1887

Abstract

strains NZP2037 and NZP2213 were each found to contain a single large plasmid: pRlo2037 (240 MDal) and pRlo2213 (120 MDal), respectively. Plasmid DNA present in crude cell lysates of each strain and purified pRlo2037 DNA did not hybridize with pIDl, a recombinant plasmid containing part of the nitrogen fixation () region of , indicating that genes were not present on these plasmids. The transposon Tn5 was inserted into pRlo2037 and this plasmid was then transferred into and All transconjugants failed to nodulate , suggesting that the ability to nodulate this legume was also not carried on pRlo2037. Transfer of pRlo2037 to strain NZP2213 did not alter the Nod Fix phenotype of this strain for Determinants for flavolan resistance, believed to be necessary for effective nodulation of , were not carried on pRlo2037. These data suggest that nodulation, nitrogen fixation and flavolan resistance genes are not present on the large plasmid in strain NZP2037.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-8-2535
1983-08-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/8/mic-129-8-2535.html?itemId=/content/journal/micro/10.1099/00221287-129-8-2535&mimeType=html&fmt=ahah

References

  1. Banfalvi Z., Sakanyan V., Koncz C., Kiss A., Dusha I., Kondorosi A. 1981; Location of nodulation and nitrogen fixation genes on a high molecular weight plasmid of R. meliloti. Molecular and General Genetics 184:318–325
    [Google Scholar]
  2. Beringer J.E. 1974; R factor transfer in Rhizobium leguminosarum. . Journal of General Microbiology 84:188–198
    [Google Scholar]
  3. Beringer J.E., Beynon J.L., Buchanan-Wollaston A. V., Johnston A.W.B. 1978; Transfer of the drug-resistance transposon Tn5 to Rhizobium. Nature; London: 276633–634
    [Google Scholar]
  4. Brewin N.J., Dejong T.M., Phillips D.A., Johnston A.W.B. 1980; Cotransfer of determinants of hydrogenase activity and nodulation ability in Rhizobium leguminosarum. Nature; London: 28877–79
    [Google Scholar]
  5. Broughton W.J., Van Egeraat A.W.S.M., Lie T.A. 1980; Dynamics of Rhizobium competition for nodulation of Pisum sativum cv. Afghanistan. Canadian Journal of Microbiology 26:562–565
    [Google Scholar]
  6. Cannon F.C., Riedel G.E., Ausubel F.M. 1979; Overlapping sequences of Klebsiella pneumoniae ni/DNA cloned and characterized. Molecular and General Genetics 174:59–66
    [Google Scholar]
  7. Cantrell M.A., Hickok R.E., Evans H.J. 1982; Identification and characterization of plasmids in hydrogen uptake positive and hydrogen uptake negative strains of Rhizobium japonicum. . Archives of Microbiology 131:102–106
    [Google Scholar]
  8. Casse F., Boucher C., Julliot J.S., Michel M., Dénarié J. 1979; Identification and characterization of large plasmids in Rhizobium meliloti using agarose gel electrophoresis. Journal of General Microbiology 114:257–266
    [Google Scholar]
  9. Denhardt D.T. 1966; A membrane-filter technique for the detection of complementary DNA. Biophysical and Biochemical Research Communications 23:641–646
    [Google Scholar]
  10. Eckhardt T. 1978; A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. Plasmid 1:584–588
    [Google Scholar]
  11. Gross D.C., Vidaver A.K., Klucas R.V. 1979; Plasmids, biological properties and efficacy of nitrogen fixation in Rhizobium japonicum strains indigenous to alkaline soils. Journal of General Microbiology 114:257–266
    [Google Scholar]
  12. Haugland R., Verma D.P.S. 1981; Interspecific plasmid and genomic DNA sequence homologies and localization of nif genes in effective and ineffective strains of Rhizobium japonicum. . Journal of Molecular and Applied Genetics 1:205–217
    [Google Scholar]
  13. Helling R.B., Goodman H.M., Boyer H.W. 1974; Analysis of endonuclease R.Eco-RI fragments of DNA from lambdoid bacteriophages and other viruses by agarose gel electrophoresis. Journal of Virology 14:1235–1244
    [Google Scholar]
  14. Hirsch P.R., Van Montagu M., Johnston A.W.B., Brewin N.J., Schell J. 1980; Physical identification of bacteriocinogenic, nodulation and other plasmids in strains of Rhizobium leguminosarum. . Journal of General Microbiology 120:403–412
    [Google Scholar]
  15. Hombrecher G., Brewin N.J., Johnston A.W.B. 1981; Linkage of genes for nitrogenase and nodulation ability on plasmids in Rhizobium leguminosarum and R. phaseoli. Molecular and General Genetics 182:133–136
    [Google Scholar]
  16. Hooykaas P.J.J., Van Brussel A.A.N., Dendulk-Ras H., Van Slogteren G.M.S., Schilperoort R.A. 1981; Symplasmid of Rhizobium trifolii expressed in different rhizobial species and in Agrobacterium tumefaciens. . Nature; London: 291351–353
