1887

Abstract

Invasive infections caused by the important pathogen are often associated with disturbed blood coagulation in the human host, and may in severe cases develop into the life-threatening condition disseminated intravascular coagulation. In this study, the addition of M1 protein to human blood or purified peripheral blood mononuclear cells led to a dose-dependent increase of pro-coagulant activity, which was mediated by an upregulation of tissue factor on monocytes. Analysis of the resulting clots by transmission electron microscopy revealed that the cells were covered with a fibrin network that seemed to originate from the cell surface. Taken together, the results imply an important role for M proteins in the induction of haemostatic disorders in invasive streptococcal infectious diseases.

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2007-08-01
2024-03-29
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References

  1. Abraham E., Reinhart K., Opal S., Demeyer I., Doig C., Rodriguez A. L., Beale R., Svoboda P., Laterre P. F. other authors 2003; Efficacy and safety of tifacogin (recombinant tissue factor pathway inhibitor) in severe sepsis: a randomized controlled trial. JAMA 290:238–247
    [Google Scholar]
  2. Åkesson P., Schmidt K. H., Cooney J., Björck L. 1994; M1 protein and protein H: IgGFc- and albumin-binding streptococcal surface proteins encoded by adjacent genes. Biochem J 300:877–886
    [Google Scholar]
  3. Beres S. B., Richter E. W., Nagiec M. J., Sumby P., Porcella S. F., Deleo F. R., Musser J. M. 2006; Molecular genetic anatomy of inter- and intraserotype variation in the human bacterial pathogen group A Streptococcus . Proc Natl Acad Sci U S A 103:7059–7064
    [Google Scholar]
  4. Berge A., Björck L. 1995; Streptococcal cysteine proteinase releases biologically active fragments of streptococcal surface proteins. J Biol Chem 270:9862–9867
    [Google Scholar]
  5. Bisno A. L., Brito M. O., Collins C. M. 2003; Molecular basis of group A streptococcal virulence. Lancet Infect Dis 3:191–200
    [Google Scholar]
  6. Bryant A. E., Hayes-Schroer S. M., Stevens D. L. 2003; M type 1 and 3 group A streptococci stimulate tissue factor-mediated procoagulant activity in human monocytes and endothelial cells. Infect Immun 71:1903–1910
    [Google Scholar]
  7. Camerer E., Huang W., Coughlin S. R. 2000; Tissue factor- and factor X-dependent activation of protease-activated receptor 2 by factor VIIa. Proc Natl Acad Sci U S A 97:5255–5260
    [Google Scholar]
  8. Camerer E., Kataoka H., Kahn M., Lease K., Coughlin S. R. 2002; Genetic evidence that protease-activated receptors mediate factor Xa signaling in endothelial cells. J Biol Chem 277:16081–16087
    [Google Scholar]
  9. Carapetis J. R., Steer A. C., Mulholland E. K., Weber M. 2005; The global burden of group A streptococcal diseases. Lancet Infect Dis 5:685–694
    [Google Scholar]
  10. Collin M., Olsén A. 2000; Generation of a mature streptococcal cysteine proteinase is dependent on cell wall-anchored M1 protein. Mol Microbiol 36:1306–1318
    [Google Scholar]
  11. Creasey A. A., Chang A. C., Feigen L., Wun T. C., Taylor F. B. Jr, Hinshaw L. B. 1993; Tissue factor pathway inhibitor reduces mortality from Escherichia coli septic shock. J Clin Invest 91:2850–2860
    [Google Scholar]
  12. Cunningham M. A., Romas P., Hutchinson P., Holdsworth S. R., Tipping P. G. 1999; Tissue factor and factor VIIa receptor/ligand interactions induce proinflammatory effects in macrophages. Blood 94:3413–3420
    [Google Scholar]
  13. Cunningham M. W. 2000; Pathogenesis of group A streptococcal infections. Clin Microbiol Rev 13:470–511
    [Google Scholar]
  14. de Chateau M., Björck L. 1994; Protein PAB, a mosaic albumin-binding bacterial protein representing the first contemporary example of module shuffling. J Biol Chem 269:12147–12151
    [Google Scholar]
  15. Doshi S. N., Marmur J. D. 2002; Evolving role of tissue factor and its pathway inhibitor. Crit Care Med 30:S241–S250
    [Google Scholar]
  16. Fischetti V. A. 1989; Streptococcal M protein: molecular design and biological behavior. Clin Microbiol Rev 2:285–314
    [Google Scholar]
  17. Guha M., O'Connell M. A., Pawlinski R., Hollis A., McGovern P., Yan S. F., Stern D., Mackman N. 2001; Lipopolysaccharide activation of the MEK-ERK1/2 pathway in human monocytic cells mediates tissue factor and tumor necrosis factor alpha expression by inducing Elk-1 phosphorylation and Egr-1 expression. Blood 98:1429–1439
