1887

Abstract

Lipopolysaccharides (LPS) from five species of oral , strains 381 and ATCC 33277, ATCC 33269, ATCC 15930, ATCC 25611 and ATCC 33547, were extracted from whole cells by the phenol/water procedure, and subsequently purified by treatment with nuclease and ultracentrifugation. The LPS were composed of hexoses, glucosamine, fatty acids and phosphorus. Heptose and 2-keto-3-deoxyoctonate were not detected. The LPS preparations from strains 381 and ATCC 33277 presented very similar SDS-polyacrylamide gel electrophoresis patterns when stained with ammoniacal silver. They produced a fused precipitin band against an antiserum to 381 LPS in immunodiffusion tests. Antisera raised against the LPS from and reacted with the LPS prepared from all the oral strains except those of . All the LPS preparations were mitogenic for spleen cells of BALB/c (nu/nu) mice, but not for thymus cells from C3H/HeN mice. The LPS induced marked mitogenic responses and polyclonal B cell activation for spleen cells of not only C3H/HeN (LPS responder) mice, but also C3H/HeJ (LPS nonresponder) mice. The mitogenic responses were not suppressed significantly upon addition of polymyxin B to the reaction mixture. These LPS also enhanced interleukin-1 production by murine peritoneal macrophages and mouse cell line J744.1 macrophages. Hydrolysis of ATCC 33277 LPS in 1 -HCl at 100 °C for 1 h yielded lipid and polysaccharide. The lipid portion was largely composed of fatty acids and glucosamine, and was mitogenic for spleen cells from C3H/HeJ as well as C3H/HeN mice, while the polysaccharide portion induced no significant mitogenic responses under similar experimental conditions.

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1988-11-01
2024-04-23
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References

  1. Cunningham A.J., Szenberg A. 1968; Further improvements in the plaque technique for detecting single antibody-forming cells.. Immunology 14:599–600
    [Google Scholar]
  2. Daly C.G., Seymour G.J., Kieser J.B. 1980; Bacterial endotoxin: a role in chronic inflammatory periodontal disease?. Journal of Oral Pathology 9:1–15
    [Google Scholar]
  3. Duerden B.I., Goodwin L., O’Neil T.C.A. 1987; Identification of Bacteroides species from adult periodontal disease.. Journal of Medical Microbiology 24:133–137
    [Google Scholar]
  4. Galanos C., LÜderitz O., Westphal O. 1971; Preparation and properties of antisera against the lipid-A component of bacterial lipopolysaccharides.. European Journal of Biochemistry 24:116–122
    [Google Scholar]
  5. Hofstad T. 1974; The distribution of heptose and2-keto-3-deoxy-octonate in Bacteroidaceae.. Journal of General Microbiology 85:314–320
    [Google Scholar]
  6. Johns B., Bryn K. 1986; Chemical composition and biological properties of a lipopolysaccharide from Bacteroides intermedius.. Acta pathologica, microbiologica et immunologica scandinavica B94:265–271
    [Google Scholar]
  7. Joiner K.A., Mcadam K.P.W.J., Kasper D.L. 1982; Lipopolysaccharides from Bacteroides fragilis are mitogenic for spleen cells from endotoxin responder and nonresponder mice.. Infection and Immunity 36:1139–1145
    [Google Scholar]
  8. Koga T., Nishihara T., Fujiwara T., Nisizawa T., Okahashi N., Noguchi T., Hamada S. 1985; Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli.. Infection and Immunity 47:638–647
    [Google Scholar]
  9. Koga T., Odaka C., Moro I., Fujiwara T., Nishihara T., Okahashi N., Hamada S. 1987; Local Shwartzman activity of lipopolysaccharides from several selected strains of suspected periodontopathic bacteria.. Journal of Periodontal Research 22:103–107
    [Google Scholar]
  10. Kumada H., Watanabe K., Umemoto T., Haishima Y., Kondo S., Hisatsune K. 1988; Occurrence of O-phosphorylated 2-keto-3-deoxyoctonate in the lipopolysaccharide of Bacteroides gingivalis.. FEMS Microbiology Letters 51:77–80
    [Google Scholar]
  11. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature; London: 227680–685
    [Google Scholar]
  12. Mansheim B.J., Onderdonk A.B., Kasper D.L. 1978; Immunochemical and biologic studies of the lipopolysaccharide of Bacteroides melaninogenicussubspecies asaccharolyticus.. Journal of Immunology 120:72–78
    [Google Scholar]
  13. Mayrand D., Holt S.C. 1988; Biology of asaccharolytic black-pigmented Bacteroides species.. Microbiological Reviews 52:134–152
    [Google Scholar]
  14. Millar S.J., Chen P.B., Hausmann E. 1987; Monoclonal antibody for identification of Bacteroides gingivalis lipopolysaccharide.. Journal of Clinical Microbiology 25:2437–2439
    [Google Scholar]
  15. Morrison D.C. 1983; Bacterial endotoxins and pathogenesis.. Reviews of Infectious Diseases 5:S733–S747
    [Google Scholar]
  16. Nair B.C., Mayberry W.R., Dziak R., Chen P.B., Levine M.J., Hausmann E. 1983; Biological effects of a purified lipopolysaccharide from Bacteroides gingivalis.. Journal of Periodontal Research 18:40–49
    [Google Scholar]
  17. Naito Y., Okuda K., Kato T., Takazoe I. 1985; Monoclonal antibodies against surface antigens of Bacteroides gingivalis.. Infection and Immunity 50:231–235
    [Google Scholar]
  18. Okahashi N., Koga T., Nishihara T., Fujiwara T., Hamada S. 1988; Immunological properties of lipopolysaccharides isolated from Fusobacteriumnucleatum and F. necrophorum.. Journal of General Microbiology 134:1707–1715
    [Google Scholar]
  19. Slots J., Listgarten M.A. 1988; Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases.. Journal of Clinical Periodontology 15:85–93
    [Google Scholar]
  20. Tsai C.M., Frasch C.E. 1982; A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.. Analytical Biochemistry 119:115–119
    [Google Scholar]
  21. Ulevitch R.J., Johnston A.R., Weinstein D.B. 1979; New function for high density lipoproteins.. Journal of Clinical Investigation 64:1516–1524
    [Google Scholar]
  22. Weintraub A., ZÄhringer U., Lindberg A.A. 1985; Structural studies of the polysaccharide part of the cell wall lipopolysaccharide from Bacteroides fragilis NCTC 9343.. European Journal of Biochemistry 151:657–661
    [Google Scholar]
  23. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure.. Methods in Carbohydrate Chemistry 5:83–91
    [Google Scholar]
  24. Westphal O., LÜderitz O., Galanos C., Mayer H., Rietschel E.T. 1985; The story of bacterial endotoxin.. In Advances in Immunopharmacology pp. 13–34 Chedid L., Hadden J. W., Spreafico F., Dukor P., Willoughby D. Edited by Oxford: Pergamon Press;
    [Google Scholar]
  25. Wilson M., Moore J., Kieser J.B. 1986; Identity of limulus amoebocyte lysate-active root surface materials from periodontally involved teeth.. Journal of Clinical Periodontology 13:743–747
    [Google Scholar]
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