1887

Abstract

Summary: The biochemical and chemotaxonomic properties of three previously undescribed strains from human dental root canal infections are presented. The strains were obligately anaerobic Gram-negative rods with fimbriae and a thick capsule-like structure. Carbohydrates were not fermented and agglutination tests were negative. The presence of -galactosidase, - and -glucosidase, --acetylglucosaminidase and -galactosidase was confirmed. The strains produced acetic and succinic acids as metabolic end products. They contained a peptidoglycan structure based upon -diaminopimelic acid (A1) and lacked respiratory quinones. The cellular fatty acids were mainly straight-chain saturated and methyl-branched molecules. High interstrain DNA homology was observed and the DNA base compositions were between 56 and 59 mol % G + C. These three strains appear to comprise the nucleus of a new genus of anaerobic, Gram-negative rods from odontogenic infections.

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1986-02-01
2024-03-28
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References

  1. Carlsson J. 1973; Simplified gas chromatographic procedure for identification of bacterial metabolic products. Applied Microbiology 25:287–289
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquin-ones in actinomycetes and related bacteria. Journal of General Microbiology 100:221–230
    [Google Scholar]
  3. Collins M. D., Shah H. N., Mitsuoka T. 1985; Reclassification of Bacteroides microfusus (Kaneuchi and Mitsuoka) in a new genus Rikenella, as Rikenella microfusus comb. nov. Systematic and Applied Microbiology 6:79–81
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. European Journal of Biochemistry 12:133–142
    [Google Scholar]
  5. Duerden B. T., Holbrook W. P., Collee T. G., Watt W. 1976; The characterisation of clinically important Gram negative anaerobic bacilli by conventional bacteriological tests. Journal of Applied Bacteriology 40:163–188
    [Google Scholar]
  6. Gillis M., De Ley H., Schleifer K. H. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. European Journal of Biochemistry 12:143–153
    [Google Scholar]
  7. Haapasalo M., Lounatmaa K., Ranta H., Shah H., Ranta K. 1985; Ultrastructure of Bacteroides capillus, B buccae, B. pentosaceus, B. oris, B. oralis. B. reroralis, and pentose sugar-fermenting Bacteroides sp. from humans with periapical osteitis: occurrence of external proteinaceous cell wall layer. International Journal of Systematic Bacteriology 35:65–72
    [Google Scholar]
  8. Hardy R. L., Holland D. O., Nayler J. H. C. 1955; One-phase solvent mixtures for the separation of amino acids. Analytical Chemistry 27:971–974
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobe Laboratory Manual. 4th edn. Blacksburg, Virginia: Virginia Polytechnic Institute and State University Anaerobe Laboratory.
    [Google Scholar]
  10. Huss V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Systematic and Applied Microbiology 4:184–192
    [Google Scholar]
  11. Knivett V. O., Shah H. N., McKee A. S., Hardie J. M. 1983; Numerical taxonomy of some non-saccharolytic and saccharolytic Bacteroides. Journal of Applied Bacteriology 55:71–80
    [Google Scholar]
  12. Korhonen T. K. 1979; Yeast cell agglutination by purified enterobacterial pili. FEMS Microbiology Letters 6:421–425
    [Google Scholar]
  13. Krieg N. R. (editor). 1984; Bergey’s Manual of Systematic Bacteriology, vol. 1. Ancaster, Ontario: Williams & Wilkins.
    [Google Scholar]
  14. Lounatmaa K., Mäkelä P. H., Sarvas M. 1976; Effect of polymyxin on the ultrastructure of the outer membrane of wild-type and polymyxin-resistant strains of Salmonella. Journal of Bacteriology 127:1400–1407
    [Google Scholar]
  15. Luft J. H. 1964; Electron microscopy of cell extraneous coats as revealed by ruthenium red staining. Journal of Cell Biology 23:54A
    [Google Scholar]
  16. Marmur J. 1961; A procedure for isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  17. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium, Nocardia and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. Journal of General Microbiology 88:200–204
    [Google Scholar]
  18. Möller A. J. 1966; Microbiological examination of root canals and periapical tissues of human teeth. PhD thesis, University of Gothenburg, Sweden.
    [Google Scholar]
  19. Okuda K., Slots J., Genco J. 1981; Bacteroides gingivalis, Bacteroides asaccharolyticus, and Bacteroides melaninogenicus subspecies: cell surface morphology and adherence to erythrocytes and human buccal epithelial cells. Current Microbiology 6:7–12
    [Google Scholar]
  20. Peterson P. K., Wilkinson B. J., Kim Y., Schmeling D., Quie P. G. 1978; Influence of encapsulation on staphylococcal opsonisation and phagocytosis by human polymorphonuclear leukocytes. Infection and Immunity 19:943–949
    [Google Scholar]
  21. Pruzzo C., Dainelli B., Ricchetti M. 1984; Piliated Bacteroides fragilis strains adhere to epithelial cells and are more sensitive to phagocytosis by human neutrophils than nonpiliated strains. Infection and Immunity 43:189–194
    [Google Scholar]
  22. Reeves H. C., Rabin R., Wegener W. S., Ajl S. J. 1971; Assay of enzymes of the tricarboxylic acid and glyoxylate cycles. Methods in Microbiology 6A:425–462
    [Google Scholar]
  23. Rhuland L. E., Work E., Denman R. F., Hoare D. S. 1955; The behavior of isomers of α,ε-diaminopimelic acid on paper chromatograms. Journal of the American Chemical Society 17:4844–4846
    [Google Scholar]
  24. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriological Reviews 36:407–477
    [Google Scholar]
  25. Shah H. N., Collins M. D. 1983; Genus Bacteroides: a chemotaxonomical perspective. Journal of Applied Bacteriology 55:403–416
    [Google Scholar]
  26. Shah H. N., Williams R. A. D. 1982; Dehydrogenase patterns in the taxonomy of Bacteroides. Journal of General Microbiology 128:2955–2965
    [Google Scholar]
  27. Shah H. N., Williams R. A. D., Bowden G. H., Hardie J. M. 1976; Comparison of the biochemical properties of Bacteroides melaninogenicus from human dental plaque and other sites. Journal of Applied Bacteriology 41:473–492
    [Google Scholar]
  28. Sundqvist G. 1976; Bacteriological studies on necrotic dental pulps. PhD thesis, University of Umeȧ, Sweden.
    [Google Scholar]
  29. Sundqvist G., Bloom G. D., Enberg K., Johansson E. 1982; Phagocytosis of Bacteroides melaninogenicus and Bacteroides gingivalis in vitro by human neutrophils. Journal of Periodontal Research 17:113–121
    [Google Scholar]
  30. Vera H. D., Power D. A. 1980; Culture media. In Manual of Clinical Microbiology, 3rd edn, pp. 965–999 Edited by E. H. Lennette, A. Balows, W. J. Hausler, Jr & J. P. Truant, Washington, DC: American Society for Microbiology.
    [Google Scholar]
  31. Wagner R. C. 1976; Effect of tannic acid on electron images of capillary endothelial cell membranes. JJournal of Ultrastructural Research 57:132–139
    [Google Scholar]
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