RT Journal Article SR Electronic(1) A1 Crasnier, Martine A1 Danchin, AntoineYR 1990 T1 Characterization of Escherichia coli adenylate cyclase mutants with modified regulation JF Microbiology, VO 136 IS 9 SP 1825 OP 1831 DO https://doi.org/10.1099/00221287-136-9-1825 PB Microbiology Society, SN 1465-2080, AB In Escherichia coli there is a large increase of cAMP synthesis in crp strains, which are deficient in the catabolite gene activator protein. In this work it was shown that this increase in cAMP synthesis does not occur in crp crr strains, deficient in both the catabolite gene activator protein and enzymelll-glucose, a component of the phosphotransferase system. It was also shown that the other components of the phosphotransferase system are required to obtain the increase of cAMP synthesis in a crp background. Adenylate cyclase mutants were obtained, by random mutagenesis, which had partial adenylate cyclase activity but which did not exhibit increased levels of cAMP in a crp background. For three mutants the mutation was identified as a single point mutation. This allowed the identification of residues arginine 188, aspartic acid 414 and glycine 463 which could be involved in the catabolite gene activator protein dependent activation process., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-9-1825