1887

Abstract

Summary: A possible colonization factor, E8775, has previously been described for enterotoxigenic strains of serogroups O25, O115 and O167. Re-examination of these strains by immunodiffusion has revealed that the antigenic nature of this factor, renamed putative colonization factor (PCF) 8775, is more complex than was first thought. All the strains of serogroup O25 tested possessed two antigenic components, termed CS4 and CS6, and gave mannose-resistant haemagglutination (MRHA) of human and bovine erythrocytes. Spontaneous variants possessing CS6 only did not give MRHA. Strains of serogroups O115 and O167 had the antigenic components CS5 and CS6, and gave MRHA of human, bovine and guinea-pig erythrocytes. Using immune electron microscopy, the components CS4 and CS5 were identified as fimbriae. No fimbriae were associated with CS6.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-9-2319
1985-09-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/09/mic-131-9-2319.html?itemId=/content/journal/micro/10.1099/00221287-131-9-2319&mimeType=html&fmt=ahah

References

  1. Cravioto A., Scotland S.M., Rowe B. 1982; Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non enterotoxigenic strains of Escherichia coli isolated from humans. Infection and Immunity 36:189–197
    [Google Scholar]
  2. Duguid J. P., Gillies R. R. 1957; Fimbriae and adhesive properties in dysentery bacilli. Journal of Pathology and Bacteriology 74:397–411
    [Google Scholar]
  3. Evans D. G., Evans D. J. 1978; New surface associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups O6 and O8. Infection and Immunity 21:638–647
    [Google Scholar]
  4. Evans D. G., Silver R. P., Evans D. J., Chase D. G., Gorbach S. L. 1975; Plasmid-controlled colonization factor associated with virulence in Escherichia coli enterotoxigenic for humans. Infection and Immunity 12:656–667
    [Google Scholar]
  5. Evans D. G., Evans D. J., Tjoa W. 1977; Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infection and Immunity 18:330–337
    [Google Scholar]
  6. Isaacson R. E., Richter P. 1981; Escherichia coli 987P pilus: purification and partial characterisation. Journal of Bacteriology 146:784–789
    [Google Scholar]
  7. Knutton S., Lloyd D.R., Candy D.C.A., Mcneish A. S. 1984; Ultrastructural study of adhesion of enterotoxigenic Escherichia coli to erythrocytes and human intestinal epithelial cells. Infection and Immunity 44:519–527
    [Google Scholar]
  8. Levine M. M., Ristaino P., Marley C., Smyth C., Knutton S., Boedeker E., Black R., Young C., Clements M. L., Cheney C., Patnaik R. 1984; Coli surface antigens 1 and 3 of colonization factor antigen II-positive enterotoxigenic Escherichia coli: morphology, purification, and immune responses in humans. Infection and Immunity 44:409–420
    [Google Scholar]
  9. Morris J. A., Thorns C. J., Sojka W. J. 1980; Evidence for two adhesive antigens on the K99 reference strain Escherichia coli B41. Journal of General Microbiology 118107–113
    [Google Scholar]
  10. Mullany P., Field A. M., Mcconnell M. M., Scotland S. M., Smith H. R., Rowe B. 1983; Expression of plasmids coding for colonization factor antigen II (CFA/II) and enterotoxin produc tion in Escherichia coli. Journal of General Micro biology 129:3591–3601
    [Google Scholar]
  11. Ørskov F., Ørskov I. 1984; Serotyping of Escherichia coli. Methods in Microbiology 14:43–112
    [Google Scholar]
  12. Ørskov I., ØRSKOV F., Birch-Anderson A. 1980; Comparison of Escherichia coli fimbrial antigen F7 with type 1 fimbriae. Injection and Immunity 18:657–666
    [Google Scholar]
  13. Ouchterlony O. l949; Antigen-antibody reactions in gels. Acta pathologica et microbiologica scandinavia 26507–515
    [Google Scholar]
  14. Peñaranda M. E., Mann M. B., Evans D. G., Evans D. J. 1980; Transfer of an ST:LT:CFA/11 plasmid into Escherichia coli K-12 strain RR1 by cotransformation with pSC301 plasmid DNA. FEMS Microbiology Letters 8:251–254
    [Google Scholar]
  15. Scotland S. M., Richmond J.E., Rowe B. 1983; Adhesion of enteropathogenic strains of Escherichia coli (EPEC) to HEp-2 cells is not dependent on the presence of fimbria. FEMS Microbiology Letters 20:191–195
    [Google Scholar]
  16. Smith H. R., Cravioto A., Willshaw G. A., Mcconnell M. M., Scotland S. M., Gross R. J., Rowe B. 1979; A plasmid coding for the production of colonisation factor antigen I and heat stable enterotoxin in strains of Escherichia coli of serogroup O78. FEMS Microbiology Letters 6:255–260
    [Google Scholar]
  17. Smyth C. J. 1982; Two mannose-resistant haemag glutinins on enterotoxigenic Escherichia coli of serotype O6:Kl5:Hl6 or H isolated from travellers’ and infantile diarrhoea. Journal of General Micro biology 128:2081–2096
    [Google Scholar]
  18. Thomas L. V., Rowe B. 1982; The occurrence of colonization factors (CFA/1, CFA/11 and E8775) in enterotoxigenic Escherichia coli from various countries in South East Asia. Medical Microbiology and Immunology 171:85–90
    [Google Scholar]
  19. Thomas L. V., Cravioto A., Scotland S. M., Rowe B. 1982; New fimbrial antigenic type (E8775) that may represent a colonization factor in enterotoxigenic Escherichia coli in humans. Infection and Immunity 3S:1119–1124
    [Google Scholar]
  20. Väisänen V., Elo J., Tallgren L. G., Siitonen A., Mäkelä P. H., Svanborg-Edùn C., Källenius G., Svenson S. B., Hultberg H., Korhonen T. 1981; Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis. Lancet ii1366–1369
    [Google Scholar]
  21. Van Den Bosch J., Verboom Sohmer U., Postma P., De Graaf J., Mclaren D.M. 1980; Mannose sensitive and mannose-resistant adherence to human uroepithelial cells and urinary virulence of Escheri chia coli. Infection and Immunity 29:226–233
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-9-2319
Loading
/content/journal/micro/10.1099/00221287-131-9-2319
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error