1887

Abstract

The surface charge against pH behaviour of spores of type A was determined using a colloid titration method in the pH range 4–9. The native spores as well as various ionic forms of the spores loaded with divalent cations were found to be negatively charged. A positive colloid, MGCh, inhibited the ionic germination of the spores and this inhibition was highly dependent on pH. However, another positive colloid, GCh, did not inhibit the germination. Chemical modification of carboxyl groups on the spore surface by use of a water-soluble carbodiimide with two nucleophiles, glycine ethyl ester and taurine, caused spores not only to have little or no colloidal charge but also to lose the ability to germinate. The modification of carboxyl groups in the spore coat protein also resulted in elimination of, or marked reduction in its negative charge. These results suggest that carboxyl groups in the spore coat protein are the major negatively charged species on the spore surface. The role of surface charge in the ionic germination of the spores is discussed.

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/content/journal/micro/10.1099/00221287-130-2-267
1984-02-01
2024-04-20
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References

  1. Alderton G., Snell N. 1963; Base exchange and heat resistance in bacterial spores. Biochemical and Biophysical Research Communications 10:139–143
    [Google Scholar]
  2. Ando Y. 1975; Effect of lysozyme on ionic forms of spores of Clostridium perfringens type A. Journal of Bacteriology 122:794–795
    [Google Scholar]
  3. Ando Y. 1979; Spore lytic enzyme released from Clostridium perfringensspores during germination. Journal of Bacteriology 140:59–64
    [Google Scholar]
  4. Aronson A. I., Fitz-James P. C. 1976; Structure and morphogenesis of the bacterial spore coat. Bacteriological Reviews 40:360–402
    [Google Scholar]
  5. Douglas H. W. 1955; Electrophoretic studies on bacterial spores. Resting spores of B. megaterium and B. subtilis. Transactions of the Faraday Society 51:146–152
    [Google Scholar]
  6. Duncan C. L., Strong D. H. 1968; Improved medium for sporulation of Clostridium perfringens. Applied Microbiology 16:412–416
    [Google Scholar]
  7. Gore M. S., Sherekar S. V., Kumta U. S., Phondke G. P. 1978; Microelectrophoretic studies on spores of Bacillus licheniformis: influence of spore coat extraction procedures on electrophoretic mobility. Indian Journal of Biochemistry and Biophysics 15:299–303
    [Google Scholar]
  8. Gould G. W., Stubbs J. M., King W. L. 1970; Structure and composition of resistant layers in bacterial spore coats. Journal of General Microbiology 60:347–355
    [Google Scholar]
  9. Hoare D. G., Koshland D. E. JR 1967; A method for quantitative modification and estimation of carboxylic acid groups in proteins. Journal of Biological Chemistry 242:2447–2453
    [Google Scholar]
  10. Kondo M., Nishihara T. 1970; Studies on the bacterial spore coat. (2) Chemical structures of fractions isolated from the dormant spore coat of Bacillus megaterium. Japanese Journal of Bacteriology 25:215–221
    [Google Scholar]
  11. Labbe R. G., Reich R. R., Duncan C. L. 1978; Alteration in ultrastructure and germination of Clostridium perfringens type A spores following extraction of spore coats. Canadian Journal of Microbiology 24:1526–1536
    [Google Scholar]
  12. Levinson H. S., Hyatt M. T. 1969; Activation of Bacillus megaterium spore germination. In Spores IV pp. 262–275 Edited by Campbell L. L. Bethesda, Md., USA: American Society for Microbiology;
    [Google Scholar]
  13. Nishihara T., Yoshimoto I., Kondo M. 1981; Studies on the bacterial spore coat. IX. The role of surface charge in germination of Bacillus megaterium spores. Microbiology and Immunology 25:763–771
    [Google Scholar]
  14. Tochikubo K., Kojima K., Hachisuka Y. 1975; Electrokinetic charge of resting and germinated spores of Bacillus subtilis. In Spores VI pp. 526–530 Edited by Gerhardt P., Costilow R. N., Sadoff H. L. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  15. Tsuzuki T., Ando Y. 1982; Location of exchangeable cations within the spore of Clostridium perfringens. Japanese Journal of Bacteriology 37:875–881
    [Google Scholar]
  16. Watanabe K., Takesue S. 1976; Colloid titration for determining the surface charge of bacterial spores. Journal of General Microbiology 96:221–223
    [Google Scholar]
  17. Wyatt J., Waites W. M., Morris V. J. 1982; Microelectrophoretic studies of coat-defective spores of Bacillus megaterium. Journal of General Microbiology 128:1661–1666
    [Google Scholar]
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