1887

Abstract

, the causative agent of Legionnaires' disease, was analysed by electron microscopy for production of surface structures. Crystalline surface (S-) layers and fimbriae were not detected, but monotrichous flagellation was seen. Polyclonal antibodies specific for the 47 kDa flagellin subunit of Philadelphia I were used in Western blots to confirm the presence of flagella subunits in various strains tested, but the antiserum also reacted with flagellin subunits of [serogroup (SG) 1 and SG2] and (SG2). Flagellation of was shown to be temperature regulated. When the growth temperature of virulent and avirulent variants of strain Philadelphia I was shifted from 30°C to either 37 or 41°C, a decrease in the percentage of flagellated bacteria within the population was observed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-8-1955
1991-08-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/8/mic-137-8-1955.html?itemId=/content/journal/micro/10.1099/00221287-137-8-1955&mimeType=html&fmt=ahah

References

  1. Aguero-Rosenfeld M. E., Yang X.-H., Nachamkin I. 1990; Infection of adult Syrian hamsters with flagellar variants of Campylobacter jejuni . Infection and Immunity 58:2214–2219
    [Google Scholar]
  2. Baskerville A., Fitzgeorge R. B., Broster ML, Hambleton P., Dennis P. J. 1981; Experimental transmission of Legionnaires’ disease by exposure to aerosols of Legionella pneumophila . Lancet ii1389–1390
    [Google Scholar]
  3. Bender L., Ott M., Marre R., Hacker J. 1990; Genome analysis of Legionella spp. by orthogonal field alternation gel electrophoresis (OFAGE). FEMS Microbiology Letters 72:253–258
    [Google Scholar]
  4. Brenner D. J. 1986; Classification of Legionellaceae: current status and remaining questions. Israel Journal of Medical Sciences 22:620–632
    [Google Scholar]
  5. Chandler F. W., Roth I. L., Callaway C. S., Bump J. L., Thomason B. M., Weaver R. E. 1980; Flagella on Legionnaires disease bacteria. Annals of Internal Medicine 93:711–714
    [Google Scholar]
  6. Catrenich C. E., Johnson W. 1988; Virulence conversion of Legionella pneumophila: a one way phenomenon. Infection and Immunity 56:3121–3125
    [Google Scholar]
  7. Cianciotto N., Eisenstein B. I., Engleberg N. C., Shuman H. 1989; Genetics and molecular pathogenesis of Legionella pneumophila, an intracellular parasite of macrophages. Molecular Biology and Medicine 6:409–424
    [Google Scholar]
  8. Cianciotto N., Eisenstein B. I., Mody C. H., Engleberg N. C. 1990; A mutation in the mip gene results in an attenuation of Legionella pneumophila virulence. Journal of Infectious Diseases 162:121–126
    [Google Scholar]
  9. Conlan J. W., Ashworth L. W. 1986; The relationship between the serogroup antigen and lipopolysaccharide of Legionella pneumophila . Journal of Hygiene 96:39–48
    [Google Scholar]
  10. Drake D., Montie T. C. 1988; Flagella, motility, and invasive virulence of Pseudomonas aeruginosa . Journal of General Microbiology 134:43–52
    [Google Scholar]
  11. Edelstein P. H. 1981; Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. Journal of Clinical Microbiology 14:298–303
    [Google Scholar]
  12. Elliott J. A., Johnson W. 1981; Immunological and biochemical relationships among flagellae isolated from Legionella pneumophila serogroups 1, 2 and 3. Infection and Immunity 33:602–610
    [Google Scholar]
  13. Elliott J. A., Johnson W. 1982; Virulence conversion of Legionella pneumophila serogroup 1 by passage in guinea pigs and embryonated eggs. Infection and Immunity 35:943–946
    [Google Scholar]
  14. Fields B. S., Sanden G. N., Barbaree J. M., Morrill W. E., Wadowsky R. M., White E. H., Feeley J. C. 1989; Intracellular multiplication of Legionella pneumophila in amoebae isolated from hospital hot water tanks. Current Microbiology 18:131–137
    [Google Scholar]
  15. Finlay B. B., Falkow S. 1989; Salmonella as an intracellular parasite. Molecular Microbiology 3:1833–1841
