RT Journal Article SR Electronic(1) A1 Sahl, H.-G. A1 Brandis, H.YR 1981 T1 Production, Purification and Chemical Properties of an Antistaphylococcal Agent Produced by Staphylococcus epidermidis JF Microbiology, VO 127 IS 2 SP 377 OP 384 DO https://doi.org/10.1099/00221287-127-2-377 PB Microbiology Society, SN 1465-2080, AB An antibacterial agent produced by Staphylococcus epidermidis strain 5 was isolated from culture supernatants; its spectrum of activity is restricted to staphylococci and micrococci. The bacteriocin-like substance was purified to homogeneity by column chromatography on Servachrome XAD-2, CM-Sephadex C-25 and Sephadex G-50. Chemical analysis showed it to be a peptide with a molecular weight of about 6000 and an isoelectric point of about 10ยท5. Purified material was rapidly inactivated by trypsin, chymotrypsin and pronase and it was relatively heat-stable. After acidic hydrolysis 10 amino acid residues and two unidentified ninhydrin-positive substances were detected. Production of the bacteriocin-like substance was not inducible with mitomycin C., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-127-2-377