1887

Abstract

Summary: Some characteristics of the lytic development of the temperate phage ϕC31 in A3(2) were studied using a thermoinducible lysogen. The physiological state of the host and the culture medium influenced the production of progeny virus after induction. The latent period lasted 45 min and the rise period 20-30 min. RNA synthesis in induced cultures was reduced with respect to controls. This reduction was restricted to cellular transcription as evidenced by: (i) no stable RNA being synthesized in induced cultures, and (ii) the proportion of phage specific RNA increasing from 0·5% before induction to more than 30% in induced cultures. Host RNA synthesis proceeded throughout the lytic cycle. Protein synthesis was also reduced in induced cultures, although to a lesser extent than RNA synthesis. Phage DNA synthesis started at around 10 min postinduction, marking the division between the early and late periods of phage development. Host DNA synthesis occurred during the first 20 min after induction, and gradually decreased later.

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/content/journal/micro/10.1099/00221287-132-6-1695
1986-06-01
2024-04-26
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References

  1. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins.. Bacteriological Reviews 31:230–314
    [Google Scholar]
  2. Chater K. F. 1984; Morphological and physiological differentiation in Streptomyces.. in Microbial Development, pp 89–115 Editor Birkbeck Losick, Shapiro. L. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  3. Chater K. F., Wilde L. C. 1976; Restriction of a bacteriophage of Streptomyces albus G involving endonuclease Sal I.. Journal of Bacteriology 128:644–650
    [Google Scholar]
  4. Chater K. F., Bruton C. J., Suarez J. E. 1981; Restriction mapping of the DN A of the Streptomyces temperate phage φC31 and its derivatives.. Gene 14:183–194
    [Google Scholar]
  5. Chater K. F., Bruton C. J., King A. A., Suarez J. E. 1982a; The expression of Streptomyces and Escherichia coli drug-resistance determinants cloned into the Streptomyces phage φC3. Gene 19:21–32
    [Google Scholar]
  6. Chater K. F., Hopwood D. A., Kieser T., Thompson C. J. 1982b; Gene cloning in Streptomyces.. Current Topics in Microbiology and Immunology 96:69–95
    [Google Scholar]
  7. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. . Journal of Molecular Biology 12:829–842
    [Google Scholar]
  8. Hardisson C., Manzanal M. B., Salas J. A., Suarez J. E. 1978; Fine structure, physiology and biochemistry of arthrospore germination in Streptomyces antibioticus.. Journal of General Microbiology 105:203–214
    [Google Scholar]
  9. Hardisson C., Salas J. A., Guijarro J. A., Suarez J. E. 1980; Macromolecular synthesis during the germination of Streptomyces spores in a chemically defined medium.. FEMS Microbiology Letters 7:233–235
    [Google Scholar]
  10. Hirsch C. F., Ensign J. C. 1976; Heat activation of Streptomyces viridochromogenes spores.. Journal of Bacteriology 126:24–30
    [Google Scholar]
  11. Kolebrander P. E., Ernest Hemphill H., Whiteley H. R. 1972; Nucleic acid synthesis in bacteriophage SP02cl-infected Bacillus subtilis. . Journal of Virology 9:776–784
    [Google Scholar]
  12. Lomovskaya N. D., Mkrtumian N. M., Gostims- kaya L. 1970; Isolation and properties of an actinophage of Streptomyces coelicolor.. Genetika 6:135–137
    [Google Scholar]
  13. Lomovskaya N. D., Chater K. F., Mkrtumian N. M. 1980; Genetics and molecular biology of Streptomyces bacteriophages.. Microbiological Reviews 44:206–229
    [Google Scholar]
  14. Novikova N. L., Kapitonova O. N., Lomovskaya N. D. 1973; Thermal prophage induction in germinating spores of Streptomyces coelicolor A3(2) (φC31 ctl).. Microbiology 45:713–718
    [Google Scholar]
  15. Schachtele C. F., , De Sain C. V., Hawley L. A., Anderson D. L. 1972; Transcription during the development of bacteriophage φ29: production of host- and φ9specific ribonucleic acid.. Journal of Virology 10:1170–1178
    [Google Scholar]
  16. Suarez J. E., Chater K. F. 1980; DNA cloning in Streptomyces: a bifunctional replicon comprising pBR322 inserted into a Streptomyces phage.. Nature, London 286:527–529
    [Google Scholar]
  17. Suarez J. E., Caso J. L., Rodriguez A., Hardisson C. 1984; Structural characteristics of Streptomyces bacteriophage φC31. FEMS Microbiology Letters 22:113–117
    [Google Scholar]
  18. Thompson C. J., Ward J. M., Hopwood D. A. 1980; DNA cloning in Streptomyces: resistance genes from antibiotic-producing species.. Nature, London 286:525–527
    [Google Scholar]
  19. Westpheling J., Ranes M., Losick R. 1985; RNA polymerase heterogeneity in Streptomyces coelicolor.. Nature, London 313:22–26
    [Google Scholar]
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