1887

Abstract

A mutant of dependent on erythromycin for growth spontaneously gives erythromycin-independent strains with altered or missing ribosomal proteins. Strains with defects in ribosome assembly were sought and obtained from among these revertants. Two organisms in which ribosomal protein L19 is altered and absent respectively have 70S ribosomes whose dissociation into sub-units is particularly sensitive to pressures generated during centrifuging. The mutant that lacks protein L19 also accumulates ribosome precursor particles during exponential growth as do others including mutants that lack proteins S20 or L1. These strains also show unbalanced synthesis of RNA and so will be useful in investigating both the pathways and the regulation of ribosome assembly.

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1988-05-01
2024-05-04
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References

  1. Bachmann B. J. 1983; Linkage map of Escherichia coli K-12,. , 7. Microbiological Reviews 47:180–230
    [Google Scholar]
  2. Barritault D., Expert-Bezancon A., Guerin M.-F., Hayes D. 1976; The use of acetone precipitation in the isolation of ribosomal proteins. European Journal of Biochemistry 63:131–135
    [Google Scholar]
  3. Butler P. D., Wild D. G. 1984; Ribosomal protein synthesis by a mutant of Escherichia coli. European Journal of Biochemistry 144:649–654
    [Google Scholar]
  4. Butler P. D., Wild D. G. 1985; The location of a mutation affecting ribosomal protein synthesis by Escherichia coli. Journal of General Microbiology 131:135–144
    [Google Scholar]
  5. Butler P. D., Sims P. F. G., Wild D. G. 1980; Intermediates in the assembly of ribosomes by a mutant of Escherichia coli. Biochemical Journal 190:157–170
    [Google Scholar]
  6. Butler P. D., Cattaneo E., Wild D. G. 1985; Interactions between mutations affecting ribosome synthesis in Escherichia coli. Journal of General Microbiology 131:945–949
    [Google Scholar]
  7. Cundliffe E. 1986; Involvement of specific portions of ribosomal RNA in defined ribosomal functions: a study utilizing antibiotics. In Structure, Function and Genetics of Ribosomes pp. 586–604 Hardesty B., Kramer G. Edited by Berlin: Springer-Verlag;
    [Google Scholar]
  8. Dabbs E. R. 1979; Selection for Escherichia coli mutants with proteins missing from the ribosome. Journal of Bacteriology 140:734–737
    [Google Scholar]
  9. Dabbs E. R. 1983; Escherichia coli kasugamycin dependence arising from mutation at the rpsI locus. Journal of Bacteriology 153:709–715
    [Google Scholar]
  10. Dabbs E. R. 1984; Organization and regulation of ribosomal protein genes in bacteria. In Genetics: New Frontiers (Proceedings of the International Congress of Genetics, 1983) pp. 35–48 New Delhi: Oxford and IBH Publishing;
    [Google Scholar]
  11. Dabbs E. R. 1986; Mutant studies on the prokaryotic ribosome. In Structure, Function and Genetics of Ribosomes pp. 733–748 Hardesty B., Kramer G. Edited by Berlin: Springer-Verlag;
    [Google Scholar]
  12. Dabbs E. R., Ehrlich R., Hasenbank R., Schroeter B.-H., Stöffler-Meilicke M., Stöffler G. 1981; Mutants of Escherichia coli lacking ribosomal protein LI. Journal of Molecular Biology 149:553–578
    [Google Scholar]
  13. Ehrenberg M., Andersson D., Bohman K., Jelenc P., Ruusala T., Kurland C. G. 1986; Ribosomal proteins tune rate and accuracy in translation. In Structure, Function and Genetics of Ribosomes pp. 573–585 Hardesty B., Kramer G. Edited by Berlin: Springer-Verlag;
    [Google Scholar]
  14. Gesteland R. F. 1966; Isolation and characterization of ribonuclease I mutants of Escherichia coli. Journal of Molecular Biology 16:67–84
    [Google Scholar]
  15. Hardy S. J. S. 1975; The stoichiometry of the ribosomal proteins of Escherichia coli. Molecular and General Genetics 14:253–274
    [Google Scholar]
