1887

Abstract

Evidence was sought for gene-specific negative regulatory element of similar to the repressor/operator systems that control some operons in rpkaryotes and their viruses. Mutants of that constitutively express the stage specific enzyme α-mannosidase-2 could provide such evidence. Reconstruction experiments demonstrated that the screening technique developed would detect mutants of that type. Over 2 × 10 surviovors of heavily mutagenized cell populations were grown and their progeny tested. No mutants of the desired type were obtained. The possible implications of this finding are discussed.

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1980-09-01
2024-05-18
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References

  1. Alton T., Lodish H. 1977; Developmental changes in mRNA and protein synthesis in Dictyostelium discoideum. . Developmental Biology 60:180–206
    [Google Scholar]
  2. Beckwith J.R., Zipser D. 1970 The Lactose Operon New York: Cold Spring Harbor Press;
    [Google Scholar]
  3. Brenner M., Tisdale D., Loomis W.F. 1975; Techniques for rapid biochemical screening of large numbers of cell clones. Experimental Cell Research 90:249–252
    [Google Scholar]
  4. Brenner M., Dimond R.L., Loomis W.F. 1976; Rapid biochemical screening of large numbers of animal cell clones. Methods in Cell Biology 14:187–193
    [Google Scholar]
  5. Dimond R.L., Loomis W.F. 1976; Structure and function of β-glucosidases in Dictyostelium discoideum. . Journal of Biological Chemistry 251:2680–2687
    [Google Scholar]
  6. Dimond R.L., Brenner M., Loomis W.F. 1973; Mutations affecting N-acetylglucosamini- dase in Dictyostelium discoideum. . Proceedings of the National Academy of Sciences of the United States of America 703356–3360
    [Google Scholar]
  7. Free S.J., Loomis W.F. 1974; Isolation of mutations in Dictyostelium discoideum affecting α-mannosidase. Biochemie 56:1525–1528
    [Google Scholar]
  8. Free S.J., Cockburn A., Loomis W.F. 1976a; α-Mannosidase-2: a developmentally regulated enzyme in Dictyostelium discoideum. . Developmental Biology 49:539–543
    [Google Scholar]
  9. Free S.J., Schimke R.T., Loomis W.F. 1976b; Genetic analysis of the gene for a- mannosidase in Dictyostelium discoideum. . Genetics 84:159–174
    [Google Scholar]
  10. Lewin B. 1975 Gene Expression I pp. 288–290 New York: John Wiley;
    [Google Scholar]
  11. Loomis W.F. 1970; Developmental regulation of α-mannosidase in Dictyostelium discoideum. . Journal of Bacteriology 103:375–381
    [Google Scholar]
  12. Loomis W.F. 1971; Sensitivity of Dictyostelium discoideum to nucleic acid analogues. Experimental Cell Research 64:484–486
    [Google Scholar]
  13. Loomis W.F. 1975 Dictyostelium discoideum: A Developmental System New York: Academic Press;
    [Google Scholar]
  14. Loomis W.F. 1978a; Genetic analysis of the gene for N-acetylglucosaminidase in Dictyostelium discoideum. . Genetics 88:277–284
    [Google Scholar]
  15. Loomis W.F. 1978b; The number of developmental genes in Dictyostelium. . Birth Defects 14:497–505
    [Google Scholar]
  16. Loomis W.F. 1980; A β-glucosidase gene in Dictyostelium discoideum. . Developmental Genetics in the Press
    [Google Scholar]
  17. Loomis W.F., Dimond R.L., Free S.J., White S.S. 1977; Independent and dependent sequences in development of Dictyostelium. . In Eucaryotic Microbes as Model Developmental Systems pp. 177–194 Edited by O’Day D., Horgan P. New York: Marcel Dekker;
    [Google Scholar]
  18. Ma G., Firtel R.A. 1978; Regulation of the synthesis of two carbohydrate binding proteins in Dictyostelium discoideum. . Journal of Biological Chemistry 253:3924–3932
    [Google Scholar]
  19. Macleod C.L. 1979 Gene expression in Dictyostelium discoideum: a genetic and biochemical analysis Ph.D. thesis University of California, San Diego, California; U.S.A.:
    [Google Scholar]
  20. Macleod C., Firtel R., Papkoff J. 1980; Regulation of actin gene expression during spore germination in Dictyostelium discoideum. . Developmental Biology 76:263–274
    [Google Scholar]
  21. Metzenberg R. 1979; Implication of some genetic control mechanisms in Neurospora. . Microbiological Reviews 43:361–383
    [Google Scholar]
  22. Sadler J.R., Smith T.F. 1971; Mapping of the lactose operator. Journal of Molecular Biology 62:139–170
    [Google Scholar]
  23. Sussman M. 1965; Temporal, spatial, and quanti-tative control of enzyme activity during slime mold cytodifferentiation. Brookhaven Symposia in Biology 18:66–76
    [Google Scholar]
  24. Sussman M. 1966; Biochemical and genetic methods in the study of cellular slime mold development. Methods in Cell Physiology 2:397–410
    [Google Scholar]
  25. Watts D.J., Ashworth J.M. 1970; Growth of myxamoebae of the cellular slime mold D. discoideum in axenic culture. Biochemical Journal 119:171–174
    [Google Scholar]
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