1887

Abstract

The protonmotive force, Na/H antiport activity and a-aminoisobutyric acid transport were studied in alkaline-tolerant ATCC 14575 and in a newly isolated alkalophilic strain of the same species. The alkalophilic strain, which grew over a pH range from 8·0 to 10·5, maintained a cytoplasmic pH of 9·5 or below even at an external pH of 110. Evidence was obtained for an electrogenic Na/H antiporter which could play a role in acidification of the cytoplasm as well as in generation of the transmembrane electrical potential. A non-alkalophilic strain, isolated after mutagenesis of the new alkalophile, lacked the Na/H antiporter. The alkaline-tolerant strain of , which grew at pH values between 6·5 and 8·5, did not maintain an acidified cytoplasm as the pH was raised above 8·0. A less active, apparently electroneutral, Na/H exchange was found in this strain. Both the alkaline-tolerant and alkalophilic strains exhibited Na-dependent transport of -aminoisobutyric acid.

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1980-07-01
2024-04-18
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References

  1. Bhattacharyya P., Barnes E. M.Jr 1978; Proton-coupled sodium uptake by membrane vesicles from Azotobacter vinelandii.. Journal of Biological Chemistry 253:3848–3851
    [Google Scholar]
  2. Brey R. N., Rosen B. P. 1979; Cation/proton antiport systems in Escherichia coli. . Journal of Biological Chemistry 254:1957–1963
    [Google Scholar]
  3. Cole J., Wimpenny J. W. T., Hughes D. E. 1967; The ATP pool in Escherichia coli. I. Measurement of the pool using a modified luciferase assay.. Biochimica et biophysica acta 143:445–453
    [Google Scholar]
  4. Gordon R. E. 1980; 107 years of the genus Bacillus.. In The Aerobic Endospore-forming Bacteria: Classification and Identification (in the Press). Berkeley R. C. W., Good-fellow M. Edited by London:: Academic Press.;
    [Google Scholar]
  5. Guffanti A. A., Susman P., Blanco R., Krulwich T. A. 1978; The protonmotive force and α-aminoisobutyric acid transport in an obligately alkalophilic bacterium.. Journal of Biological Chemistry 253:708–715
    [Google Scholar]
  6. Guffanti A. A., Blanco R., Krulwich T. A. 1979a; A requirement for ATP for β-galactoside transport by Bacillus alcalophilus.. Journal of Biological Chemistry 254:1033–1037
    [Google Scholar]
  7. Guffanti A. A., Monti L., Blanco R., Ozick D., Krulwich T. A. 1979b; β-Galactoside transport in an alkaline-tolerant strain of Bacillus circulans.. Journal of General Microbiology 112:161–169
    [Google Scholar]
  8. Harold F. M., Papineau D. 1972; Cation transport and electrogenesis by Streptococcus faecalis. II. Proton and sodium extrusion.. Journal of Membrane Biology 8:45–62
    [Google Scholar]
  9. Hegeman G. D. 1966; Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type.. Journal of Bacteriology 91:1140–1154
    [Google Scholar]
  10. Lanyi J. K., MacDonald R. E. 1976; Existence of electrogenic hydrogen ion/sodium ion antiport in Halobacterium halobium cell envelope vesicles.. Biochemistry 15:4608–4614
    [Google Scholar]
  11. Mandel K. G., Krulwich T. A. 1979; Monovalent cation-proton antiports in membrane vesicles of Bacillus alcalophilus.. Abstracts of Xlth International Congress of Biochemistry Toronto, Canada: p. 449
    [Google Scholar]
  12. Mitchell P. 1961; Coupling of phosphorylation to electron and hydrogen transfer by a chemiosmo- tic type of mechanism.. Nature London:191141–148
    [Google Scholar]
  13. Niven D. F., MacLeod R. A. 1978; Sodium ion- proton antiport in a marine bacterium.. Journal of Bacteriology 134:737–743
    [Google Scholar]
  14. Padan E., Zilberstein D., Rottenberg H. 1976; The proton electrochemical gradient in Escherichia coli cells.. European Journal of Biochemistry 63:533–541
    [Google Scholar]
  15. Pillay D. T. N., Mehdi R. 1970; Separation of amino acids by thin-layer chromatography.. Journal of Chromatography 47:119–123
    [Google Scholar]
  16. Ramos S., Kaback H. R. 1977; The electrochemical proton gradient in Escherichia colimembrane vesicles.. Biochemistry 16:848–854
    [Google Scholar]
  17. Ramos S., Schuldiner S., Kaback H. R. 1976; The electrical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles.. Proceedings of the National Academy of Sciences of the United States of America 73:1892–1896
    [Google Scholar]
  18. Schulachev V. P. 1978; Membrane linked energy buffering as the biological function of Na+/K+ gradient.. FEBS Letters 87:171–179
    [Google Scholar]
  19. Schuldiner S., Fishkes H. 1978; Sodium- proton antiport in isolated membrane vesicles of Escherichia coli.. Biochemistry 17:706–711
    [Google Scholar]
  20. Schuldiner S., Kaback H. R. 1975; Membrane potential and active transport in membrane vesicles from Escherichia coli.. Biochemistry 14:5451–5461
    [Google Scholar]
  21. Stanley P. E., Williams S. G. 1969; Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme.. Analytical Biochemistry 29:381–392
    [Google Scholar]
  22. Stock J. B., Rauch B., Roseman S. 1977; Periplasmic space in Salmonella typhimurium and Escherichia coli.. Journal of Biological Chemistry 252:7850–7861
    [Google Scholar]
  23. Thompson J. 1976; Characteristics and energy requirements of an α-aminoisobutyrie acid transport system in Streptococcus lactis.. Journal of Bacteriology 127:719–730
    [Google Scholar]
  24. Wolfson P. J., Krulwich T. A. 1972; Inhibition of isocitrate lyase: the basis for inhibition of growth of two Arthrobacter species by pyruvate.. Journal of Bacteriology 112:356–364
    [Google Scholar]
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