  16. Herold M., Nowotny V., Dabbs E. R., Nier-Haus K. H. 1986; Assembly analysis of ribosomes from a mutant lacking the assembly-initiator protein L24: lack of L24 induces temperature sensitivity. Molecular and General Genetics 203:281–287
    [Google Scholar]
  17. Kenny J. W., Lambert J. M., Traut R. R. 1979; Cross-linking of ribosomes using 2-iminothiolane (methyl 4-mercaptobutyrimidate) and identification of cross-linked proteins by diagonal polyacrylamide/ sodium dodecyl sulfate gel electrophoresis. Methods in Enzymology 59:534–550
    [Google Scholar]
  18. Lindahl L., Zengel J. M. 1986; Ribosomal genes in Escherichia coli. Annual Review of Genetics 20:297–326
    [Google Scholar]
  19. Madjar J.-J., Michel S., Cozzone A. J., Reboud J.-P. 1979; A method to identify individual proteins in four different two-dimensional gel electrophoresis systems: application to Escherichia coli ribosomal proteins. Analytical Biochemistry 92:174–192
    [Google Scholar]
  20. Markey F., Wild D. G. 1976; An unusual precursor of 50S ribosomes in a mutant of Escherichia coli. Biochemical Journal 158:451–456
    [Google Scholar]
  21. Moazed D., Noller H. F. 1987; Interaction of antibiotics with functional sites in 16S ribosomal RNA. Nature; London: 327389–394
    [Google Scholar]
  22. Nishi K., Dabbs E. R., Schnier J. 1985; DNA sequence and complementation analysis of a mutation in the rplX gene from Escherichia coli leading to loss of ribosomal protein L24. Journal of Bacteriology 163:890–894
    [Google Scholar]
  23. Noll M., Stöffler G., Highland J. H. 1976; Dissociation of Escherichia coli ribosomes into active sub-units. Journal of Molecular Biology 108:259–264
    [Google Scholar]
  24. Nomura M., Gourse R., Baughman G. 1984; Regulation of the synthesis of ribosomes and ribosomal components. Annual Review of Biochemistry 53:75–117
    [Google Scholar]
  25. Nowotny V., Nierhaus K. H. 1982; Initiator proteins for the assembly of the 50S sub-unit from Escherichia coli ribosomes. Proceedings of the National Academy of Sciences of the United States of America 79:7238–7242
    [Google Scholar]
  26. Pande C., Wishnia A. 1986; Pressure dependence of equilibria and kinetics of Escherichia coli ribosomal sub-unit association. Journal of Biological Chemistry 261:6272–6278
    [Google Scholar]
  27. Poldermans B., Barker H., Van Knippenberg P. H. 1980; Studies on the function of two adjacent V6,,V6-dimethyladenosines near the 3’ end of 16S ribosomal RNA of Escherichia coli. IV. The effect of the methyl groups on ribosomal sub-unit interaction. Nucleic Acids Research 8:143–151
    [Google Scholar]
  28. Spörling P. F., Blackman E. 1973; Mutation to erythromycin dependence in Escherichia coli K12. Journal of Bacteriology 116:74–83
    [Google Scholar]
  29. Stöffler G., Noah M., Stöffler-Meilicke M., Dabbs E. R. 1984; The localization of protein L19 on the surface of 50S sub-units of Escherichia coli aided by the use of mutants lacking protein LI9. Journal of Biological Chemistry 259:4521–4526
    [Google Scholar]
  30. Subramanian A. R., Dabbs E. R. 1980; Functional studies on ribosomes lacking protein L1 from mutant Escherichia coli. European Journal of Biochemistry 112:425–430
    [Google Scholar]
  31. Takebe Y., Miura A., Bedwell D. M., Tam M., Nomura M. 1985; Increased expression of ribosomal genes during inhibition of ribosome assembly in Escherichia coli. Journal of Molecular Biology 184:23–30
    [Google Scholar]
  32. Wittmann H. G. 1986; Structure of ribosomes. In Structure, Function and Genetics of Ribosomes pp. 1–27 Hardesty B., Kramer G. Edited by Berlin: Springer-Verlag;
    [Google Scholar]
  33. Yusupov M. M., Spirin A. S. 1986; Are there proteins between the ribosomal sub-units? Hot tritium bombardment experiments. FEBS Letters 197:229–233
    [Google Scholar]
  34. Zamir A., Miskin R., Vogel Z., Elson D. 1974; The inactivation and reactivation of Escherichia coli ribosomes. Methods in Enzymology 30:406–426
    [Google Scholar]
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