1887

Abstract

SUMMARY: While lomofungin at 20 μg ml effectively halted the synthesis of ribonucleic acids in protoplasts of , the onset of biogenesis of new wall on the protoplast surface was not impaired. The synthesis of mannan-protein wall matrix continued for about 30 min after addition of lomofungin while the formation of 1,3-β-glucan networks was not influenced even after prolonged incubation. These results suggest that the life-span of limiting mRNA molecules coding for the proteins of the wall matrix is about 30 min while the turnover of 1,3-β-glucan synthases is relatively low.

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1979-02-01
2024-04-26
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References

  1. Cannon M., Davies J. E., Jimenez A. 1973; Inhibition by lomofungin of nucleic acid and protein synthesis in Saccharomyces cerevisiae. FEBS Letters 32:277–280
    [Google Scholar]
  2. Cano F. R., Kuo S. C., Lampen J. O. 1973; Lomofungin, an inhibitor of deoxyribonucleic acid-dependent ribonucleic acid polymerases. Antimicrobial Agents and Chemotherapy 3:723–728
    [Google Scholar]
  3. Eddy A. A., Williamson D. H. 1959; Formation of aberrant cell wall and of spores by the growing yeast protoplasts. Nature, London 183:1101–1104
    [Google Scholar]
  4. Elorza M. V., Lostau C. M., Villanueva J. R., Sentandreu R. 1976; Cell wall synthesis regulation in Saccharomyces cerevisiae. Effect of RNA and protein inhibition. Biochimica et biophysica acta 454:263–272
    [Google Scholar]
  5. Farkaš V., Svoboda A., Bauer Š. 1969; Inhibitory effect of 2-deoxy-d-glucose on the formation of the cell wall in yeast protoplasts. Journal of Bacteriology 98:744–748
    [Google Scholar]
  6. Farkaš V., Svoboda A., Bauer Š. 1970; Secretion of cell wall glycoproteins by yeast protoplasts. Effect of 2-deoxy-d-glucose and cycloheximide. Biochemical Journal 118:755–758
    [Google Scholar]
  7. Farkaš V., Biely P., Bauer Š. 1973; Extracellular β-glucanases of the yeast, Saccharomyces cerevisiae. Biochimica et biophysica acta 321:246–255
    [Google Scholar]
  8. Fraser R. S. S., Creanor J., Mitchison J. M. 1973; Rapid and selective inhibition of the synthesis of high molecular weight RNA in yeast by lomofungin. Nature, London 244:222–224
    [Google Scholar]
  9. Hutchinson H. T., Hartwell L. H., McLauchin C. S. 1969; Temperature sensitive yeast mutant defective in ribonucleic acid production. Journal of Bacteriology 99:807–814
    [Google Scholar]
  10. Johnson B. F. 1967; Growth of the fission yeast, Schizosaccharomyces pombe, with late, excentric lytic fission in an unbalanced medium. Journal of Bacteriology 94:192–195
    [Google Scholar]
  11. Kelleti T., Szabolzi G., Lenvai A., Garzo T. 1954; Untersuchungen fiber die lebens fähligen Eiweisskörper von Saccharomyces cerevisiae. Die Regeneration in sterilen Filtrat von zerstörn Hefezellen. Acta scientifica hungarica 5:213–217
    [Google Scholar]
  12. Klebs G. 1887; Über den Einfluss des Kernes in der Zelle. Biologisches Zentralblatt 7:161
    [Google Scholar]
  13. Klebs G. 1888; Beiträge zur Physiologie der Pflanzenzelle. Untersuchungen den Botanisches Institut Tübingen 2:489
    [Google Scholar]
  14. Kopecká M. 1976a Biogenesis of the fibrillar wall component in yeast protoplasts Ph. D. thesis, J. E. Purkynĕ University, Brno, Czechoslovakia;
    [Google Scholar]
  15. Kopecká M. 1976b; The use of the antibiotic lomofungin for demonstration of nuclei and chromosomes in live yeast cells and protoplasts. Folia microbiologica 21:406–408
    [Google Scholar]
  16. Kopecká M., Gabriel M. 1978; Staining the nuclei in cells and protoplasts of living yeasts, moulds and green algae with the antibiotic lomofungin. Archives of Microbiology (in the Press)
    [Google Scholar]
  17. Kopecká M., Čtvrtníček O., Nečas O. 1967; Formation and properties of fibrillar network formed in yeast protoplasts as the first step of biosynthesis of the cell wall.. In Symposium über Hefe-Protoplasten, pp. 73–75 Edited by Müller R. Berlin: Akademie-Verlag;
    [Google Scholar]
  18. Kopecká M., Svoboda A., Nečas O. 1968; New findings on incipient biosynthesis of the cell wall in yeast protoplasts. Folia microbiologica 13:526
    [Google Scholar]
  19. Kopecká M., Nečas O., Svoboda A. 1970; Structure and formation of the fibrillar component of the growing wall in yeast protoplasts. Proceedings of the Second International Symposium on Yeast Protoplasts, Brno J. E. Purkynĕ University, pp. 197–202 Edited by Nečas O., Svoboda A. Brno, Czechoslovakia.: Acta Facultatis Medicae Universitatis Brunensis;
