1887

Abstract

fusion strains of K12 have been constructed using the Casadaban (1976) gene-fusion technique. The major modification to the procedure was the removal of the guanine requirement of Mu-induced auxotrophs by introduction of a ColEl- plasmid prior to selection of the fusion. Conversion to prototrophy was necessary to overcome problems associated with repression of the operon by guanine added to selection media. Induction of the lysogenic fusion strains yielded plaque-forming transducing phages that carried the genes and either the complete gene and the promoter or only a distal portion of .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-126-2-497
1981-10-01
2024-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/126/2/mic-126-2-497.html?itemId=/content/journal/micro/10.1099/00221287-126-2-497&mimeType=html&fmt=ahah

References

  1. Barker D. F., Campbell A. M. 1980; Use of bio-lac fusion strains to study regulation of biotin biosynthesis in Escherichia coli. Journal of Bacteriology 143:789–800
    [Google Scholar]
  2. Casadaban M. J. 1976; Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. Journal of Molecular Biology 104:541–555
    [Google Scholar]
  3. Clarke L., Carbon J. 1979; Selection of specific clones from colony banks by suppression or complementation tests. Methods in Enzymology 68:396–408
    [Google Scholar]
  4. Gilbert H. J., Drabble W. T. 1980; Complementation in vitro between guaB mutants of Escherichia coli K12. Journal of General Microbiology 117:33–45
    [Google Scholar]
  5. Gilbert H. J., Lowe C. R., Drabble W. T. 1979; Inosine 5′-monophosphate dehydrogenase of Escherichia coli: purification by affinity chromatography, subunit structure and inhibition by guanosine 5′-monophosphate. Biochemical Journal 183:481–494
    [Google Scholar]
  6. Howe M. M. 1973; Prophage deletion mapping of bacteriophage Mu-1. Virology 54:93–101
    [Google Scholar]
  7. Lambden P. R. 1972 Gene-enzyme relationships in the purine pathway of Escherichia coli. Ph.D. thesis. University of Southampton, U.K.:
    [Google Scholar]
  8. Lambden P. R., Drabble W. T. 1973; The gua operon of Escherichia coli K12: evidence for polarity from guaB to guaA. Journal of Bacteriology 115:992–1002
    [Google Scholar]
  9. Leathers T. D., Noti J., Umbarger H. E. 1979; Physical characterisation of ilv-lac fusions. Journal of Bacteriology 140:251–260
    [Google Scholar]
  10. Mehra R. K., Drabble W. T. 1981; Dual control of the gua operon of Escherichia coli K12 by adenine and guanine nucleotides. Journal of General Microbiology 123:27–37
    [Google Scholar]
  11. Nicolaidis A. A., Drabble W. T. 1979; Plasmid replication in Salmonella typhimurium LT2 and Escherichia coli K-12: a differential effect of nalidixic acid. FEMS Microbiology Letters 6:261–264
    [Google Scholar]
  12. Nijkamp H. J. J., DeHaan P. G. 1967; Genetic and biochemical studies of the guanosine 5′- monophosphate pathway in Escherichia coli. Bio-chimica et biophysica acta 145:31–40
    [Google Scholar]
  13. Rosner J. L. 1972; Formation, induction and curing of bacteriophage P1 lysogens. Virology 48:679–689
    [Google Scholar]
  14. Schrenk W. J., Weisberg R. A. 1975; A simple method for making new transducing lines of coliphage λ. Molecular and General Genetics 137:101–107
    [Google Scholar]
  15. Shimada K., Weisberg R., Gottesman M. 1972; Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. Journal of Molecular Biology 63:483–503
    [Google Scholar]
  16. Shimada K., Fukumaki U., Takagi Y. 1976; Expression of the guanine operon of Escherichia coli as analysed by bacteriophage lambda-induced mutations. Molecular and General Genetics 147:203–208
    [Google Scholar]
  17. Smith J. M., Gots J. S. 1980; purF-lac fusion and direction of purF transcription in Escherichia coli. Journal of Bacteriology 143:1156–1164
    [Google Scholar]
  18. Vales L. D., Chase J. W., Murphy J. B. 1979; Orientation of the guanine operon of Escherichia coli K-12 by utilising strains containing guaB-xse and guaB-upp deletions. Journal of Bacteriology 139:320–322
    [Google Scholar]
  19. Vogel H. J., Bonner D. M. 1956; Acetyl-ornithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  20. Zeldis J. B., Bukhari A. I., Zipser D. 1973; Orientation of prophage Mu. Virology 55:289–294
    [Google Scholar]
  21. Zubay G. 1973; In vitro synthesis of protein in microbial systems. Annual Review of Genetics 7:267–287
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-126-2-497
Loading
/content/journal/micro/10.1099/00221287-126-2-497
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error