1887

Abstract

Extracts of Bacillus sphaericus NCTC 9602 catalysed the formation of -diaminopimelate from aspartic -semialdehyde plus pyruvate, or from dihydrodipicolinate, even though no activities of tetrahydrodipicolinate acetylase (or succinylase) nor -acetyl-(or -succinyl-)-diaminopimelate deacylase nor diaminopimelate epimerase were found. However, -diaminopimelate -dehydrogenase was present, and had very high activity at pH 7.5 in the direction of synthesis of -diaminopimelate from tetrahydrodipicolinate. A lysine-requiring mutant of lacked diaminopimelate dehydrogenase, and this enzyme reappeared in a revertant that grew without lysine. Other lysine-requiring auxotrophs were defective in dihydrodipicolinate synthase or dihydrodipicolinate reductase or diaminopimelate decarboxylase, but had diaminopimelate dehydrogenase. Diaminopimelate dehydrogenase is not important in the assimilation of ammonia. Mutants that lack this enzyme or else cannot make one of its substrates (tetrahydrodipicolinate) still grow rapidly in minimal medium (plus 0.7 m--lysine) containing ammonium chloride (36 m) as the only major source of nitrogen. The wild-type grew with -glutamine, but not with glutamate or lysine as sole source of nitrogen.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-3-739
1983-03-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/3/mic-129-3-739.html?itemId=/content/journal/micro/10.1099/00221287-129-3-739&mimeType=html&fmt=ahah