    [Google Scholar]
  20. Kopecká M., Gabriel M., Nečas O. 1974; A method of isolating anucleated yeast protoplasts unable to synthesize the glucan fibrillar component of the cell wall. Journal of General Microbiology 81:111–120
    [Google Scholar]
  21. Kreger D. R., Kopecká M. 1973; On the nature of the fibrillar nets formed by protoplasts of Saccharomyces cerevisiae in liquid media.. In Yeast, Mould and Plant Protoplasts, pp. 117–130 Edited by Villaneuva J. R., Garcia-Acha I., Gascón S., Uruburu F. London: Academic Press;
    [Google Scholar]
  22. Kreger D. R., Kopecká M. 1976a; On the nature and formation of the fibrillar nets produced by protoplasts of Saccharomyces cerevisiae in liquid media: an electronmicroscopic, X-ray diffraction and chemical study. Journal of General Microbiology 92:207–220
    [Google Scholar]
  23. Kreger D. R., Kopecká M. 1976b; Assembly of wall polymers during the regeneration of yeast protoplasts.. In Microbial and Plant Protoplasts, pp. 237–252 Edited by Peberdy J. F., Rose A. H., Rogers H. J., Cocking E. C. London: Academic Press;
    [Google Scholar]
  24. Kuo S. C., Lampen J. O. 1974; Tunicamycin, an inhibitor of yeast glycoprotein synthesis. Biochemical and Biophysical Research Communications 58:287–295
    [Google Scholar]
  25. Kuo S. C., Cano F. R., Lampen J. O. 1973; Lomofungin, an inhibitor of ribonucleic acid synthesis in yeast protoplasts: its effect on enzyme formation. Antimicrobial Agents and Chemotherapy 3:716–722
    [Google Scholar]
  26. Lampen J. O. 1968; External enzymes of yeast: their nature and formation. Antonie van Leeuwenhoek 34:1–18
    [Google Scholar]
  27. Nečas O. 1956; Žiznesposobnost kletočnych fragmentov drožžej. IV. Svjaz meždu jadrom i sposobnostju k rostu. Folia biologica 2:29–31
    [Google Scholar]
  28. Nečas O. 1961; Physical conditions as important factors for the regeneration of naked yeast protoplasts. Nature, London 192:580–581
    [Google Scholar]
  29. Nečas O. 1965; The mechanism of regeneration of yeast protoplasts. II. Formation of the cell wall de novo. Folia biologica 11:97–102
    [Google Scholar]
  30. Nečas O. 1971; Cell wall synthesis in yeast protoplasts. Bacteriological Reviews 35:149–170
    [Google Scholar]
  31. Nečas O., Svoboda A. 1967; Mechanism of regeneration of yeast protoplasts. IV. Electron microscopy of regenerating protoplasts. Folia biologica 13:379–385
    [Google Scholar]
  32. Nečas O., Svoboda A., Kopecká M. 1968; The effect of cycloheximide (actidione) on cell wall synthesis in yeast protoplasts. Experimental Cell Research 53:291–293
    [Google Scholar]
  33. Ruet A., Bouhet J. C., Buhler J. M., Senteneac A. 1975; On the mode of action of lomofungin, an inhibitor of RNA synthesis in yeast. Biochemistry 14:4651–4658
    [Google Scholar]
  34. Sachs L. 1882 Vorlesungen über Pflanzen-Physiologie Leipzig;
    [Google Scholar]
  35. Sierra J. M., Sentandreu R., Villanueva J. R. 1973; Regulation of wall synthesis during Saccharomyces cerevisiae cell cycle. FEBS Letters 34:285–290
    [Google Scholar]
  36. Sošková L., Soška J., Svoboda A. 1970; The effect of 5-fluorouracil on cells and regenerating protoplasts of Saccharomyces cerevisiae. Folia microbiologica 15:442–447
    [Google Scholar]
  37. Surdin-Kerjan Y., Robichon-Szulmajster H. 1975; Existence of two levels of repression in the biosynthesis of methionine in Saccharomyces cerevisiae: effect of lomofungin in enzyme synthesis. Journal of Bacteriology 122:365–374
    [Google Scholar]
  38. Svoboda A. 1966; Regeneration of yeast protoplasts in agar cells. Experimental Cell Research 44:640–642
    [Google Scholar]
  39. Svoboda A., Nečas O. 1970; Experimental decoupling in the synthesis of fibrillar and amorphous components during regeneration of the cell wall in Saccharomyces cerevisiae protoplasts. Proceedings of the Second International Symposium on Yeast Protoplasts, Brno J. E. Purkyne University, pp. 211–215 Edited by Nečas O., Svoboda A. Brno, Czechoslovakia: Acta Facultatis Medicae Universitatis Brunensis;
    [Google Scholar]
  40. Tønnesen T., Friesen J. D. 1973; Inhibitors of ribonucleic acid synthesis in Saccharomyces cerevisiae: decay rate of messenger ribonucleic acid. Journal of Bacteriology 115:889–896
    [Google Scholar]
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