%0 Journal Article %A Ratnaningsih, E. %A Dharmsthiti, S. %A Krishnapillai, V. %A Morgan, A. %A Sinclair, M. %A Holloway, B. W. %T A combined physical and genetic map of Pseudomonas aeruginosa PAO %D 1990 %J Microbiology, %V 136 %N 12 %P 2351-2357 %@ 1465-2080 %R https://doi.org/10.1099/00221287-136-12-2351 %I Microbiology Society, %X A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-12-2351