%0 Journal Article %A Baudry, Bernadette %A Maurelli, Anthony T. %A Clerc, Philippe %A Sadoff, Jerald C. %A Sansonetti, Philippe J. %T Localization of Plasmid Loci Necessary for the Entry of Shigella flexneri into HeLa Cells, and Characterization of One Locus Encoding Four Immunogenic Polypeptides %D 1987 %J Microbiology, %V 133 %N 12 %P 3403-3413 %@ 1465-2080 %R https://doi.org/10.1099/00221287-133-12-3403 %I Microbiology Society, %X SUMMARY: We have previously cloned a 44 kb fragment from the virulence plasmid of Shigella flexneri serotype 5 strain M90T which is capable of restoring invasiveness to an avirulent, plasmidless mutant. This report presents a genetic and physical analysis of Tn5 mutations in recombinant clone pHS4108. Tn5 mutagenesis allowed identification of at least five regions implicated in the entry phenotype. These regions were located on a 20 kb portion of pHS4108. Expression of the insertion mutants was studied by immunoblots using the serum of a convalescent monkey infected by S. flexneri 2a, which recognized four plasmid-associated polypeptides. We propose that the four immunogenic polypeptides, a, b, c and d, are encoded by an operon. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-12-3403