1887

Abstract

The phosphoenolpyruvate : carbohydrate phosphotransferase system (PTS) catalyses the transport of carbohydrates by coupling carbohydrate translocation and phosphorylation. In R, the genes and encode general components of the PTS, and genes and each encode fructose-, sucrose- and glucose-specific components of the PTS, respectively. In this study, we examined the mRNA levels of the genes in the presence or absence of PTS sugars. Glucose elevated the expression of and genes, whereas fructose and sucrose induced the expression of all the genes examined, i.e. - - - and -. We determined the transcriptional start sites of the genes and found that these promoters were activated in the presence of fructose. Disruption of , which is a deoxyribonucleoside repressor (DeoR)-type transcriptional regulator co-transcribed with , resulted in enhanced induction of the operon, , and expression in response to fructose, indicating that FruR attenuates the induction of , and by fructose.

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2008-01-01
2024-04-18
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