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Separation on Urografin Gradients of Subpopulations from Sporulating Saccharomyces cerevisiae Cultures
Abstract
Sporulating yeast populations were separated into a number of fractions by centrifugation through linear density gradients of Urografin. At least eight distinct fractions could be obtained from cultures that were just beginning to produce asci visible by phase contrast microscopy. Cells in these fractions were found to differ in the stage they had reached in meiosis and sporulation; those more advanced in sporulation banded at a lower density. Using this gradient centrifugation method it was shown that cultures induced to sporulate by the usual technique were markedly heterogeneous with respect to the stage in meiosis and sporulation reached by the cells, and that most of the labelled amino acids incorporated into protein by sporulating cultures appeared in those cells not yet committed to either recombination or meiosis.
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© Society for General Microbiology, 1980