    [Google Scholar]
  18. Herwald H., Cramer H., Mörgelin M., Russell W., Sollenberg U., Norrby-Teglund A., Flodgaard H., Lindbom L., Björck L. 2004; M protein, a classical bacterial virulence determinant, forms complexes with fibrinogen that induce vascular leakage. Cell 116:367–379
    [Google Scholar]
  19. Kastern W., Sjöbring U., Björck L. 1992; Structure of peptostreptococcal protein L and identification of a repeated immunoglobulin light chain-binding domain. J Biol Chem 267:12820–12825
    [Google Scholar]
  20. Kihlberg B. M., Cooney J., Caparon M. G., Olsén A., Björck L. 1995; Biological properties of a Streptococcus pyogenes mutant generated by Tn 916 insertion in mga . Microb Pathog 19:299–315
    [Google Scholar]
  21. Kotb M., Norrby-Teglund A., McGeer A., El-Sherbini H., Dorak M. T., Khurshid A., Green K., Peeples J., Wade J. other authors 2002; An immunogenetic and molecular basis for differences in outcomes of invasive group A streptococcal infections. Nat Med 8:1398–1404
    [Google Scholar]
  22. Lancefield R. C. 1969; Current problems in studies of streptococci. J Gen Microbiol 55:161–163
    [Google Scholar]
  23. Levi M., de Jonge E., van der Poll T. 2003; Sepsis and disseminated intravascular coagulation. J Thromb Thrombolysis 16:43–47
    [Google Scholar]
  24. Loken M. R., Brosnan J. M., Bach B. A., Ault K. A. 1990; Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry. Cytometry 11:453–459
    [Google Scholar]
  25. Mackman N. 2006; Role of tissue factor in hemostasis and thrombosis. Blood Cells Mol Dis 36:104–107
    [Google Scholar]
  26. Opal S. M., Esmon C. T. 2003; Bench-to-bedside review: functional relationships between coagulation and the innate immune response and their respective roles in the pathogenesis of sepsis. Crit Care 7:23–38
    [Google Scholar]
  27. Påhlman L. I., Mörgelin M., Eckert J., Johansson L., Russell W., Riesbeck K., Soehnlein O., Lindbom L., Norrby-Teglund A. other authors 2006; Streptococcal M protein: a multipotent and powerful inducer of inflammation. J Immunol 177:1221–1228
    [Google Scholar]
  28. Pawlinski R., Pedersen B., Schabbauer G., Tencati M., Holscher T., Boisvert W., Andrade-Gordon P., Frank R. D., Mackman N. 2004; Role of tissue factor and protease-activated receptors in a mouse model of endotoxemia. Blood 103:1342–1347
    [Google Scholar]
  29. Price G. C., Thompson S. A., Kam P. C. 2004; Tissue factor and tissue factor pathway inhibitor. Anaesthesia 59:483–492
    [Google Scholar]
  30. Riedemann N. C., Guo R. F., Ward P. A. 2003; Novel strategies for the treatment of sepsis. Nat Med 9:517–524
    [Google Scholar]
  31. Roeder A., Kirschning C. J., Schaller M., Weindl G., Wagner H., Korting H. C., Rupec R. A. 2004; Induction of nuclear factor- κ B and c-Jun/activator protein-1 via Toll-like receptor 2 in macrophages by antimycotic-treated Candida albicans . J Infect Dis 190:1318–1326
    [Google Scholar]
  32. Stevens D. L. 1992; Invasive group A Streptococcus infections. Clin Infect Dis 14:2–11
    [Google Scholar]
  33. Stevens D. L. 2003; Group A streptococcal sepsis. Curr Infect Dis Rep 5:379–386
    [Google Scholar]
  34. Sutherland A. M., Walley K. R., Russell J. A. 2005; Polymorphisms in CD14, mannose-binding lectin, and Toll-like receptor-2 are associated with increased prevalence of infection in critically ill adults. Crit Care Med 33:638–644
    [Google Scholar]
  35. Taylor F. B., Chang A. C., Peer G., Li A., Ezban M., Hedner U. 1998; Active site inhibited factor VIIa (DEGR VIIa) attenuates the coagulant and interleukin-6 and -8, but not tumor necrosis factor, responses of the baboon to LD100 Escherichia coli . Blood 91:1609–1615
    [Google Scholar]
  36. Van Amersfoort E. S., Van Berkel T. J., Kuiper J. 2003; Receptors, mediators, and mechanisms involved in bacterial sepsis and septic shock. Clin Microbiol Rev 16:379–414
    [Google Scholar]
  37. Weineisen M., Sjöbring U., Fallman M., Andersson T. 2004; Streptococcal M5 protein prevents neutrophil phagocytosis by interfering with CD11b/CD18 receptor-mediated association and signaling. J Immunol 172:3798–3807
    [Google Scholar]
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