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Volume 151, Issue 12

Overall control of nitrogen metabolism in by CodY, and possible models for CodY regulation in Firmicutes Free

Abstract

CodY, a pleiotropic transcriptional regulator conserved in low G+C species of Gram-positive bacteria, was previously described to be the central regulator of proteolysis in . In this study, over 100 potential CodY targets were identified by DNA-microarray analysis. Complementary transcriptional analysis experiments were carried out to validate the newly defined CodY regulon. Moreover, the direct role of CodY in the regulation of several target genes was demonstrated by gel retardation experiments. Interestingly, 45 % of CodY-dependent genes encode enzymes involved in amino acid biosynthesis pathways, while most of the other genes are involved in functions related to nitrogen supply. CodY of represents the first example of a regulator in Gram-positive bacteria that globally controls amino acid biosynthesis. This global control leads to growth inhibition in several amino-acid-limited media containing an excess of isoleucine. A conserved 15 nt palindromic sequence (AATTTTCNGAAAATT), the so-called CodY-box, located in the vicinity of the −35 box of target promoter regions was identified. Relevance of the CodY-box as an operator for CodY was demonstrated by base substitutions in gel retardation experiments. This motif is also frequently found in the promoter region of genes potentially regulated by CodY in other Gram-positive bacteria.

  • Received:
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  • Published Online:
Keyword(s): BCAA, branched-chain amino acid(s) and RT-QPCR, real-time quantitative PCR
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2005-12-01
2024-04-19
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Overall control of nitrogen metabolism in Lactococcus lactis by CodY, and possible models for CodY regulation in Firmicutes
Microbiology 151, 3895 (2005); https://doi.org/10.1099/mic.0.28186-0
/content/journal/micro/10.1099/mic.0.28186-0
/content/journal/micro/10.1099/mic.0.28186-0
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