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Volume 134, Issue 5

Cell Wall Components of as Immunomodulators: Induction of Natural Killer and Macrophage-mediated Peritoneal Cell Cytotoxicity in Mice by Mannoprotein and Glucan Fractions Free

Abstract

Cell wall components from were compared to intact cells for their ability to induce natural cytotoxic immunoeffectors in the peritoneal cavity of mice. A soluble mannoprotein extract (MP) and an insoluble glucan fraction (GG) strongly stimulated the generation of peritoneal effectors capable of lysing YAC-1 and P-815 tumour cell lines The anti-YAC-1 effectors were characterized as natural killer (NK) lymphocytes while the anti-P-815 effectors appeared to be activated macrophages. The activity of each fraction was typically dose-dependent and both fractions differed from whole cells in the kinetics of induction of cytotoxicity. However, the NK and macrophage effectors generated by these materials had similar functional and phenotypic properties, irrespective of the material used as inducer. No mannoprotein was detected in the insoluble glucan fraction GG. Hence, the immunoenhancing activity of GG could not be attributed to the presence of some MP or MP-like component. Mannan-rich fractions with low (<3%) protein content (M) or extracted by hot alkaline reagent (M-alk) were inactive as NK and macrophage inducers. Thus, the cell wall of contains at least two distinct macromolecular complexes which mediate the induction in murine peritoneal exudates of cytotoxic effectors active against tumour cell lines.

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© Society for General Microbiology, 1988
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1988-05-01
2024-05-04
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References

