1887

Abstract

The AbrB protein is a DNA-binding global regulator of a plethora of functions that are expressed during the transition from exponential growth to stationary phase and under suboptimal growth conditions. AbrB orthologues have been identified in a variety of prokaryotic organisms, notably in all species of , and that have been examined. Based on amino acid sequence identity in the N-terminal domains of the orthologues from and , it was predicted that the proteins might display identical DNA-binding specificities. The binding of purified AbrB (AbrB) and purified AbrB (AbrB) at DNA targets of , and a synthetic origin was compared. In all cases examined, DNA-binding specificity was identical as judged by DNase I footprinting. In cells, the promoters from the and genes were regulated by AbrB, and the promoter from the operon was regulated by AbrB.

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2005-06-01
2024-03-29
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Baillie L., Moir A., Manchee R. 1998; The expression of the protective antigen of Bacillus anthracis in Bacillus subtilis. J Appl Microbiol 84:741–746 [CrossRef]
    [Google Scholar]
  3. Crothers D. M., Haran T. E., Nadeau J. G. 1990; Intrinsically bent DNA. J Biol Chem 265:7093–7096
    [Google Scholar]
  4. Dai Z., Sirard J. C., Mock M., Koehler T. M. 1995; The atxA gene product activates transcription of the anthrax toxin genes and is essential for virulence. Mol Microbiol 16:1171–1181 [CrossRef]
    [Google Scholar]
  5. Ferrari E., Howard S. M., Hoch J. A. 1986; Effect of stage 0 sporulation mutations on subtilisin expression. J Bacteriol 166:173–179
    [Google Scholar]
  6. Guerout-Fleury A. M., Frandsen N., Stragier P. 1996; Plasmids for ectopic integration in Bacillus subtilis. Gene 180:57–61 [CrossRef]
    [Google Scholar]
  7. Hamon M. A., Stanley N. R., Britton R. A., Grossman A. D., Lazazzera B. A. 2004; Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis. Mol Microbiol 52:847–860 [CrossRef]
    [Google Scholar]
  8. Hoch J. A. 1991; Genetic analysis in Bacillus subtilis. Methods Enzymol 204:305–320
    [Google Scholar]
  9. Ireton K., Rudner D. Z., Siranosian K. J., Grossman A. D. 1993; Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor. Genes Dev 7:283–294 [CrossRef]
    [Google Scholar]
  10. Molle V., Fujita M., Jensen S. T., Eichenberger P., Gonzalez-Pastor J. E., Liu J. S., Losick R. 2003; The Spo0A regulon of Bacillus subtilis. Mol Microbiol 50:1683–1701 [CrossRef]
    [Google Scholar]
  11. Perego M. 1993; Integrational vectors for genetic manipulation in Bacillus subtilis. In Bacillus subtilis and Other Gram Positive Bacteria: Biochemistry, Physiology and Molecular Genetics pp 615–624 Edited by Sonenhein A. L., Hoch J. A., Losick R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Perego M., Spiegelman G. B., Hoch J. A. 1988; Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis. Mol Microbiol 2:689–699 [CrossRef]
    [Google Scholar]
  13. Phillips Z. E., Strauch M. A. 2002; Bacillus subtilis sporulation and stationary phase gene expression. Cell Mol Life Sci 59:392–402 [CrossRef]
    [Google Scholar]
  14. Saile E., Koehler T. M. 2002; Control of anthrax toxin gene expression by the transition state regulator abrB. J Bacteriol 184:370–380 [CrossRef]
    [Google Scholar]
  15. Shimotsu H., Henner D. J. 1986; Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis. Gene 43:85–94 [CrossRef]
    [Google Scholar]
  16. Strauch M. A. 1993; Regulation of Bacillus subtilis gene expression during the transition from exponential growth to stationary phase. Prog Nucleic Acid Res Mol Biol 46:121–153
    [Google Scholar]
  17. Strauch M. A. 1995a; In vitro binding affinity of the Bacillus subtilis AbrB protein to six different DNA target regions. J Bacteriol 177:4532–4536
    [Google Scholar]
  18. Strauch M. A. 1995b; AbrB modulates expression and catabolite repression of a Bacillus subtilis ribose transport operon. J Bacteriol 177:6727–6731
    [Google Scholar]
  19. Strauch M. A. 1996; Dissection of the Bacillus subtilis spoOE binding site for the global regulator AbrB reveals smaller recognition elements. Mol Gen Genet 250:742–749
    [Google Scholar]
  20. Strauch M. A., Hoch J. A. 1993; Transition-state regulators: sentinels of Bacillus subtilis post-exponential gene expression. Mol Microbiol 7:337–342 [CrossRef]
    [Google Scholar]
  21. Strauch M. A., Perego M., Burbulys D., Hoch J. A. 1989a; The transition state transcription regulator AbrB of Bacillus subtilis is autoregulated during vegetative growth. Mol Microbiol 3:1203–1209 [CrossRef]
    [Google Scholar]
  22. Strauch M. A., Spiegelman G. B., Perego M., Johnson W. C., Burbulys D., Hoch J. A. 1989b; The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein. EMBO J 8:1615–1621
    [Google Scholar]
  23. Strauch M., Webb V., Spiegelman G., Hoch J. A. 1990; The SpoOA protein of Bacillus subtilis is a repressor of the abrB gene. Proc Natl Acad Sci U S A 87:1801–1805 [CrossRef]
    [Google Scholar]
  24. Vaughn J. L., Feher V., Naylor S., Strauch M. A., Cavanagh J. 2000; Novel DNA binding domain and genetic regulation model of Bacillus subtilis transition state regulator AbrB. Nat Struct Biol 7:1139–1146 [CrossRef]
    [Google Scholar]
  25. Xu K., Strauch M. A. 1996; In vitro selection of optimal AbrB-binding sites: comparison to known in vivo sites indicates flexibility in AbrB binding and recognition of three-dimensional DNA structures. Mol Microbiol 19:145–158 [CrossRef]
    [Google Scholar]
  26. Xu K., Strauch M. A. 2001; DNA-binding activity of amino-terminal domains of the Bacillus subtilis AbrB protein. J Bacteriol 183:4094–4098 [CrossRef]
    [Google Scholar]
  27. Xu K., Clark D., Strauch M. A. 1996; Analysis of abrB mutations, mutant proteins, and why abrB does not utilize a perfect consensus in the −35 region of its sigma A promoter. J Biol Chem 271:2621–2626 [CrossRef]
    [Google Scholar]
  28. Yoshida K., Seki S., Fujimura M., Miwa Y., Fujita Y. 1995; Cloning and sequencing of a 36-kb region of the Bacillus subtilis genome between the gnt and iol operons. DNA Res 2:61–69 [CrossRef]
    [Google Scholar]
  29. Yoshida K., Fujita Y., Ehrlich S. D. 1999; Three asparagine synthetase genes of Bacillus subtilis. J Bacteriol 181:6081–6091
    [Google Scholar]
  30. Zuber P. 2000; Specificity through flexibility. Nat Struct Biol 7:1079–1081 [CrossRef]
    [Google Scholar]
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