RT Journal Article SR Electronic(1) A1 André, Lars A1 Nilsson, Anders A1 Adler, LennartYR 1988 T1 The Role of Glycerol in Osmotolerance of the Yeast Debaryomyces hansenii JF Microbiology, VO 134 IS 3 SP 669 OP 677 DO https://doi.org/10.1099/00221287-134-3-669 PB Microbiology Society, SN 1465-2080, AB Transfer of growing cells of the salt-tolerant yeast Debaryomyces hansenii to media of higher salinity resulted in an increased production and intracellular accumulation of glycerol, which was proportional to the magnitude of the shift in salinity. Stress solutes other than NaCl, when added in iso-osmolar concentrations, promoted the accumulation of similar amounts of glycerol. Cells grown at high salinity rapidly lost glycerol when returned to media of lower salinity and the loss was greater when the cells were transferred to more dilute media. A mutant strain of D. hansenii showed poor glycerol production and was inhibited by NaCl at concentrations about half the maximum tolerated by the wild-type. Growth of this mutant occurred at otherwise inhibitory NaCl concentrations if the medium was supplemented with a low concentration of glycerol. The added glycerol was intracellularly accumulated to levels that increased with salinity and were only slightly lower than the corresponding wild-type levels. Glycerol additions above the growth promoting level had little effect on growth rate but caused substantial shortening of the lag phase. Osmoprotectants other than glycerol did not permit growth to occur. The mutant was isolated as a glycerol non-utilizer but displayed growth in glycerol media at increased NaCl concentrations., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-3-669