1887

Abstract

A mitochondrion-like organelle (MLO) was isolated from isotonic homogenates of . The organelle sedimented at 5000  for 10 min, and had an isopycnic density in sucrose of 1.2 g ml. Biochemical characterization enabled the demonstration of several key enzymes that allowed the construction of a metabolic pathway consisting of an incomplete Krebs cycle linked to the oxygen-sensitive enzymes pyruvate : NADP oxidoreductase (PNO), acetate : succinate CoA transferase (ASCT) and succinate thiokinase (STK), which cumulatively are responsible for recycling CoA and generating ATP. The organelle differs from typical aerobic mitochondria in possessing an oxygen-sensitive PNO that can use FAD or FMN as electron acceptor but is inactive with NAD, ferredoxin or ferredoxin. A gene with 77 % sequence similarity to the PNO mitochondrion precursor cluster from sp[Q941N5] was identified in the genome database. A second cluster with 56 % sequence similarity to the pyruvate : ferredoxin oxidoreductase (PFOR) from was also identified, which is in agreement with the concept that the PNO gene arose through the fusion of a eubacterial gene for PFOR with the gene for NADPH : cytochrome p450 reductase. Hydrogenase activity was not detected under the conditions used in this study. The oranelle therefore demonstrates significant biochemical differences from traditional mitochondria and hydrogenosomes, but possesses features of both. Based upon the results of this study, the organelle falls into the category of a MLO.

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2008-09-01
2024-03-28
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