1887

Abstract

Prime plasmids have been isolated from AS1 using the IncP-1 plasmid pMO172. Such plasmids can complement mutant function when transferred to appropriate strains of PAO. From the range of particular functions complemented by each prime plasmid, a preliminary map of the AS1 genome with four groups of linked markers was obtained. Physical examination of two of these prime plasmids showed that each had acquired an additional DNA segment. Southern hybridization experiments showed there was sequence homology between the additional DNA segments and AS1 DNA, confirming that these prime plasmids carried a segment of the AS1 genome. Mapping by complementation may be applicable to other bacteria in which mutants suitable for selecting recombinants are not readily available.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-3-785
1983-03-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/3/mic-129-3-785.html?itemId=/content/journal/micro/10.1099/00221287-129-3-785&mimeType=html&fmt=ahah

References

  1. Calhoun D.H., Pierson D.L., Jensen R.A. 1973; The regulation of tryptophan biosynthesis in Pseudomonas aeruginosa.. Molecular and General Genetics 121:117–132
    [Google Scholar]
  2. Haas D., Holloway B.W. 1976; R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa.. Molecular and General Genetics 144:243–251
    [Google Scholar]
  3. Holloway B.W. 1965; Variations in restriction and modification following increase of growth temperature of Pseudomonas aeruginosa.. Virology 25:634–642
    [Google Scholar]
  4. Holloway B.W. 1978; Isolation and characterisation of an R´ plasmid in Pseudomonas aeruginosa.. Journal of Bacteriology 133:1078–1082
    [Google Scholar]
  5. Holloway B.W. 1979; Plasmids that mobilize bacterial chromosome.. Plasmid 2:1–19
    [Google Scholar]
  6. Holloway B.W. 1981; The application of pseudomonad-based genetics to methylotrophs.. In Microbial Growth on C1,Compounds. Proceedings of the Third International Symposium, Sheffield pp. 317–324 Edited by Dalton H. London: Heyden & Son;
    [Google Scholar]
  7. Holloway B.W., Crockett R.J. 1982 Pseudomonas aeruginosa. Genetic Maps 2:165–167
    [Google Scholar]
  8. Holloway B.W., Van De Putte P. 1968; Lysogeny and bacterial recombination.. In Replication and Recombination of Genetic Material pp. 175–183 Edited by Peacock W. J., Brock R. D. Canberra: Australian Academy of Science;
    [Google Scholar]
  9. Holloway B.W., Krishnapillai V., Morgan A.F. 1979; Chromosomal genetics of Pseudomonas.. Microbiological Reviews 43:73–102
    [Google Scholar]
  10. Holloway B.W., Crowther C., Royle P., Nayudu M. 1980; R-plasmids and bacterial chromosome transfer.. In Antibiotic Resistance, Transposition and Other Mechanisms. Fourth Symposium on Antibiotic Resistance, Smolenice pp. 19–27 Edited by Mitsuhashi S., Rosival L., Krcmery V. Prague: Avicenum;
    [Google Scholar]
  11. Isaac J.H., Holloway B.W. 1968; Control of pyrimidine biosynthesis in Pseudomonas aeruginosa.. Journal of Bacteriology 96:1732–1741
    [Google Scholar]
  12. Isaac J.H., Holloway B.W. 1972; Control of arginine biosynthesis in Pseudomonas aeruginosa.. Journal of General Microbiology 73:427–438
    [Google Scholar]
  13. Jacob A.E., Creswell J.M., Hedges R.W. 1977; Molecular characterization of the P group plasmid R68 and variants with enhanced chromosomal mobilizing ability.. FEMS Microbiology Letters 1:71–74
    [Google Scholar]
  14. Johnston A.W.B., Setchell S.M., Beringer J.E. 1978; Interspecific crosses between Rhizobium leguminosarum and R. meliloti: formation of haploid recombinants and of R-primes.. Journal of General Microbiology 104:209–218
    [Google Scholar]
  15. Krishnapillai V. 1971; A novel transducing phage. Its role in recognition of a possible new hostcontrolled modification system in Pseudomonas aeruginosa.. Molecular and General Genetics 114:134–143
    [Google Scholar]
  16. Leemans J., Villarroel R., Silva B., Van Montagu M., Schell J. 1980; Direct repetition of a 1-2 md DNA sequence is involved in site specific recombination by the PI plasmid R68.. Gene 10:319–328
    [Google Scholar]
  17. Levinthal M. 1974; Bacterial genetics excluding E. coli.. Annual Review of Genetics 8:219–230
    [Google Scholar]
  18. Low K.B. 1972; Escherichia coli K12 F-prime factors, old and new.. Bacteriological Reviews 36:587–607
    [Google Scholar]
  19. Morgan A.F. 1979; Transduction of Pseudomonas aeruginosawith a mutant of bacteriophage E79.. Journal of Bacteriology 139:137–140
    [Google Scholar]
  20. Morgan A.F. 1982; Isolation and characterization of Pseudomonas aeruginosa R´ plasmids constructed by means of interspecific mating.. Journal of Bacteriology 149:654–661
    [Google Scholar]
  21. Nagahari K., Sano Y., Sakaguchi K. 1977; Derepression of E. coli trp operon on interfamilial transfer.. Nature, London 266:745–746
    [Google Scholar]
  22. Nayudu M., Holloway B.W. 1981; Isolation and characterisation of R plasmid variants with enhanced chromosomal mobilisation ability in E. coli K12.. Plasmid 6:53–65
    [Google Scholar]
  23. O’Connor M.L., Hanson R.S. 1978; Linkage relationships between mutants of Methylobacterium organophilumimpaired in their ability to grow on one-carbon compounds.. Journal of General Microbiology 104:105–111
    [Google Scholar]
  24. Pemberton J.M., Holloway B.W. 1972; Chromosome mapping in Pseudomonas aeruginosa.. Genetical Research 19:251–260
    [Google Scholar]
  25. Pemberton J.M., Holloway B.W. 1973; A new sex factor of Pseudomonas aeruginosa.. Genetical Research 21:263–272
    [Google Scholar]
  26. Riess G., Holloway B.W., Pühler A. 1980; R68.45, a plasmid with chromosome mobilizing ability (Cma), carries a tandem duplication.. Genetical Research 36:99–109
    [Google Scholar]
  27. Royle P.L., Holloway B.W. 1980; Relationship between R and FP plasmids in Pseudomonas aeruginosa.. Antimicrobial Agents and Chemotherapy 17:293–297
    [Google Scholar]
  28. Southern E. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis.. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  29. Willetts N.S., Crowther C., Holloway B.W. 1981; The insertion sequence IS21 of R68.45 and the molecular basis for mobilization of the bacterial chromosome.. Plasmid 6:30–52
    [Google Scholar]
  30. Wlndass J.D., Worsey M.J., Ploli E.M., Ploli D., Barth P.T., Atherton K.T., Dart E.C., Byrom D., Powell K., Senior P.K. 1980; Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus.. Nature, London 287:396–401
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-3-785
Loading
/content/journal/micro/10.1099/00221287-129-3-785
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error