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Volume 161, Issue 1

Genetic diversity of Shiga toxin-producing O157 : H7 recovered from human and food sources Free

Abstract

The aim of this study was to identify an epidemiological association between Shiga toxin-producing O157 : H7 strains associated with human infection and with food sources. Frequency distributions of different genetic markers of O157 : H7 strains recovered from human and food sources were compared using molecular assays to identify O157 : H7 genotypes associated with variation in pathogenic potential and host specificity. Genotypic characterization included: lineage-specific polymorphism assay (LSPA-6), clade typing, () polymorphism, Shiga toxin-encoding bacteriophage insertion site analysis and variant analysis of Shiga toxin 2 gene ( and ) and antiterminator genes ( and ). The intermediate lineage (LI/II) dominated among both food and human strains. Compared to other clades, clades 7 and 8 were more frequent among food and human strains, respectively. The () polymorphism occurred more frequently among human strains than food strains. and were found at significantly higher frequencies among food and human strains, respectively. Moreover, and were detected at significantly higher frequencies among human strains compared to food strains. Bivariate analysis revealed significant concordance (<0.05) between the LSPA-6 assay and the other typing methods. Multivariable regression analysis suggested that () was the most distinctive genotype that can be used to detect bacterial clones with potential risk for human illness from food sources. This study supported previous reports of the existence of diversity in genetic markers among different isolation sources by including O157 : H7 strains from both food and human sources. This might enable tracking genotypes with potential risk for human illness from food sources.

  • Received:
  • Accepted:
  • Published Online:
© 2015 The Authors
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2015-01-01
2024-04-19
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Genetic diversity of Shiga toxin-producing Escherichia coli O157 : H7 recovered from human and food sources
Microbiology 161, 112 (2015); https://doi.org/10.1099/mic.0.083063-0
/content/journal/micro/10.1099/mic.0.083063-0
/content/journal/micro/10.1099/mic.0.083063-0
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