1887

Abstract

A library of DNA was constructed using a cosmid cloning system. Sixteen hundred recombinant clones were generated covering the genome with a probability of 99%. Three hundred of the clones were screened for expression of antigens by a modified rocket immunoelectrophoresis technique using a polyspecific antiserum to . One clone was identified which produced the ‘common antigen’ (CA) of (Tp-4). CA shares epitopes with antigens present in more than 50 different bacterial species, but nothing is known about its structure, function and localization. The recombinant clone will be of value for a structural analysis of the CA gene.

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/content/journal/micro/10.1099/00221287-133-3-587
1987-03-01
2024-04-27
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References

  1. Axelsen N. H. 1983; Intermediate gel immunoelectrophoresis. In Handbook of Immunoprecipitation-in- gel Techniques (Scandinavian Journal of Immunology 17: suppl.10 pp 141–149
    [Google Scholar]
  2. Axelsen N. H., Bock E. 1983; Electroimmunoassay (rocket immunoelectrophoresis). In Handbook of Immunoprecipitation-in-gel Techniques (Scandinavian Journal of Immunology) 17: suppl. 10 pp 103–106
    [Google Scholar]
  3. Baseman J. B., Nichols J. C., Rumpp J. M., Hayes N. S. 1974; Purification of Treponema pallidum from infected rabbit tissue: resolution into two treponemal populations. Infection and Immunity 10:1062–1067
    [Google Scholar]
  4. Bishop N. H., Miller J. N. 1983; Humoral immune mechanism in acquired syphilis. Pathogenesis and Immunology of Treponemal Infection pp 241–269 Schell R. F., Musher D. M. Edited by New York: Dekker;
    [Google Scholar]
  5. Blattner F. R., Williams B. G., Blechl A. E., Deniston-Thompson K., Faber H. E., Furlong L.-A., Grunwald D. J., Kiefer D. O., Moore D. D., Sheldon E. L., Smithies O. 1977; Charon phages: safer derivatives of bacteriophage lambda for DNA cloning. Science 196:161–169
    [Google Scholar]
  6. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heynecker H. L., Boyer H. W., Crosa J. H., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  7. Buckel P., Zehelein E. 1981; Expression of Pseudomonas fluorescens d-galactose dehydrogenase in Escherichia coli.. Gene 16:149–159
    [Google Scholar]
  8. Burnette W. N. 1981; ‘Western blotting’: electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  9. Van Embden J. D.A., Van Der Donk R. V., Van Eijk H. G., Van Der Heide J. A., De Jong M. F., Van Olderen A. D., Osterhaus A. D., Schouls L. M. 1983; Molecular cloning and expression of Treponema pallidum DNA in Escherichia coli K-12. Infection and Immunity 42:187–196
    [Google Scholar]
  10. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166:557–580
    [Google Scholar]
  11. Hansen E. B., Pedersen E. P., Schouls L. M., Severin E., Van Embden J. D.A. 1985; Genetic characterization and partial sequence determination of a Treponema pallidum operon expressing two immunogenic membrane proteins in Escherichia coli. Journal of Bacteriology 162:1227–1237
    [Google Scholar]
  12. Hindersson P., Petersen C. S., Pedersen N. S., Høiby N., Axelsen N. H. 1984; Immunological cross-reaction between antigen Tp-4 of Treponema pallidum and an antigen common to a wide range of bacteria. Acta pathologica et microbiologica scandina- vica B92:183–188
    [Google Scholar]
  13. Hindersson P., Cockayne A., Schouls L. M., Van Embden J. D.A. 1986; Immunochemical characterization and purification of the Treponema pallidum antigen TpD expressed by Escherichia coli K12. Sexually Transmitted Diseases 13:237–244
    [Google Scholar]
  14. Hohns B., Collins J. 1980; A small cosmid for efficient cloning of large DNA fragments. Gene 11:291–298
    [Google Scholar]
  15. Høiby N. 1977; Pseudomonas aeruginosa infection in cystic fibrosis. Acta pathologica et microbiologica scandinavica, Section C suppl. no. 262 pp 1–96
    [Google Scholar]
  16. Kaijser B. 1975; Immunological studies of an antigen common to many Gram-negative bacteria with special reference to Escherichia coli. International Archives of Allergy 48:72–81
    [Google Scholar]
  17. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  18. Maniatis T., Frisch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Norgard M. V., Miller J. M. 1983; Cloning and expression of Treponema pallidum (Nichols) antigen genes in Escherichia coli. Infection and Immunity 42:186–196
    [Google Scholar]
  20. Pedersen N. S., Axelsen N. H., Petersen C. S. 1981; Antigenic analysis of Treponema pallidum: cross-reactions between individual antigens of T. pallidum and T Reiter. Scandinavian Journal of Immunology 13:143–150
    [Google Scholar]
  21. Petersen C. S., Pedersen N. S., Axelsen N. H. 1981; A simple method for the isolation of flagella from Treponema Reiter. Acta pathologica et microbiologica scandinavica C89:379–385
    [Google Scholar]
  22. Petersen C. S., Pedersen N. S., Axelsen N. H. 1982; Isolation and characterization of TR-c, an antigen of the Reiter treponeme precipitating with antibodies in syphilis. Scandinavian Journal of Immunology 15:459–465
    [Google Scholar]
  23. Sompolinsky D., Hertz J. B., Høiby N., Jensen K., Mansa B., Samra Z. 1980a; An antigen common to a wide range of bacteria. 1. The isolation of a ’common antigen’ from Pseudomonas aeruginosa. Acta pathologica et microbiologica scandinavica B88:143–149
    [Google Scholar]
  24. Sompolinsky D., Hertz J. B., Høiby N., Jensen K., Mansa B., Barkholt-Pedersen V., Samra Z. 1980b; An antigen common to a wide range of bacteria. 2. A biochemical study of a ‘common antigen’ frpm Pseudomonas aeruginosa. Acta pathoiogica et microbiologica scandinavica B88:253–260
    [Google Scholar]
  25. Stamm L., Bassford P. J. Jr 1982b; Cloning and expression of Treponema pallidum protein antigens in Escherichia coli. DNA 1:329–333
    [Google Scholar]
  26. Stamm L. V., Folds J. D., Bassford P. J. Jr 1982a; Expression of Treponema pallidum antigens in Escherichia coli K-12. Infection and Immunity 36:1238–1241
    [Google Scholar]
  27. Walfield A. M., Hanff P. A., Lovett M. A. 1982; Expression of Treponema pallidum antigens in Escherichia coli. Science 216:522–523
    [Google Scholar]
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