1887

Microbiology Society logo

Volume 150, Issue 4

PsfR, a factor that stimulates expression in the cyanobacterium PCC 7942 Free

Abstract

In this paper a gene () is reported that regulates activity in , a unicellular photoautotrophic cyanobacterium that carries out oxygenic (plant-type) photosynthesis and exhibits global circadian regulation of gene expression. In , a family of three genes encodes the D1 protein of the photosystem II reaction centre. Overexpression of results in increased expression of , but does not affect the circadian timing of expression. overexpression affected some, but not all of the genes routinely surveyed for circadian expression. PsfR acts (directly or indirectly) on the basal promoter region. knockout mutants exhibit wild-type expression, suggesting that other factors can regulate expression in the absence of functional PsfR. PsfR contains two receiver-like domains (found in bacterial two-component signal transduction systems), one of which lacks the conserved aspartyl residue required for phosphoryl transfer. PsfR also contains a GGDEF domain. The presence of these domains and the absence of a detectable conserved DNA-binding domain suggest that PsfR may regulate expression via protein–protein interactions or GGDEF activity (the production of cyclic dinucleotides) rather than direct interaction with the promoter.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.26915-0
2004-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/150/4/mic1501031.html?itemId=/content/journal/micro/10.1099/mic.0.26915-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
     [Google Scholar]
  2. Andersson C. R., Tsinoremas N. F., Shelton J., Lebedeva N. V., Yarrow J., Min H., Golden S. S. 2000; Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Methods Enzymol 305:527–542
     [Google Scholar]
  3. Ausmees N., Mayer R., Weinhouse H., Volman G., Amikam D., Benziman M., Lindberg M. 2001; Genetic data indicate that proteins containing the GGDEF domain possess diguanylate cyclase activity. FEMS Microbiol Lett 204:163–167 [CrossRef]
     [Google Scholar]
  4. Bustos S. A., Golden S. S. 1991; Expression of the psbDII gene in Synechococcus sp. strain PCC 7942 requires sequences downstream of the transcription start site. J Bacteriol 173:7525–7533
     [Google Scholar]
  5. Elledge S. J., Davis R. W. 1989; Position and density effects on repression by stationary and mobile DNA-binding proteins. Genes Dev 3:185–197 [CrossRef]
     [Google Scholar]
  6. Golden J. W., Wiest D. R. 1988; Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene. Science 242:1421–1423 [CrossRef]
     [Google Scholar]
  7. Golden S. S., Stearns G. W. 1988; Nucleotide sequence and transcript analysis of three photosystem II genes from the cyanobacterium Synechococcus. sp PCC7942 Gene 67:85–96 [CrossRef]
     [Google Scholar]
  8. Golden S. S., Brusslan J., Haselkorn R. 1986; Expression of a family of psbA genes encoding a photosystem II polypeptide in the cyanobacterium Anacystis nidulans R2. EMBO J 5:2789–2798
     [Google Scholar]
  9. Golden S. S., Brusslan J., Haselkorn R. 1987; Genetic engineering of the cyanobacterial chromosome. Methods Enzymol 153:215–231
     [Google Scholar]
  10. Golden S. S., Ishiura M., Johnson C. H., Kondo T. 1997; Cyanobacterial circadian rhythms. Annu Rev Plant Physiol Plant Mol Biol 48:327–354 [CrossRef]
     [Google Scholar]
  11. Imamura A., Hanaki N., Nakamura A., Suzuki T., Taniguchi M., Kiba T., Ueguchi C., Sugiyama T., Mizuno T. 1999; Compilation and characterization of Arabidopsis thaliana response regulators implicated in His-Asp phosphorelay signal transduction. Plant Cell Physiol 40:733–742 [CrossRef]
     [Google Scholar]
  12. Ishiura M., Kutsuna S., Aoki S., Iwasaki H., Andersson C. R., Tanabe A., Golden S. S., Johnson C. H., Kondo T. 1998; Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria. Science 281:1519–1523 [CrossRef]
     [Google Scholar]