    [Google Scholar]
  17. Jarvis B.W.D., Pankhurst C.E., Patel J.J. 1982; Rhizobium loti, a new species of legume root nodule bacteria. International Journal of Systematic Bacteriology 32:378–380
    [Google Scholar]
  18. Johnston A.W.B., Beynon J.L., Buchanan-Wollaston A.V., Setchell S.M., Hirsch P.R., Beringer J.E. 1978; High frequency transfer of nodulating ability between strains and species of Rhizobium. Nature; London: 276634–636
    [Google Scholar]
  19. Kondorosi A., Kondorosi E., Pankhurst C.F., Broughton W.J., Banfalvi Z. 1982; Mobilization of a Rhizobium meliloti megaplasmid carrying nodulation and nitrogen fixation genes into other rhizobia and Agrobacterium. . Molecular and General Genetics 188:433–439
    [Google Scholar]
  20. Long S.R., Buikema W.J., Ausubel F.M. 1982; Cloning of Rhizobium meliloti nodulation genes by direct complementation of Nod mutants. Nature; London: 298485–488
    [Google Scholar]
  21. Maniatis T., Jeffrey A., Kleid D.G. 1975; Nucleotide sequence of the rightward operator of phage λ. Proceedings of the National Academy of Sciences of the United States of America 72:1184–1188
    [Google Scholar]
  22. Masterson R.V., Russell P.R., Atherly A.G. 1982; Nitrogen fixation (nif) genes and large plasmids of Rhizobium japonicum. . Journal of Bacteriology 152:928–931
    [Google Scholar]
  23. Nuti M.P., Ledeboer A.M., Lepidi A.A., Schilperoort R.A. 1977; Large plasmids in different Rhizobium species. Journal of General Microbiology 100:241–248
    [Google Scholar]
  24. Nuti M.P., Lepidi A.A., Prakash R.K., Schilperoort R.A., Cannon F.C. 1979; Evidence for nitrogen fixation (nif) genes on indigenous Rhizobium plasmids. Nature; London: 282533–535
    [Google Scholar]
  25. Pankhurst C.E. 1977; Symbiotic effectiveness of antibiotic-resistant mutants of fast- and slow-growing strains of Rhizobium nodulating Lotus species. Canadian Journal of Microbiology 23:1026–1033
    [Google Scholar]
  26. Pankhurst C.E. 1981; Effect of plant nutrient supply on nodule effectiveness and Rhizobium strain competition for nodulation of Lotus pedunculatus. Plant and Soil 60:325–339
    [Google Scholar]
  27. Pankhurst C.E., Jones W.T. 1979; Effectiveness of Lotus root nodules. II. Relationship between root nodule effectiveness and ‘in vitro’ sensitivity of fast-growing Lotus rhizobia to flavolans. Journal of Experimental Botany 30:1095–1107
    [Google Scholar]
  28. Pankhurst C.E., Jones W.T., Craig A.S. 1982; Bactericidal effect of Lotus pedunculatus root flavo-lan on fast-growing Lotus rhizobia. Journal of General Microbiology 128:1567–1576
    [Google Scholar]
  29. Prakash R.K., Schilperoort R.A., Nuti M.P. 1981; Large plasmids of fast-growing rhizobia: homology studies and location of structural nitrogen fixation (nif) genes. Journal of Bacteriology 145:1129–1136
    [Google Scholar]
  30. Rosenberg C., Boistard P., Dénarié J., Casse-Delbart F. 1981; Genes controlling early and late functions in symbiosis are located on a megaplasmid in Rhizobium meliloti. . Molecular and General Genetics 184:326–333
    [Google Scholar]
  31. Rosenberg C., Casse-Delbart F., Dusha I., David M., Boucher C. 1982; Megaplasmids in the plant-associated bacteria Rhizobium meliloti and Pseudomonas solanacearum. . Journal of Bacteriology 150:402–406
    [Google Scholar]
  32. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–518
    [Google Scholar]
  33. Trinick M.J. 1980; Relationship amongst the fastgrowing rhizobia of Lablab purpureus, Leucaena leucocephala, Mimosa spp., Acacia franesiana and Sesbania grandiflora and their affinities with other rhizobial groups. Journal of Applied Bacteriology 49:39–53
    [Google Scholar]
  34. Van Larebeke N., Engler G., Holsters M., Van Dene Lsecker S., Zaenen I., Schilperoort R.A., Schell J. 1974; Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing activity. Nature; London: 252169–170
    [Google Scholar]
  35. Wahl G.M., Stern M., Stark G.R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridisation by using dextran sulfate. Proceedings of the National Academy of Sciences of the United States of America 76:3683–3687
    [Google Scholar]
  36. Zurkowski W., Lorkiewicz Z. 1979; Plasmid mediated control of nodulation in Rhizobium trifolii. Archives of Microbiology 123:195–201
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-8-2535
Loading
/content/journal/micro/10.1099/00221287-129-8-2535
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error