@article{mbs:/content/journal/micro/10.1099/00221287-129-2-321, author = "Mergeay, Max and Gerits, Jozef", title = "Transduction of Escherichia coli trp Genes in Salmonella typhimurium and Effect of N-Methyl-N′-Nitro-N-Nitrosoguanidine on Transduction with Heterogenotic DNA", journal= "Microbiology", year = "1983", volume = "129", number = "2", pages = "321-335", doi = "https://doi.org/10.1099/00221287-129-2-321", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-129-2-321", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: P1Kc-mediated transduction of Escherichia coli trp genes occurred at a frequency of about 10−8 in Salmonella typhimurium trp strains carrying mutations determining sensitivity to P1 and a low level of restriction enzymes. Heterospecific transductants were analysed by using them as donors in second-stage transductions mediated by bacteriophage KB int. One class of heterospecific transductants had the phenotype Trp+ Pro− but were extremely unstable and reverted at high frequency (up to 80%) to the parental phenotype. The Trp+ Pro− phenotype probably represents insertions of the E. coli trp genes in the S. typhimurium pro genes. It was stable in a RecA background.", }