  13. Katayama M., Tsinoremas N. F., Kondo T., Golden S. S. 1999; cpmA, a gene involved in an output pathway of the cyanobacterial circadian system. J Bacteriol 181:3516–3524
     [Google Scholar]
  14. Katayama M., Kondo T., Xiong J., Golden S. S. 2003; ldpA encodes an iron-sulfur protein involved in light-dependent modulation of the circadian period in the cyanobacterium Synechococcus elongatus PCC 7942. J Bacteriol 185:1415–1422 [CrossRef]
     [Google Scholar]
  15. Kondo T., Strayer C. A., Kulkarni R. D., Taylor W., Ishiura M., Golden S. S., Johnson C. H. 1993; Circadian rhythms in prokaryotes: luciferase as a reporter of circadian gene expression in cyanobacteria. Proc Natl Acad Sci U S A 90:5672–5676 [CrossRef]
     [Google Scholar]
  16. Kondo T., Tsinoremas N. F., Golden S. S., Johnson C. H., Kutsuna S., Ishiura M. 1994; Circadian clock mutants of cyanobacteria. Science 266:1233–1236 [CrossRef]
     [Google Scholar]
  17. Liu Y., Tsinoremas N. F., Johnson C. H., Lebedeva N. V., Golden S. S., Ishiura M., Kondo T. 1995; Circadian orchestration of gene expression in cyanobacteria. Genes Dev 9:1469–1478 [CrossRef]
     [Google Scholar]
  18. Liu Y., Tsinoremas N. F., Golden S. S., Kondo T., Johnson C. H. 1996; Circadian expression of genes involved in the purine biosynthetic pathway of the cyanobacterium Synechococcus sp. strain PCC 7942. Mol Microbiol 20:1071–1081 [CrossRef]
     [Google Scholar]
  19. Makino S., Kiba T., Imamura A.7 other authors 2000; Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana. Plant Cell Physiol 41:791–803 [CrossRef]
     [Google Scholar]
  20. Marsh J. L., Erfle M., Wykes E. J. 1984; The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481–485 [CrossRef]
     [Google Scholar]
  21. Nair U., Thomas C., Golden S. S. 2001; Functional elements of the strong psbAI promoter of Synechococcus elongatus PCC 7942. J Bacteriol 183:1740–1747 [CrossRef]
     [Google Scholar]
  22. Nair U., Ditty J. L., Min H., Golden S. S. 2002; Roles for sigma factors in global circadian regulation of the cyanobacterial genome. J Bacteriol 184:3530–3538 [CrossRef]
     [Google Scholar]
  23. O'Hara B. P., Norman R. A., Wan P. T., Roe S. M., Barrett T. E., Drew R. E., Pearl L. H. 1999; Crystal structure and induction mechanism of AmiC-AmiR: a ligand-regulated transcription antitermination complex. EMBO J 18:5175–5186 [CrossRef]
     [Google Scholar]
  24. Pei J., Grishin N. V. 2001; GGDEF domain is homologous to adenylyl cyclase. Proteins 42:210–216 [CrossRef]
     [Google Scholar]
  25. Sambrook J., Russell D. W. 2001 Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  26. Schaefer M. R., Golden S. S. 1989; Light availability influences the ratio of two forms of D1 in cyanobacterial thylakoids. J Biol Chem 264:7412–7417
     [Google Scholar]
  27. Schmitz O., Katayama M., Williams S. B., Kondo T., Golden S. S. 2000; CikA, a bacteriophytochrome that resets the cyanobacterial circadian clock. Science 289:765–768 [CrossRef]
     [Google Scholar]
  28. Stock A. M., Robinson V. L., Goudreau P. N. 2000; Two-component signal transduction. Annu Rev Biochem 69:183–215 [CrossRef]
     [Google Scholar]
  29. Strayer C., Oyama T., Schultz T. F., Raman R., Somers D. E., Mas P., Panda S., Kreps J. A., Kay S. A. 2000; Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog. Science 289:768–771 [CrossRef]
     [Google Scholar]
  30. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268 [CrossRef]
     [Google Scholar]
  31. Williams S. B., Vakonakis I., Golden S. S., LiWang A. C. 2002; Structure and function from the circadian clock protein KaiA of Synechococcus elongatus: a potential clock input mechanism. Proc Natl Acad Sci U S A 99:15357–15362 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.26915-0
Loading
PsfR, a factor that stimulates psbAI expression in the cyanobacterium Synechococcus elongatus PCC 7942
Microbiology 150, 1031 (2004); https://doi.org/10.1099/mic.0.26915-0
/content/journal/micro/10.1099/mic.0.26915-0
/content/journal/micro/10.1099/mic.0.26915-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error