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Volume 160, Issue 4

Sulfate-dependent acetate oxidation under extremely natron-alkaline conditions by syntrophic associations from hypersaline soda lakes Free

Abstract

So far, anaerobic sulfate-dependent acetate oxidation at high pH has only been demonstrated for a low-salt-tolerant syntrophic association of a clostridium ‘ Contubernalis alkalaceticum’ and its hydrogenotrophic sulfate-reducing partner . Anaerobic enrichments at pH 10 inoculated with sediments from hypersaline soda lakes of the Kulunda Steppe (Altai, Russia) demonstrated the possibility of sulfate-dependent acetate oxidation at much higher salt concentrations (up to 3.5 M total Na). The most salt-tolerant purified cultures contained two major components apparently working in syntrophy. The primary acetate-fermenting component was identified as a member of the order forming, together with ‘ Contubernalis alkalaceticum’, an independent branch within the family . A provisional name, ‘ Syntrophonatronum acetioxidans’, is suggested for the novel haloalkaliphilic clostridium. Two phylotypes of extremely haloalkaliphilic sulfate-reducing bacteria of the genus were identified as sulfate-reducing partners in the acetate-oxidizing cultures under extreme salinity. The dominant phylotype differed from the two species of described so far, whilst a minor component belonged to . The results proved that, contrary to previous beliefs, sulfate-dependent acetate oxidation is possible, albeit very slowly, in nearly saturated soda brines.

  • Received:
  • Accepted:
  • Published Online:
Funding
This study was supported by the:
  • Russian Foundation for Basic Research (Award 13-04-40405 comfi and 13-04-00049)
  • ERC
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2014-04-01
2024-04-24
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Sulfate-dependent acetate oxidation under extremely natron-alkaline conditions by syntrophic associations from hypersaline soda lakes
Microbiology 160, 723 (2014); https://doi.org/10.1099/mic.0.075093-0
/content/journal/micro/10.1099/mic.0.075093-0
/content/journal/micro/10.1099/mic.0.075093-0
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