Isolation and Characterization of the Facultative Methylotroph Mycobacterium ID-Y Reed, William M. and Dugan, Patrick R.,, 133, 1389-1395 (1987), doi = https://doi.org/10.1099/00221287-133-5-1389, publicationName = Microbiology Society, issn = 1350-0872, abstract= SUMMARY: A facultatively methylotrophic Mycobacterium was isolated from Cleveland Harbor, Ohio, USA. The isolate, designated ID-Y, used a wide range of carbon and energy sources including methane and several other hydrocarbons. It displayed a growth cycle from rod-shaped exponential-phase cells, with many cell pairs exhibiting V-formation, to cocco-bacillary stationary-phase cells. A fixation technique involving glutaraldehyde/alcian blue resulted in the observation of a three-layered cell wall. Isolate ID-Y has an ultrastructure similar to that of other mycobacteria, particularly Mycobacterium phlei and Mycobacterium flavum, which is presently classified as a Xanthobacter species., language=, type=