References

  1. Antia M., Work E. 1961; Oxidation ofmeso-a,s-diaminopimelic acid by certain sporulating species of bacteria. Journal of General Microbiology 26:67–80
    [Google Scholar]
  2. Antia M., Hoare D. S., Work E. 1957; The stereoisomers of a s-diaminopimelic acid. 3. Properties and distribution of diaminopimelic acid race- mase, an enzyme causing interconversion of the LL andmeso isomers. Biochemical Journal 65:448–459
    [Google Scholar]
  3. Black S., Wright N. G. 1955a; Aspartic β- semialdehyde dehydrogenase and aspartic β-semialdehyde. Journal of Biological Chemistry 213:39–50
    [Google Scholar]
  4. Black S., Wright N. G. 1955b; Homoserine dehydrogenase. Journal of Biological Chemistry 213:51–60
    [Google Scholar]
  5. Chatterjee S. P., White P. J. 1982; Activities and regulation of the enzymes of lysine biosynthesis in a lysine-excreting strain ofBacillus megaterium. Journal of General Microbiology 128:1073–1081
    [Google Scholar]
  6. Farkas W., Gilvarg C. 1965; The reduction step in diaminopimelic acid synthesis. Journal of Biological Chemistry 240:4717–4722
    [Google Scholar]
  7. Gilvarg C. 1958; The enzymatic synthesis of diaminopimelic acid. Journal of Biological Chemistry 233:1501–1504
    [Google Scholar]
  8. Gilvarg C. 1959; N-succinyl-l-diaminopimelic acid. Journal of Biological Chemistry 234:2955–2959
    [Google Scholar]
  9. Gilvarg C. 1962; Diaminopimelic acid synthesis inEscherichia coli. Methods in Enzymology 5:848–850
    [Google Scholar]
  10. Hungerer K. D., Tipper D. J. 1969; Cell wall polymers ofBacillus sphaericus 9602. 1. Structure of the vegetative cell wall peptidoglycan. Biochemistry 8:3577–3587
    [Google Scholar]
  11. Imae Y., Strominger J. L. 1976; Relationship between cortex content and properties ofBacillus sphaericus spores. Journal of Bacteriology 126:907–913
    [Google Scholar]
  12. Kimura K. 1974; Pyridine-2,6-dicarboxylic acid (dipicolinic acid) formation inBacillus subtilis. 1. Non-enzymatic formation of dipicolinic acid from pyruvate and aspartic semialdehyde. Journal of Biochemistry 75:961–967
    [Google Scholar]
  13. Kimura K. 1975; A new flavin enzyme catalysing the reduction of dihydrodipicolinate in sporulatingBacillus subtilis. 1. Purification and properties. Journal of Biochemistry 77:405–413
    [Google Scholar]
  14. Kindler S. H. 1962; N-Succinyl-l-diaminopimelic deacylase. Methods in Enzymology 5:851–853
    [Google Scholar]
  15. Linnett P. E., Tipper D. J. 1976; Transcriptional control of peptidoglycan precursor synthesis during sporulation inBacillus sphaericus. Journal of Bacteriology 125:565–574
    [Google Scholar]
  16. Meadow P., Work E. 1958a; The effects of vitamin B6 and its derivatives on diaminopimelic acid decarboxylase inBacillus sphaericus asporogenous. Biochimica et biophysica acta 29:180–187
    [Google Scholar]
  17. Meadow P., Work E. 1958b; Bacterial transamination of the stereoisomers of diaminopimelic acid and lysine. Biochimica et biophysica acta 28:596–599
    [Google Scholar]
  18. Meers J. L., Tempest D. W., Brown C. M. 1970; ‘Glutamine (amide): 2-oxoglutarate amino transferase oxido-reductase (NADP)’;, an enzyme involved in the synthesis of glutamate by some bacteria. Journal of General Microbiology 64:187–194
    [Google Scholar]
  19. Meister A. 1965 Biochemistry of the Amino Acids 2, 2nd edn. pp. 928–951 New York: Academic Press;
    [Google Scholar]
  20. Milner H. W., Lawrence N. S., French C. S. 1950; Colloidal dispersion of chloroplast material. Science 111:633–634
    [Google Scholar]
  21. Misono H., Soda K. 1980; Properties ofmeso-a,s-diaminopimelate D-dehydrogenase fromBacillus sphaericus. Journal of Biological Chemistry 255:10599–10605
    [Google Scholar]
  22. Misono H., Togawa EL, Soda K. 1979; mes0-α,ε-Diaminopimelate d-dehydrogen- ase: distribution and the reaction product. Journal of Bacteriology 137:22–27
    [Google Scholar]
  23. Powell J. F., Strange R. E. 1957; α ε-Diaminopimelic acid metabolism and sporulation inBacillus sphaericus. Biochemical Journal 65:700–708
    [Google Scholar]
  24. Rhuland L. E., Work E., Denman R. F., Hoare D. S. 1955; The behaviour of isomers ofa,e-diaminopimelic acid on paper chromatograms. Journal of the American Chemical Society 77:4844–4846
    [Google Scholar]
  25. Saleh F., White P. J. 1976; Use of auxotrophic mutants to isolate ll- or dd-isomers of 2,6-diamino- pimelic acid. Journal of General Microbiology 96:253–261
    [Google Scholar]
  26. Shedlarsky J. G. 1971; Pyruvate-aspartic semialdehyde condensing enzyme (Escherichia coli). Methods in Enzymology 17B:129–134
    [Google Scholar]
  27. Sundharadas G., Gilvarg C. 1967; Biosynthesis of α,ε-diaminopimelic acid inBacillus megaterium. Journal of Biological Chemistry 242:3983–3984
    [Google Scholar]
  28. Tamir H. 1971; Dihydrodipicolinic acid reductase(Escherichia coli). Methods in Enzymology 17B:134–139
    [Google Scholar]
  29. Vogel H. J., Thompson J. S., Shockman G. D. 1970; Characteristic metabolic patterns of prokaryotes and eukaryotes. Symposia of the Society for General Microbiology 20:107–119
    [Google Scholar]
  30. Weinberger S., Gilvarg C. 1970; Bacterial distribution of the use of succinyl and acetyl blocking groups in diaminopimelic acid biosynthesis. Journal of Bacteriology 101:323–324
    [Google Scholar]
  31. White P. J. 1971; Diaminopimelate decarboxylase(Escherichia coli). Methods in Enzymology 17B:140–145
    [Google Scholar]
  32. White P. J. 1972; The nutrition ofBacillus megaterium andBacillus cereus. Journal of General Microbiology 71:505–515
    [Google Scholar]
  33. White P. J. 1979; Effects of d-glutamate on enzymes of ammonia assimilation inBacillus megateriumNCIB 7581. Journal of General Microbiology 114:159–168
    [Google Scholar]
  34. White P. J., Lotay H. K. 1980; Minimal nutritional requirements ofBacillus sphaericusNCTC 9602 and 26 other strains of this species: the majority grow and sporulate with acetate as sole major source of carbon. Journal of General Microbiology 118:13–19
    [Google Scholar]
  35. White P. J., Lejeune B., Work E. 1969; Assay and properties of diaminopimelate epimerase fromBacillus megaterium. Biochemical Journal 113:589–601
    [Google Scholar]
  36. Work E. 1957; Reaction of ninhydrin in acid solution with straight chain amino acids containing two amino groups and its application to the estimation of α, ε-diaminopimelic acid. Biochemical Journal 67:416–423
    [Google Scholar]
  37. Yamakura F., Ikeda Y., Kimura K., Sasakawa T. 1974; Partial purification and some properties of pyruvate-aspartic semialdehyde condensing enzyme from sporulatingBacillus subtilis. Journal of Biochemistry 76:611–621
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-3-739
Loading
/content/journal/micro/10.1099/00221287-129-3-739
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error