    [Google Scholar]
  16. Göransson M., Uhlin B. E. 1984; Environmental temperature regulates transcription of a virulence pili operon in E. coli . EMBO Journal 3:2885–2888
    [Google Scholar]
  17. Hacker J., Goebel W. 1987; Mechanisms and methods for analysing pathogenicity. Swiss Biotech 5:21–31
    [Google Scholar]
  18. Harrison T. G., Taylor A. G. 1988 A Laboratory Manual for Legionella. Chichester: Wiley;
    [Google Scholar]
  19. Hebert G. A., Callaway C. S., Ewing E. P. jr. 1984; Comparison of Legionella pneumophila, L. micdadei, L. bozemanii and L. dumoffii by transmission electron microscopy. Journal of Clinical Microbiology 19:116–121
    [Google Scholar]
  20. Hoffman P. S., Houston L., Butler C. A. 1990; Legionella pneumophila htpAB heat shock operon: nucleotide sequence and expression of the 60 kilodalton antigen in L. pneumophila infected HeLa cells. Infection and Immunity 58:3380–3387
    [Google Scholar]
  21. Isenberg H. D. 1988; Pathogenicity and virulence: another view. Clinical Microbiology Reviews 1:40–53
    [Google Scholar]
  22. Joly I. R., Chen Y. Y., Ramsay D. 1983; Serogrouping and subtyping of Legionella pneumophila with monoclonal antibodies. Journal of Clinical Microbiology 18:1040–1046
    [Google Scholar]
  23. Keen M. G., Hoffman P. S. 1989; Characterization of Legionella pneumophila extracellular protease exhibiting hemolytic and cytotoxic activities. Infection and Immunity 57:732–738
    [Google Scholar]
  24. Kilvington S., Price J. 1990; Survival of Legionella pneumophila within cysts of Acanthamoeba polyphaga following chlorine exposure. Journal of Applied Bacteriology 68:519–525
    [Google Scholar]
  25. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  26. Maurelli A. T. 1989; Temperature regulation of virulence genes in pathogenic bacteria: a general strategy for human pathogens?. Microbial Pathogenesis 7:1–10
    [Google Scholar]
  27. Messner P., Hollaus F., Sleytr U. B. 1984; Paracrystalline cell wall surface layers of different Bacillus stearothermophilus strains. International Journal of Systematic Bacteriology 34:202–210
    [Google Scholar]
  28. Peel M., Donachie W., Shaw A. 1988; Temperature-dependent expression of flagella of Listeria monocytogenes . Journal of General Microbiology 134:2171–2178
    [Google Scholar]
  29. Rodgers F. G., Greaves P. W., Macrae A. D., Lewis M. J. 1980; Electron microscopic evidence of flagella and pili on Legionella pneumophila . Journal of Clinical Pathology 33:1184–1188
    [Google Scholar]
  30. SAMBROOK J., FRITSCH E. F., MANIATIS T. 1989 Molecular Cloning: a Laboratory Manual, 2. Cold Spring Harbor NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Schmoll T., Ott M., Oudega B., Hacker J. 1990; Use of a wild- type gene fusion to determine the influence of environmental conditions on expression of the S fimbrial adhesin in an Escherichia coli pathogen. Journal of Bacteriology 172:5103–5111
    [Google Scholar]
  32. Sleytr U. B., Messner P. 1988a; Crystalline surface layers on bacteria. Crystalline Bacterial Cell Surface Layers160–186 Sleytr U. B., Messner P., Pum D., Sara M. Berlin: Springer Verlag;
    [Google Scholar]
  33. Sleytr U. B., Messner P. 1988b; Crystalline surface layers in prokaryotes. Journal of Bacteriology 170:2891–2897
    [Google Scholar]
  34. Sonesson A., Jantzen E., Bryn K., Larsson L., Eng J. 1989; Chemical composition of a lipopolysaccharide from Legionella pneumophila . Archives of Microbiology 153:72–78
    [Google Scholar]
  35. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  36. Winn W. C. jr 1988; Legionnaires disease: a historical perspective. Clinical Microbiology Reviews 1:60–81
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-8-1955
Loading
/content/journal/micro/10.1099/00221287-137-8-1955
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error