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Volume 156, Issue 4

Cloning and expression analysis of the duplicated genes for carbon monoxide dehydrogenase of sp. strain JC1 DSM 3803 Free

Abstract

Carbon monoxide dehydrogenase (CO-DH) is an enzyme catalysing the oxidation of CO to carbon dioxide in sp. strain JC1 DSM 3803. Cloning of the genes encoding CO-DH from the bacterium and sequencing of overlapping clones revealed the presence of duplicated sets of genes for three subunits of the enzyme, and , in operons, and a cluster of genes encoding proteins that may be involved in CO metabolism, including a possible transcriptional regulator. Phylogenetic analysis based on the amino acid sequences of large subunits of CO-DH suggested that the CO-DHs of sp. JC1 and other mycobacteria are distinct from those of other types of bacteria. The growth phenotype of mutant strains lacking genes and of a corresponding complemented strain showed that both of the duplicated sets of CO-DH genes were functional in this bacterium. Transcriptional fusions of the genes with revealed that the operons were expressed regardless of the presence of CO and were further inducible by CO. Primer extension analysis indicated two promoters, one expressed in the absence of CO and the other induced in the presence of CO. This is believed to be the first report to show the presence of multiple copies of CO-DH genes with identical sequences and in close proximity in carboxydobacteria, and to present the genetic evidence for the function of the genes in mycobacteria.

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Keyword(s): CO-DH, carbon monoxide dehydrogenase , INT, 2-(4-indophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride and NO-DH, nitric oxide dehydrogenase
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2010-04-01
2024-04-20
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Cloning and expression analysis of the duplicated genes for carbon monoxide dehydrogenase of Mycobacterium sp. strain JC1 DSM 3803
Microbiology 156, 999 (2010); https://doi.org/10.1099/mic.0.034769-0
/content/journal/micro/10.1099/mic.0.034769-0
/content/journal/micro/10.1099/mic.0.034769-0
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