  1. Albersheim P., Nevius D. J., English P. D., Carr A. 1967; A method for the analysis of sugars in plant cell-wall polysaccharides by gas-liquid chromatography. Carbohydrate Research 5:340–345
     [Google Scholar]
  2. Ausiello C. M., Spagnoli G. C., Boccanera M., Casalinuovo I., Malavasi F., Casciani C. U., Cassone A. 1986; Proliferation of human peripheral blood mononuclear cells induced by Candida albicans and its cell wall fractions. Journal of Medical Microbiology 22:195–202
     [Google Scholar]
  3. Baccarini M., Bistoni F., Lohman-Matthes M. L. 1985; In vitro natural cell-mediated cytotoxicity against Candida albicans: macrophage precursors as effector cells. Journal of Immunology 134:2658–2665
     [Google Scholar]
  4. Bistoni F., Vecchiarelli A., Cenci E., Puccetti P., Marconi P., Cassone A. 1986; Evidence for macrophage-mediated protection against a lethal Candida albicans infection. Infection and Immunity 51:668–674
     [Google Scholar]
  5. Cassone A., Mattia E., Boldrini L. 1978; Agglutination of blastospores of Candida albicansby Concanavalin A and its relationship with the distribution of mannan polymers and the ultrastructure of the cell wall. Journal of General Microbiology 105:263–273
     [Google Scholar]
  6. Cassone A., Kerridge D., Gale E. F. 1979; Ultrastructural changes in the cell wall of Candida albicans following cessation of growth and their possible relationship to the development of polyene resistance. Journal of General Microbiology 110:339–349
     [Google Scholar]
  7. Cassone A., Marconi P., Bistoni F., Mattia E., Sbaraglia G., Garaci E., Bonmassar E. 1981; Immunoadjuvant effects of Candida albicans and its cell wall fractions in a mouse lymphoma model. Cancer Immunology and Immunotherapy 10:181–190
     [Google Scholar]
  8. Chen P. S., Toribara T. Y., Warner H. 1956; Microdetermination of phosphorus. Analytical Chemistry 28:1756–1758
     [Google Scholar]
  9. Cikes M., Friberg S., Klein G. 1973; Progressive loss of H-2 antigen with concomitant increase of cell surface antigen(s) determined by Moloney leukemia virus in cultured murine lymphomas. Journal of the National Cancer Institute 50:347–351
     [Google Scholar]
  10. Cutler J. E., Lloyd R. K. 1983; Enhanced antibody response induced by Candida albicans in mice. Infection and Immunity 38:1102–1108
     [Google Scholar]
  11. Domer J. E., Stashak P. W., Elkins K., Prescott B., Caldes G., Baker P. J. 1986; Separation of immunomodulatory effects of mannan from Candida albicans into stimulatory and suppressive components. Cellular Immunology 101:403–414
     [Google Scholar]
  12. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
     [Google Scholar]
  13. Gilbertson S. M., Shah P.D, Rowley D. R. 1986; NK cells suppress the generation of Lyt-2+ cytolytic T cells by suppressing or eliminating dendritic cells. Journal of Immunology 136:3567–3571
     [Google Scholar]
  14. Herberman R. B., Ortaldo J. R. 1981; Natural killer cells: their role in defense against disease. Science 24:24–30
     [Google Scholar]
  15. Jimenez B. E., Murphy J. W. 1984; In vitro effects of natural killer cells against Paracoccidioides brasiliensis yeast phase. Infection and Immunity 46:552–558
     [Google Scholar]
  16. Julius M. H., Simpson E., Herzenberg L. A. 1973; A rapid method for the isolation of functional thymus-derived murine lymphocytes. European Journal of Immunology 3:645–652
     [Google Scholar]
  17. Kasai M., Iwamori M., Nagai Y., Okomura K., Toda T. 1980; A glycolipid on the surface of mouse natural killer cells. European Journal of Immunology 10:175–181
     [Google Scholar]
  18. Kokoshis P. L., Williams D. L., Cook J. A., Diluzio N. R. 1978; Increased resistance to Staphylococcus aureus infection and enhancement of serum lysozyme activity by glucan. Science 199:1340–1342
     [Google Scholar]
  19. Landolfo S., Herberman R. B., Holden H. T. 1978; Macrophage-lymphocyte interaction in migration-inhibition factor (MIF) production against soluble or cellular tumor-associated antigens. I. Characteristics and genetic control of two different mechanisms of stimulating MIF production. Journal of Immunology 121:695–701
     [Google Scholar]
  20. Mansell P. W. A., Di Luzio N. R., Mcnamee R., Rowden G., Proctor J. W. 1976; Recognition factors and non-specific macrophage activation in the treatment of neoplastic disease. Annals of the New York Academy of Sciences 277:20–44
     [Google Scholar]
  21. Marconi P., Cassone A., Baccarini M., Tissi L., Garaci E., Bonmassar E., Frati L., Bistoni F. 1983; Relationship between tumor load, route of tumor inoculation and response to immunotherapy in a murine lymphoma model. Journal of the National Cancer Institute 71:299–335
     [Google Scholar]
  22. Marconi P., Scaringi L., Tissi L., Boccanera M., Bistoni F., Bonmassar E., Cassone A. 1985; Induction of natural killer cell activity by inactivated Candida albicans in mice. Infection and Immunity 50:297–303
     [Google Scholar]
  23. Mattia E., Cassone A. 1979; Inducibility of germ-tube formation in Candida albicans at different phases of yeast growth. Journal of General Microbiology 113:439–441
     [Google Scholar]
  24. Peat S., Whelan W. J., Edwards T. E. 1961; Polysaccharides of baker’s yeast. Part IV. Mannan. Journal of the Chemical Society29–34
     [Google Scholar]
  25. Reynolds J. A., Kastello M. D., Harrington D. H., Crobbs C. L., Peters C. J., Jenski J. V., Scott G. H., Di Luzio N. R. 1980; Glucan-induced enhancement of host resistance to selected infectious diseases. Infection and Immunity 30:51–57
     [Google Scholar]
  26. Rogers T. J., Balish E. 1980; Immunity to Candida albicans. Microbiological Reviews 44:660–684
     [Google Scholar]
  27. Santoni A., Piccoli M., Ortaldo J. R., Mason L., Wiltrout R. H., Herberman R. B. 1985; Changes in number and density of large granular lymphocytes upon in vivo augmentation of mouse natural killer activity. Journal of Immunology 134:2799–2810
     [Google Scholar]
  28. Schreiber R. D., Celada A., Buchmeier N. 1986; The role of interferon-gamma in the induction of inactivated macrophages. Annales de l’Institut Pasteur/Immunologie 137C:203–206
     [Google Scholar]
  29. Seljelid R., Bogwald J., Lundwall A. 1981; Glycan stimulation of macrophages in vitro. Experimental Cell Research 131:121–129
     [Google Scholar]
  30. Sutherland I. W., Wilkinson J. F. 1971; Chemical extraction methods of microbial cells. Methods in Microbiology 5B:345–383
     [Google Scholar]
  31. Tartof D., Check L. J., Matutis A., Hunter R. L., Fitch F. W. 1980; Studies on stimulation of cell- mediated cytotoxicity by skin test antigens. Journal of Immunology 125:2790–2796
     [Google Scholar]
  32. Tartof D., Curran J. J., Levitt D., Loken M. R. 1983; The skin test antigen stimulated killer (STAK) cell mediating NK like CMC is OKM1 positive and OKT3 negative. Clinical and Experimental Immunology 54:561–566
     [Google Scholar]
  33. Weinberg J. B., Hibbs J. B. 1979; Enhanced macrophage tumoricidal activity and tumor suppression or regression caused by heat-killed Candida albicans. Journal of the National Cancer Institute 63:1273–1278
     [Google Scholar]
  34. Williams D. L., Cook J. A., Hoffman E. O., Di Luzio N. R. 1978; Protective effect of glucan in experimentally-induced candidiasis. Journal of the Reticuloendothelial Society 23:479–490
     [Google Scholar]
  35. Zang S. R., Salup R. S., Urias P. E., Twillie T. A., Talmadge J. E., Herberman R. B., Wiltrout R. H. 1986; Augmentation of NK activity and/or macrophage-mediated cytotoxicity in the liver by biological response modifiers including human recombinant interleukin-2. Cancer Immunology and Immunotherapy 21:19–25
     [Google Scholar]
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Cell Wall Components of Candida albicans as Immunomodulators: Induction of Natural Killer and Macrophage-mediated Peritoneal Cell Cytotoxicity in Mice by Mannoprotein and Glucan Fractions
Microbiology 134, 1265 (1988); https://doi.org/10.1099/00221287-134-5-1265
/content/journal/micro/10.1099/00221287-134-5-1265
/content/journal/micro/10.1099/00221287-134-5-1265
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