Adenine Phosphoribosyltransferase Mutants in Saccharomyces cerevisiae
Woods, Robin A. and Roberts, Darlene G. and Stein, David S. and Filpula, David,, 130, 2629-2637 (1984),
doi = https://doi.org/10.1099/00221287-130-10-2629,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Mutants of Saccharomyces cerevisiae deficient in adenine phosphoribosyltransferase (A-PRT, EC 2.4.2.7) have been isolated following selection for resistance to 8-azaadenine in a prototrophic strain carrying the ade4-su allele of the gene coding for amidophosphoribosyltransferase(EC 2.4.2.14). The mutants were recessive and defined a single gene, aptl. They did not excrete purine when combined with ade4+. The mutants appeared to retain some A-PRT activity in crude extracts, and strains of the genotype ade2 aptl responded to both adenine and hypoxanthine. Mutants deficient in adenine aminohydrolase (EC 3.5.4.2) activity, aahl, and hypoxanthine: guanine phosphoribosyltransferase (EC 2.4.2.8) activity, hptl, were used to synthesize the genotypes aptl hptl aah+ and aptl hpt+ aahl. The absence of A-PRT activity in strains with these genotypes confirmed the hypothesis that the residual A-PRT activity of aptl mutants was due to adenine aminohydrolase and hypoxanthine:guanine phosphoribosyltransferase acting in concert.,
language=,
type=