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Volume 149, Issue 7

The AIDA autotransporter adhesin recognizes an integral membrane glycoprotein as receptor Free

Abstract

The AIDA-I autotransporter adhesin, as a prototype of the AIDA adhesin family, represents a tripartite antigen consisting of the functional adhesin AIDA-I (-domain), which mediates the specific attachment of bacteria to target cells, and a two-domain translocator (AIDA) organized in the - and -domains. Cellular receptor moieties for the adhesin AIDA-I have not been identified. Here, it is demonstrated that the purified adhesin binds specifically to a high-affinity class of receptors on HeLa cells. Additionally, the adhesin was found to bind to a variety of mammalian cell types, indicating a broad tissue distribution of the receptor moiety. By using complementary techniques, including co-immunoprecipitation and one- and two-dimensional gel electrophoresis, the AIDA-I binding protein on HeLa cells was identified as a surface glycoprotein of about 119 kDa (gp119). The gp119 AIDA-I cellular receptor protein was characterized biochemically and found to be an integral -glycosylated membrane protein with a pI of 5·2.

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Keyword(s): AIDA, adhesin involved in diffuse adherence , CD, circular dichroism , GPI, glycosylphosphatidylinositol , HRP, horseradish peroxidase and PI-PLC, phosphatidylinositol-specific phospholipase C
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2003-07-01
2024-04-19
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The Escherichia coli AIDA autotransporter adhesin recognizes an integral membrane glycoprotein as receptor
Microbiology 149, 1871 (2003); https://doi.org/10.1099/mic.0.26264-0
/content/journal/micro/10.1099/mic.0.26264-0
/content/journal/micro/10.1099/mic.0.26264-0
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