1887

Abstract

The proteolytic activity of the intestinal bacterium NCDO 2217 was cell-bound during exponential growth, but was progressively released from the cells in stationary phase. Proteins hydrolysed included casein, trypsin, chymotrypsin, azocasein and the proteins in azosoya bean flour. Collagen, azocoll, elastin, gelatin, ovalbumin and bovine serum albumin were either weakly degraded or completely refractory to proteolysis. Arylamidase activity was exhibited against leucine -nitroanilide (LPNA), leucine -naphthylamide, glycyl-proline -nitroanilide and valyl-alanine -nitroanilide. The bacterium grew with ammonia, peptone or casein as sole nitrogen source. Azocasein- and LPNA-hydrolysing activities were consistently higher when grown on casein. Cell-bound protease activity increased concomitantly with growth rate in both carbon- and nitrogen-limited continuous culture. Leucine arylamidase activity was also growth-rate-dependent, being 3-fold greater at = 0·18 h compared to = 0·03 h. Extracellular proteolytic activity was only detected at low growth rates, accounting for about 25% of total protease activity.

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1988-01-01
2024-05-09
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References

  1. Appel W. 1984a; Elastase. In Methods of Enzymatic Analysis, 2nd edn.. 1 pp 1041–1045 bergmeyer H. U. Edited by London: Academic Press;
    [Google Scholar]
  2. Appel W. 1984b; Peptidases. In Methods of Enzymatic Analysis, 2nd edn.. 2 pp 949–978 bergmeyer H. U. Edited by London: Academic Press;
    [Google Scholar]
  3. Appel W. 1984c; Carboxypeptidases. In Methods of Enzymatic Analysis, 2nd edn.. 3 pp. 986–999 bergmeyer H. U. Edited by London: Academic Press;
    [Google Scholar]
  4. Bohe M., borgstrom S., Ohlsson G.K. 1983; Determination of immunoreactive trypsin, pancreatic elastase and chymotrypsin in extracts of human feces and ileostomy drainage. Digestion 27:8–15
    [Google Scholar]
  5. cotta M.A., Hespell R.B. 1986; Proteolytic activity of the ruminal bacterium Butyrivibrio fibrisolvens. . Applied and Environmental Microbiology 52:31–58
    [Google Scholar]
  6. cruickshank R. 1965 Medical Microbiology Edinburgh: Livingstone;
    [Google Scholar]
  7. Cummings J.H., gibson S.A.W., Allison C., macfarlane G.T. 1986; Proteolytic activities of colonic Bacteroides. . Proceedings of the XIV International Congress of Microbiology309
    [Google Scholar]
  8. Dowell V.R., lombard G.L. 1981; Pathogenic members of the Genus Bacteroides. . In The Prokaryotes pp 1425–1449 Starr M. P., stolp H., Truper H. G., balows A., Schlegel H. H. Edited by New York: Springer Verlag;
    [Google Scholar]
  9. finegold S.M., Sutter V.L., mathisen G.E. 1983; Normal indigenous intestinal flora. In Human Intestinal Microflora in Health and Disease pp 3–31 Hentges D. J. Edited by London: Academic Press;
    [Google Scholar]
  10. gherardini F., Babcock M., salyers A.A. 1985; Purification and characterization of two α-galactosidases associated with catabolism of guar gum and other α-galactosides by Bacteroides ovatus. . Journal of Bacteriology 161:500–506
    [Google Scholar]
  11. Gibbons R.J., macdonald J.B. 1961; Degradation of collagenous substrates by Bacteroides melaninogenicus. . Journal of Bacteriology 81:614–621
    [Google Scholar]
  12. Gul’Nik S.V., yusupova M.P., Lavrenova G.I., tartakovsky I.S., Prozorovsky S.V., stepanov V.M. 1986; Proteinases of Legionella: Phenylalanineaminopeptidase of L. pneumophila. . Journal of General Microbiology 132:387–392
    [Google Scholar]
  13. Hausmann E., kaufman E. 1969; Collagenase activity in a particulate fraction from Bacteroides melaninogenicus. . Biochimica et biophysica acta 194:612–615
    [Google Scholar]
