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Volume 152, Issue 9

Implication of (Mn)superoxide dismutase of in oxidative stress responses and survival inside macrophages Free

Abstract

The gene encoding the manganese-containing superoxide dismutase (MnSOD) of was characterized. It is transcribed monocistronically from an upstream promoter identified by rapid amplification of cDNA ends (RACE)-PCR. A mutant was constructed and characterized. Growth of the mutant strain was not significantly different from that of its wild-type counterpart in standing and aerated cultures. However, the mutant was more sensitive towards menadione and hydroperoxide stresses. The response to HO stress was analysed in more detail, and the mode of killing of this oxidant was different under anaerobic and aerobic conditions. Cultures grown and challenged under anaerobic conditions were highly sensitive to treatment with 35 mM HO. They were largely protected by the iron chelator deferoxamine, which suggested that killing was mainly due to an enhanced Fenton reaction. In contrast, neither strain was protected by the iron chelators deferoxamine and diethylenetriaminepentaacteic acid when grown and challenged under aerobic conditions, which suggested that inactivation of the cells by HO was due to another killing mode. The mutant was more sensitive under these conditions, showing that MnSOD is also important for protecting the cells from damage under aerobic conditions. Finally, the MnSOD of may be considered to be a virulence factor, since survival of the corresponding mutant strain was highly affected inside mouse peritoneal macrophages.

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Keyword(s): Cm, chloramphenicol , DAPE, diaminopimelate epimerase , Em, erythromycin , MnSOD, manganese-containing superoxide dismutase , RACE-PCR, rapid amplification of cDNA ends PCR , ROS, reactive oxygen species , SOD, superoxide dismutase and tBOOH, tert-butylhydroperoxide
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2006-09-01
2024-04-20
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Implication of (Mn)superoxide dismutase of Enterococcus faecalis in oxidative stress responses and survival inside macrophages
Microbiology 152, 2579 (2006); https://doi.org/10.1099/mic.0.28922-0
/content/journal/micro/10.1099/mic.0.28922-0
/content/journal/micro/10.1099/mic.0.28922-0
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