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Volume 158, Issue 9

Molecular characterization of a novel mosaic (S/M) gene encoding tetracycline resistance in foodborne strains of Free

Abstract

The presence of antibiotic-resistance (AR) genes in foodborne bacteria of enteric origin represents a relevant threat to human health in the case of opportunistic pathogens, which can reach the human gut through the food chain. is a human opportunistic pathogen often associated with infections in immune-compromised or cancer patients, and it can also be detected in the environment, including fermented foods. We have focused on the molecular characterization of a tetracycline (Tet)-resistance gene present in 39 foodborne isolates of phenotypically resistant to this drug. The gene was identified as a novel (S/M) fusion, encoding a mosaic protein composed of the N-terminal 33 amino acids of Tet(S), in-frame with the Tet(M) coding sequence. Heterologous expression of the mosaic gene was found to confer Tet resistance upon recipients. Moreover, the (S/M) gene was found to be transcriptionally inducible by Tet under the endogenous (S) promoter in both and Nucleotide sequencing of the surrounding genomic region of 16.2 kb revealed large blocks of homology with the genomes of and A subregion of about 4 kb containing mosaic (S/M) was flanked by two copies of the IS mobile element. PCR amplification with primers directed outwards from the (S/M) gene identified the presence of a 4.3 kb circular form corresponding to the intervening chromosomal region between the two IS elements, but lacking a replication origin. The circular element shared extensive overall homology with a region of the multidrug-resistance plasmid pK214 from , containing (S), as well as the IS transposase-containing element and intervening non-coding sequences. Linear reconstruction of the insertion events likely to have occurred within this genomic region, inferred from sequence homology, provides further evidence of the chromosomal rearrangements that drive genomic evolution in complex bacterial communities such as the gut and food microbiota.

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2012-09-01
2024-04-24
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Molecular characterization of a novel mosaic tet(S/M) gene encoding tetracycline resistance in foodborne strains of Streptococcus bovis
Microbiology 158, 2353 (2012); https://doi.org/10.1099/mic.0.058206-0
/content/journal/micro/10.1099/mic.0.058206-0
/content/journal/micro/10.1099/mic.0.058206-0
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