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Volume 154, Issue 10

Development of an unambiguous and discriminatory multilocus sequence typing scheme for the group Free

Abstract

The zoonotic pathogen subsp. () is commonly found harmlessly colonizing the equine nasopharynx. Occasionally, strains can invade host tissues or cross species barriers, and is associated with numerous different diseases in a variety of hosts, including inflammatory airway disease and abortion in horses, pneumonia in dogs and meningitis in humans. A biovar of , subsp. , is the causative agent of strangles, one of the most important infections of horses worldwide. We report here the development of the first multilocus sequence typing (MLST) scheme for and its exploitation to define the population genetic structure of these related pathogens. A total of 130 unique sequence types were identified from 277 isolates of diverse geographical and temporal origin. Isolates of shared a recent evolutionary ancestor with isolates of that were significantly associated with cases of uterine infection or abortion in horses (<0.001). Isolates of from three UK outbreaks of acute fatal haemorrhagic pneumonia in dogs during 1999, 2001 and 2008 were found to be related to isolates from three outbreaks of this disease in the USA during 2005, 1993 and 2006, respectively. Our data provide strong evidence that evolved from an ancestral strain and that certain related strains of have a greater propensity to infect particular hosts and tissues.

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  • Accepted:
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  • Published Online:
Keyword(s): AHT, Animal Health Trust , DLV, double locus variant , dN/dS ratios, ratios of non-synonymous to synonymous substitutions , ET, electrophoretic type , ML, maximum-likelihood , MLEE, multi-enzyme electrophoresis , MLST, multilocus sequence typing , PFGE, pulsed-field gel electrophoresis , SLV, single locus variant , ST, sequence type and Δ–ln L, difference in log-likelihood
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2008-10-01
2024-04-19
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Development of an unambiguous and discriminatory multilocus sequence typing scheme for the Streptococcus zooepidemicus group
Microbiology 154, 3016 (2008); https://doi.org/10.1099/mic.0.2008/018911-0
/content/journal/micro/10.1099/mic.0.2008/018911-0
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