  14. Hazlewood G.P., edwards R. 1981; Proteolytic activities of a rumen bacterium, Bacteroides rumin-icola R8/4. Journal of General Microbiology 125:11–15
    [Google Scholar]
  15. Hentges D.J., smith L.DS. 1985; Hydrolytic enzymes as virulence factors of anaerobic bacteria. In Bacterial Enzymes and Virulence pp 105–119 Holder I. A. Edited by Boca Raton, Florida: CRC Press;
    [Google Scholar]
  16. holdeman L.V., Cato E.P., moore W.E.C. eds 1977 Anaerobic Laboratory Manual, 4th edn.. Blacksburg, Virginia: VPI Anaerobe Laboratory;
    [Google Scholar]
  17. Keith S.M., herbert R.A. 1983; Dissimilatory nitrate reduction by a strain of Desulfovibrio desulfur-icans. . FEMS Microbiology Letters 18:55–59
    [Google Scholar]
  18. Loesche W.J., socransky S.S., Gibbons R.J. 1964; Bacteroides oralis, proposed new species isolated from the oral cavity of man. Journal of Bacteriology 88:1329–1337
    [Google Scholar]
  19. long S., Mothibelli M.A., robb F.T., Woods D.R. 1981; Regulation of extracellular alkaline protease activity by histidine in a collagenolytic Vibrio alginolyticus strain. Journal of General Microbiology 127:193–199
    [Google Scholar]
  20. macfarlane G.T., Allison C. 1986; Utilization of protein by human gut bacteria. FEMS Microbiology Ecology 38:19–24
    [Google Scholar]
  21. macfarlane G.T., Allison C., cummings J.H. 1985; Distribution of proteolytic activity in the intestinal contents of man. Journal of Applied Bacteriology 59:xviii–xix
    [Google Scholar]
  22. Macfarlane G.T., cummings J.H., Allison C. 1986; Protein degradation by human intestinal bacteria. Journal of General Microbiology 132:1647–1656
    [Google Scholar]
  23. macfarlane G.T., Englyst H.N. 1986; Starch utilization by the human large intestinal microflora. Journal of Applied Bacteriology 60:195–201
    [Google Scholar]
  24. macy J.M., Probst I. 1979; The biology of gastrointestinal Bacteroides. . Annual Reviews in Microbiology 33:561–594
    [Google Scholar]
  25. morihara K., Homma J.Y. 1985; Pseudomonas proteases. In Bacterial Enzymes and Virulence pp 41–79 holder I. A. Edited by Boca Raton, Florida: CRC Press;
    [Google Scholar]
  26. Pansare A.C., venugopal V., Lewis N.F. 1985; A note on nutritional influence on extracellular protease synthesis in Aeromonas hydrophila. . Journal of Applied Bacteriology 58:101–104
    [Google Scholar]
  27. salyers A.A. 1984; Bacteroides of the human lower intestinal tract. Annual Reviews in Microbiology 38:293–313
    [Google Scholar]
  28. Salyers A.A., O’Brien M. 1980; Cellular location of enzymes involved in chondroitin sulfate breakdown by Bacteroides thetaiotaomicron. . Journal of Bacteriology 143:772–780
    [Google Scholar]
  29. Salyers A.A., palmer J.K., Wilkins T.D. 1977; Laminarinase (β-glucanase) activity in Bacteroides from the human colon. Applied and Environmental Microbiology 33:1118–1124
    [Google Scholar]
  30. siefter S., Harper E. 1971; The Collagenases. In The Enzymes 3 pp 649–697 boyer P. D. Edited by New York: Academic Press;
    [Google Scholar]
  31. Van Steenbergen T.S.M., De Graaff J. 1986; Proteolytic activity of black-pigmented Bacteroides strains. FEMS Microbiology letters 33:219–222
    [Google Scholar]
  32. Whooley M.A., O’Callaghan J.A., Mcloughlin A.J. 1983; Effect of substrate on the regulation of exoprotease production by Pseudomonas aerugin-osa ATCC 10145. Journal of General Microbiology 129:981–988
    [Google Scholar]
  33. yoshimura F., Nishikata M., suzuki T., Hoover C.I., newbrun E. 1984; Characterization of a trypsin-like protease from the bacterium Bacteroides gingivalis isolated from human dental plaque. Archives of Oral Biology 29:559–564
    [Google Scholar]
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