1887

Abstract

Protein antigen b (Pab) of has previously attracted interest because of its immunological and diagnostic relevance. In this study we present evidence that Pab possesses a signal sequence and is secreted from the cytoplasm of . The synthesis of Pab is enhanced under phosphate starvation indicating that the protein is involved in phosphate metabolism in .

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/content/journal/micro/10.1099/00221287-136-3-477
1990-03-01
2024-05-05
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References

  1. Andersen Å.B., Yuan Z. L., Hasløv K., Vergmann B., Bennedsen J. 1986; Interspecies reactivity of five monoclonal antibodies to Mycobacterium tuberculosis as examined by immuno-blotting and enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 23:446–451
    [Google Scholar]
  2. Andersen Å.B., Hansen E. B. 1989; Structure and mapping of antigenic domains of protein antigen b, a 38,000-molecular-weight protein of Mycobacterium tuberculosis. Infection and Immunity 57:2481–2488
    [Google Scholar]
  3. Bothamley G. H., Beck J. S., Schreuder G.M.T., D’Amaro J., De Vries R.R.P., Kardjito T., Ivanyi J. 1989; Association of tuberculosis and Mycobacterium tuberculosis-specific antibody levels with HLA. Journal of Infectious Diseases 159:549–555
    [Google Scholar]
  4. Cherayil B. J., Young R. A. 1988; A 28-kDa protein from Mycobacterium leprae is a target of the antibody response in lepromatous leprosy. Journal of Immunology 141:4370–4375
    [Google Scholar]
  5. Husson R. N., Young R. A. 1987; Genes for the major protein antigens of Mycobacterium tuberculosis:the etiologic agents of tuberculosis and leprosy share an immunodominant antigen. Proceedings of the National Academy of Sciences of the United States of America 84:1679–1683
    [Google Scholar]
  6. Kadival G. V., Chaparas S. D., Hussong D. 1987; Characterization of serologic and cell-mediated reactivity of a 38-kDa antigen isolated from Mycobacterium tuberculosis. Journal of Immunology 139:2447–2451
    [Google Scholar]
  7. Kyhse-Andersen J. 1984; Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. Journal of Biochemical and Biophysical Methods 10:203–209
    [Google Scholar]
  8. Ljungqvist L., Worsaae A., Heron I. 1988; Antibody responses against Mycobacterium tuberculosisin 11 strains of inbred mice: novel monoclonal antibody specificities generated by fusions, using spleens from BALB.B10 and CBA/J mice. Infection and Immunity 56:1994–1998
    [Google Scholar]
  9. Magota K., Otsuji N., Miki T., Horiuchi T., Tsunasawa S., Kondo J., Sakiyama F., Amemura M., Morita T., Shinagawa H., Nakata A. 1984; Nucleotide sequence of the phoS gene, the structural gene for the phosphate-binding protein of Escherichia coli. Journal of Bacteriology 157:909–917
    [Google Scholar]
  10. Matsuo K., Yamaguchi R., Yamazaki A., Tasaka H., Yamada T. 1988; Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen. Journal of Bacteriology 170:3847–3854
    [Google Scholar]
  11. Oliver D. 1985; Protein secretion in Escherichia coli. Annual Review of Microbiology 39:615–648
    [Google Scholar]
  12. Schou C., Yuan Z.-L., Andersen Å.B., Bennedsen J. 1985; Production and partial characterization of monoclonal hybridoma antibodies to Mycobacterium tuberculosis. Acta Pathologica Microbiologica et ImmunologicaScandinavica Section C Immunology 93:265–272
    [Google Scholar]
  13. Shinnick T. M., Vodkin M. H., Williams J. C. 1988; The Mycobacterium tuberculosis65-kilo-dalton antigen is a heat shock protein which corresponds to common antigen and to the Escherichia coli groEL protein. Infection and Immunity 56:446–451
    [Google Scholar]
  14. Surin B. P., Jans D. A., Fimmel A. L., Shaw D. C., Cox G. B., Rosenberg H. 1984; Structural gene for the phosphate-repressible phosphate-binding protein of Escherichia coli has its own promoter: complete nucleotide sequence of the phoS gene. Journal of Bacteriology 157:772–778
    [Google Scholar]
  15. Terasaka K., Yamaguchi R., Matsuo K., Yamazaki A., Nagai S., Yamada T. 1989; Complete nucleotide sequence of immunogenic protein MPB70 from Mycobacterium bovis BCG. FEMS Microbiology Letters 58:273–276
    [Google Scholar]
  16. Watson M.E.E. 1984; Compilation of published signal sequences. Nucleic Acids Research 12:5145–5164
    [Google Scholar]
  17. Worsaae A., Ljungqvist L., Hasløv K., Heron I., Bennedsen J. 1987; Allergenic and blastogenic reactivity of three antigens fromMycobacterium tuberculosis in sensitized guinea pigs. Infection and Immunity 55:2922–2927
    [Google Scholar]
  18. Yamaguchi R., Matsuo K., Yamazaki A., Abe C., Nagai S., Terasaka K., Yamada T. 1989; Cloning and characterization of the gene for immunogenic protein MPB64 of Mycobacterium bovis BCG. Infection and Immunity 57:283–288
    [Google Scholar]
  19. Young D., Kent L., Rees A., Lamb J., Ivanyi J. 1986; Immunological activity of a 38-kilodalton protein purified from Mycobacterium tuberculosis. Infection and Immunity 54:177–183
    [Google Scholar]
  20. Young R. A., Davis R. W. 1983; Yeast RNA polymerase II genes: isolation with antibody probes. Science 222:778–782
    [Google Scholar]
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