pathogenicity island protein PdpA and its role in the virulence of Francisella novicida
Microbiology 155, 1489 (2009); https://doi.org/10.1099/mic.0.025379-0
/content/journal/micro/10.1099/mic.0.025379-0
/content/journal/micro/10.1099/mic.0.025379-0
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